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Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation.

Tong M, Zhang L, Wang Y, Jiang H, Ren Y - Int J Mol Sci (2010)

Bottom Line: We found that Fe-chlorophyllin can promote root growth.At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control.These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).

ABSTRACT
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

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Structure of (a) hemin; and (b) Fe-chlorophyllin. R1 = H, CH3 or CH2COOH; R2 = COOH; or R1, R2 = –CH(COOH)C(O)–.
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f5-ijms-11-05246: Structure of (a) hemin; and (b) Fe-chlorophyllin. R1 = H, CH3 or CH2COOH; R2 = COOH; or R1, R2 = –CH(COOH)C(O)–.

Mentions: Nitric oxide plays various physiological roles in both plants and animals. Nitric oxide can enhance crop performance at any stage of its development including sowing, growth, flowering, and fruit formation or during many processes associated with the handling of the culture, such as transplantation, root formation in stem cuttings, or any other handling that could involve an oxidative stress condition for the plant. Nitric oxide might function downstream of the signal molecules of CO action by the maintenance of ion homeostasis and up-regulation of antioxidant defense in wheat seedling root tissues [15]. Hemin is the donor of CO in animals and plants [16,17]. We found that the structure of Fe-chlorophyllin and hemin are very similar, as shown in Figures 5a and 5b. We speculate that Fe-chlorophyllin, with the similar structure to hemin, could stimulate the production of the downstream signaling molecule nitric oxide. It is known that hemin is a useful plant growth regulator. It can enhance crop performance by application via soaking of seeds, spraying or irrigating [18]. The mechanism by which Fe-chlorophyllin promotes the growth of wheat roots will be further studied in our laboratory. Whether Fe-chlorophyllin is the donor of CO or not needs to be confirmed.


Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation.

Tong M, Zhang L, Wang Y, Jiang H, Ren Y - Int J Mol Sci (2010)

Structure of (a) hemin; and (b) Fe-chlorophyllin. R1 = H, CH3 or CH2COOH; R2 = COOH; or R1, R2 = –CH(COOH)C(O)–.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100840&req=5

f5-ijms-11-05246: Structure of (a) hemin; and (b) Fe-chlorophyllin. R1 = H, CH3 or CH2COOH; R2 = COOH; or R1, R2 = –CH(COOH)C(O)–.
Mentions: Nitric oxide plays various physiological roles in both plants and animals. Nitric oxide can enhance crop performance at any stage of its development including sowing, growth, flowering, and fruit formation or during many processes associated with the handling of the culture, such as transplantation, root formation in stem cuttings, or any other handling that could involve an oxidative stress condition for the plant. Nitric oxide might function downstream of the signal molecules of CO action by the maintenance of ion homeostasis and up-regulation of antioxidant defense in wheat seedling root tissues [15]. Hemin is the donor of CO in animals and plants [16,17]. We found that the structure of Fe-chlorophyllin and hemin are very similar, as shown in Figures 5a and 5b. We speculate that Fe-chlorophyllin, with the similar structure to hemin, could stimulate the production of the downstream signaling molecule nitric oxide. It is known that hemin is a useful plant growth regulator. It can enhance crop performance by application via soaking of seeds, spraying or irrigating [18]. The mechanism by which Fe-chlorophyllin promotes the growth of wheat roots will be further studied in our laboratory. Whether Fe-chlorophyllin is the donor of CO or not needs to be confirmed.

Bottom Line: We found that Fe-chlorophyllin can promote root growth.At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control.These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).

ABSTRACT
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

Show MeSH
Related in: MedlinePlus