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Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation.

Tong M, Zhang L, Wang Y, Jiang H, Ren Y - Int J Mol Sci (2010)

Bottom Line: We found that Fe-chlorophyllin can promote root growth.At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control.These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).

ABSTRACT
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

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Effects of Fe-chlorophyllin on the activity of IAA oxidase in the root of wheat. Seeds of wheat were soaked in the same culture solution containing 0, 0.01, 0.1, 1 and 10 mg/L Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for four days after germination. Vertical bars represent standard deviation of the mean (n = 3). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).
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f3-ijms-11-05246: Effects of Fe-chlorophyllin on the activity of IAA oxidase in the root of wheat. Seeds of wheat were soaked in the same culture solution containing 0, 0.01, 0.1, 1 and 10 mg/L Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for four days after germination. Vertical bars represent standard deviation of the mean (n = 3). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).

Mentions: The effect of Fe-chlorophyllin on IAA oxidase activity in wheat seedling roots was investigated. As shown in Figure 3, a Fe-chlorophyllin concentration-dependent change in the activity of IAA oxidase was observed. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively low Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase was remarkably decreased, being 40.1% lower than the control.


Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation.

Tong M, Zhang L, Wang Y, Jiang H, Ren Y - Int J Mol Sci (2010)

Effects of Fe-chlorophyllin on the activity of IAA oxidase in the root of wheat. Seeds of wheat were soaked in the same culture solution containing 0, 0.01, 0.1, 1 and 10 mg/L Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for four days after germination. Vertical bars represent standard deviation of the mean (n = 3). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100840&req=5

f3-ijms-11-05246: Effects of Fe-chlorophyllin on the activity of IAA oxidase in the root of wheat. Seeds of wheat were soaked in the same culture solution containing 0, 0.01, 0.1, 1 and 10 mg/L Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for four days after germination. Vertical bars represent standard deviation of the mean (n = 3). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).
Mentions: The effect of Fe-chlorophyllin on IAA oxidase activity in wheat seedling roots was investigated. As shown in Figure 3, a Fe-chlorophyllin concentration-dependent change in the activity of IAA oxidase was observed. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively low Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase was remarkably decreased, being 40.1% lower than the control.

Bottom Line: We found that Fe-chlorophyllin can promote root growth.At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control.These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).

ABSTRACT
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

Show MeSH
Related in: MedlinePlus