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Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation.

Tong M, Zhang L, Wang Y, Jiang H, Ren Y - Int J Mol Sci (2010)

Bottom Line: We found that Fe-chlorophyllin can promote root growth.At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control.These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).

ABSTRACT
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

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Fe-chlorophyllin regulates the root development of wheat (a, b). Seeds of wheat were soaked in distilled water containing various concentrations of Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for six days after germination. (a) Photograph of wheat primary root formation; (b) The length of wheat roots. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (n = 45 seedlings). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).
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f1-ijms-11-05246: Fe-chlorophyllin regulates the root development of wheat (a, b). Seeds of wheat were soaked in distilled water containing various concentrations of Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for six days after germination. (a) Photograph of wheat primary root formation; (b) The length of wheat roots. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (n = 45 seedlings). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).

Mentions: To investigate whether Fe-chlorophyllin could promote the growth of wheat roots, wheat seeds were treated with different concentrations of Fe-chlorophyllin. The results in Figure 1 show that Fe-chlorophyllin was effective in promoting the growth of wheat roots in a dose-dependent manner. For example, compared to the control alone, the addition of 0.1 mg/L Fe-chlorophyllin resulted in an increase of 2.13-fold in the length of wheat roots. However, higher concentrations of Fe-chlorophyllin contributed to inhibition of the growth of wheat roots.


Fe-chlorophyllin promotes the growth of wheat roots associated with nitric oxide generation.

Tong M, Zhang L, Wang Y, Jiang H, Ren Y - Int J Mol Sci (2010)

Fe-chlorophyllin regulates the root development of wheat (a, b). Seeds of wheat were soaked in distilled water containing various concentrations of Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for six days after germination. (a) Photograph of wheat primary root formation; (b) The length of wheat roots. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (n = 45 seedlings). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100840&req=5

f1-ijms-11-05246: Fe-chlorophyllin regulates the root development of wheat (a, b). Seeds of wheat were soaked in distilled water containing various concentrations of Fe-chlorophyllin for 12 h and then transferred to the quarter-strength Hoagland’s nutrient solution containing different concentrations of Fe-chlorophyllin for six days after germination. (a) Photograph of wheat primary root formation; (b) The length of wheat roots. Values represent the mean of three independent experiments and vertical bars indicate standard deviations (n = 45 seedlings). Asterisks indicate that mean values are significantly different between the treatment and control (p < 0.05).
Mentions: To investigate whether Fe-chlorophyllin could promote the growth of wheat roots, wheat seeds were treated with different concentrations of Fe-chlorophyllin. The results in Figure 1 show that Fe-chlorophyllin was effective in promoting the growth of wheat roots in a dose-dependent manner. For example, compared to the control alone, the addition of 0.1 mg/L Fe-chlorophyllin resulted in an increase of 2.13-fold in the length of wheat roots. However, higher concentrations of Fe-chlorophyllin contributed to inhibition of the growth of wheat roots.

Bottom Line: We found that Fe-chlorophyllin can promote root growth.At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control.These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

View Article: PubMed Central - PubMed

Affiliation: Jiangsu Key Laboratory for Supramolecular Medicinal and Applications, College of Life Science, Nanjing Normal University, Nanjing 210046, China; E-Mails: tongmin@163.com (M.T.); yifan@gmail.com (Y.W.); Jianghui523@163.com (H.J.).

ABSTRACT
Effects of Fe-chlorophyllin on the growth of wheat root were investigated in this study. We found that Fe-chlorophyllin can promote root growth. The production of nitric oxide in wheat root was detected using DAF-2DA fluorescent emission. The intensity of fluorescent in the presence of 0.1 mg/L Fe-chlorophyllin was near to that observed with the positive control of sodium nitroprusside (SNP), the nitric oxide donor. IAA oxidase activity decreased with all treatments of Fe-chlorophyllin from 0.01 to 10 mg/L. At the relatively lower Fe-chlorophyllin concentration of 0.1 mg/L, the activity of IAA oxidase displayed a remarkable decrease, being 40.1% lower than the control. Meanwhile, Fe-chlorophyllin treatment could increase the activities of reactive oxygen scavenging enzymes, such as superoxide dismutase (SOD) and peroxidase (POD), as determined using non-denaturing polyacrylamide gel electrophoresis. These results indicate that Fe-chlorophyllin contributes to the growth of wheat root associated with nitric oxide generation.

Show MeSH
Related in: MedlinePlus