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An adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics.

Krizkova S, Fabrik I, Huska D, Adam V, Babula P, Hrabeta J, Eckschlager T, Pochop P, Darsova D, Kukacka J, Prusa R, Trnkova L, Kizek R - Int J Mol Sci (2010)

Bottom Line: The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents.This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo.The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.

ABSTRACT
The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug.

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Time-dependent changes in the concentration of MT in the plasma of two patients (a, b) with retinoblastoma. MT levels were monitored 4 or 24 hours after application of the drug and the same procedure was repeated after a few days during the next treatment with carboplatin. DP voltammograms are shown on the right.
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f7-ijms-11-04826: Time-dependent changes in the concentration of MT in the plasma of two patients (a, b) with retinoblastoma. MT levels were monitored 4 or 24 hours after application of the drug and the same procedure was repeated after a few days during the next treatment with carboplatin. DP voltammograms are shown on the right.

Mentions: Retinoblastoma is a very rare malignant disease in the Czech Republic. Its treatment is centralized into a specialized workplace: FN Motol, Prague. Samples of retinoblastoma patients (n = 2) treated long-term with carboplatin were analyzed. Figure 7 shows the changes in MT level during long-term therapy in relation to the time-dependent changes after cytostatic agent application. MT levels were monitored 4 or 24 hours after application of the drug and the same procedure was repeated after a few days during the next treatment with carboplatin. In short-time monitoring of MT concentrations, a higher increase was observed with each dose of carboplatin; nevertheless, long periods had a stabilization effect on MT levels in the plasma after carboplatin application.


An adsorptive transfer technique coupled with brdicka reaction to reveal the importance of metallothionein in chemotherapy with platinum based cytostatics.

Krizkova S, Fabrik I, Huska D, Adam V, Babula P, Hrabeta J, Eckschlager T, Pochop P, Darsova D, Kukacka J, Prusa R, Trnkova L, Kizek R - Int J Mol Sci (2010)

Time-dependent changes in the concentration of MT in the plasma of two patients (a, b) with retinoblastoma. MT levels were monitored 4 or 24 hours after application of the drug and the same procedure was repeated after a few days during the next treatment with carboplatin. DP voltammograms are shown on the right.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100849&req=5

f7-ijms-11-04826: Time-dependent changes in the concentration of MT in the plasma of two patients (a, b) with retinoblastoma. MT levels were monitored 4 or 24 hours after application of the drug and the same procedure was repeated after a few days during the next treatment with carboplatin. DP voltammograms are shown on the right.
Mentions: Retinoblastoma is a very rare malignant disease in the Czech Republic. Its treatment is centralized into a specialized workplace: FN Motol, Prague. Samples of retinoblastoma patients (n = 2) treated long-term with carboplatin were analyzed. Figure 7 shows the changes in MT level during long-term therapy in relation to the time-dependent changes after cytostatic agent application. MT levels were monitored 4 or 24 hours after application of the drug and the same procedure was repeated after a few days during the next treatment with carboplatin. In short-time monitoring of MT concentrations, a higher increase was observed with each dose of carboplatin; nevertheless, long periods had a stabilization effect on MT levels in the plasma after carboplatin application.

Bottom Line: The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents.This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo.The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.

ABSTRACT
The drugs based on platinum metals represent one of the oldest, but also one of the most effective groups of chemotherapeutic agents. Thanks to many clinical studies it is known that resistance of tumor cells to drugs is a frequent cause of chemotherapy failure. With regard to platinum based drugs, multidrug resistance can also be connected with increased expression of low-molecular weight protein metallothionein (MT). This study aimed at investigating the interactions of MT with cisplatin or carboplatin, using the adsorptive transfer technique coupled with differential pulse voltammetry Brdicka reaction (AdTS DPV Brdicka reaction), and a comparison of in vitro results with results obtained in vivo. The results obtained from the in vitro study show a strong affinity between platinum based drugs and MT. Further, we analyzed extracts of neuroblastoma cell lines treated with cisplatin or carboplatin. It is clear that neuroblastoma UKF-NB-4 cisplatin-resistant and cisplatin-sensitive cell lines unlikely respond to the presence of the platinum-based cytostatics cisplatin and carboplatin. Finally, we determined the level of MT in samples from rabbits treated with carboplatin and patients with retinoblastoma treated with the same drug.

Show MeSH
Related in: MedlinePlus