Limits...
Matrix-assisted laser desorption/ionization imaging mass spectrometry.

Zaima N, Hayasaka T, Goto-Inoue N, Setou M - Int J Mol Sci (2010)

Bottom Line: The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology.MALDI-IMS has a great potential for discovery of unknown biomarkers.In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; E-Mails: thaya@hama-med.ac.jp (T.H.); naoko.goto.inoue@gmail.com (N.G.-I.); setou@hama-med.ac.jp (M.S.).

ABSTRACT
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

Show MeSH

Related in: MedlinePlus

Ion image of nutrients in rice (Oryza sativa). Scale bar: 1 mm. (a) Optical image of a rice kernel (Hinohikari). (b) Distribution of lysolecithin. (c) Distribution of triacylglycerol. (d) Merged image of lysolecithin (red) and triacylglycerol (green). The rice kernel section can be prepared using adhesive film.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3100838&req=5

f4-ijms-11-05040: Ion image of nutrients in rice (Oryza sativa). Scale bar: 1 mm. (a) Optical image of a rice kernel (Hinohikari). (b) Distribution of lysolecithin. (c) Distribution of triacylglycerol. (d) Merged image of lysolecithin (red) and triacylglycerol (green). The rice kernel section can be prepared using adhesive film.

Mentions: Food nutrients can be visualized by IMS [20,36,82,87,88]. We recently analyzed agricultural and marine products and visualized food nutrients such as γ-aminobutyric acid (GABA), essential amino acids, sucrose, α-tocopherol, γ-oryzanol, and phytic acid [36,82,87]. Volatile components can be visualized by a mass microscope equipped with an atmospheric pressure ion-source chamber (Shimadzu) [64]. Figure 4 shows the food nutrients of rice (Oryza sativa) [36]. In this study, we found it possible that nutrient distributions are different among rice varieties. The distribution of nutrients might be used as a biomarker to discriminate the geographical origin of agricultural and marine products. A larger-scale study is required to confirm our findings.


Matrix-assisted laser desorption/ionization imaging mass spectrometry.

Zaima N, Hayasaka T, Goto-Inoue N, Setou M - Int J Mol Sci (2010)

Ion image of nutrients in rice (Oryza sativa). Scale bar: 1 mm. (a) Optical image of a rice kernel (Hinohikari). (b) Distribution of lysolecithin. (c) Distribution of triacylglycerol. (d) Merged image of lysolecithin (red) and triacylglycerol (green). The rice kernel section can be prepared using adhesive film.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100838&req=5

f4-ijms-11-05040: Ion image of nutrients in rice (Oryza sativa). Scale bar: 1 mm. (a) Optical image of a rice kernel (Hinohikari). (b) Distribution of lysolecithin. (c) Distribution of triacylglycerol. (d) Merged image of lysolecithin (red) and triacylglycerol (green). The rice kernel section can be prepared using adhesive film.
Mentions: Food nutrients can be visualized by IMS [20,36,82,87,88]. We recently analyzed agricultural and marine products and visualized food nutrients such as γ-aminobutyric acid (GABA), essential amino acids, sucrose, α-tocopherol, γ-oryzanol, and phytic acid [36,82,87]. Volatile components can be visualized by a mass microscope equipped with an atmospheric pressure ion-source chamber (Shimadzu) [64]. Figure 4 shows the food nutrients of rice (Oryza sativa) [36]. In this study, we found it possible that nutrient distributions are different among rice varieties. The distribution of nutrients might be used as a biomarker to discriminate the geographical origin of agricultural and marine products. A larger-scale study is required to confirm our findings.

Bottom Line: The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology.MALDI-IMS has a great potential for discovery of unknown biomarkers.In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; E-Mails: thaya@hama-med.ac.jp (T.H.); naoko.goto.inoue@gmail.com (N.G.-I.); setou@hama-med.ac.jp (M.S.).

ABSTRACT
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

Show MeSH
Related in: MedlinePlus