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Matrix-assisted laser desorption/ionization imaging mass spectrometry.

Zaima N, Hayasaka T, Goto-Inoue N, Setou M - Int J Mol Sci (2010)

Bottom Line: The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology.MALDI-IMS has a great potential for discovery of unknown biomarkers.In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; E-Mails: thaya@hama-med.ac.jp (T.H.); naoko.goto.inoue@gmail.com (N.G.-I.); setou@hama-med.ac.jp (M.S.).

ABSTRACT
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

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Related in: MedlinePlus

Phosphatidylcholine (PC) MS/MS spectrum at m/z 798.5. Neutral loss of the PC head group and fatty acids is generally observed by MS/MS analysis of phospholipids in tissues.
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f3-ijms-11-05040: Phosphatidylcholine (PC) MS/MS spectrum at m/z 798.5. Neutral loss of the PC head group and fatty acids is generally observed by MS/MS analysis of phospholipids in tissues.

Mentions: IMS is currently the only methodology that enables simultaneous visualization of lipids. Visualization of various types of lipids, such as phospholipids [23,27,76–79], sphingolipids [28], glycolipids [13,21,80], and neutral lipids [81], has been reported. In terms of tissue sample preparation, formaldehyde fixation and OCT embedding are not recommended for lipid imaging because of a significant reduction in the quality of the mass spectra. Figure 2 shows an example of lipid imaging in a biological sample, medaka (Oryzias latipes) [23]. IMS reveals the distribution of lipids in biological tissues at the molecular species level. Phosphatidylcholine (PC) (diacyl 16:0/18:1) was observed to be localized in the brain and liver, while PC (diacyl 16:0/20:4) was intensely detected in the liver (Figures 2a and 2b). Molecular lipid species can be identified by MS/MS tissue analyses. A representative MS/MS PC spectrum is shown in Figure 3. Neutral loss of the phosphocholine head group (59 and 183 Da) and fatty acids are usually observed by MS/MS analyses of PC in tissues. Other phospholipid species commonly exhibit neutral loss of the head group (43 Da for phosphatidylethanolamine, 162 Da for phosphatidylinositol, 87 Da for phosphatidylserine, and 59 and 183 Da for sphingomyelin) on MS/MS spectra [82]. Neutral loss of fatty acids is observed in the case of MS/MS analyses for triacylglycerol [81].


Matrix-assisted laser desorption/ionization imaging mass spectrometry.

Zaima N, Hayasaka T, Goto-Inoue N, Setou M - Int J Mol Sci (2010)

Phosphatidylcholine (PC) MS/MS spectrum at m/z 798.5. Neutral loss of the PC head group and fatty acids is generally observed by MS/MS analysis of phospholipids in tissues.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100838&req=5

f3-ijms-11-05040: Phosphatidylcholine (PC) MS/MS spectrum at m/z 798.5. Neutral loss of the PC head group and fatty acids is generally observed by MS/MS analysis of phospholipids in tissues.
Mentions: IMS is currently the only methodology that enables simultaneous visualization of lipids. Visualization of various types of lipids, such as phospholipids [23,27,76–79], sphingolipids [28], glycolipids [13,21,80], and neutral lipids [81], has been reported. In terms of tissue sample preparation, formaldehyde fixation and OCT embedding are not recommended for lipid imaging because of a significant reduction in the quality of the mass spectra. Figure 2 shows an example of lipid imaging in a biological sample, medaka (Oryzias latipes) [23]. IMS reveals the distribution of lipids in biological tissues at the molecular species level. Phosphatidylcholine (PC) (diacyl 16:0/18:1) was observed to be localized in the brain and liver, while PC (diacyl 16:0/20:4) was intensely detected in the liver (Figures 2a and 2b). Molecular lipid species can be identified by MS/MS tissue analyses. A representative MS/MS PC spectrum is shown in Figure 3. Neutral loss of the phosphocholine head group (59 and 183 Da) and fatty acids are usually observed by MS/MS analyses of PC in tissues. Other phospholipid species commonly exhibit neutral loss of the head group (43 Da for phosphatidylethanolamine, 162 Da for phosphatidylinositol, 87 Da for phosphatidylserine, and 59 and 183 Da for sphingomyelin) on MS/MS spectra [82]. Neutral loss of fatty acids is observed in the case of MS/MS analyses for triacylglycerol [81].

Bottom Line: The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology.MALDI-IMS has a great potential for discovery of unknown biomarkers.In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; E-Mails: thaya@hama-med.ac.jp (T.H.); naoko.goto.inoue@gmail.com (N.G.-I.); setou@hama-med.ac.jp (M.S.).

ABSTRACT
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool that enables the simultaneous detection and identification of biomolecules in analytes. MALDI-imaging mass spectrometry (MALDI-IMS) is a two-dimensional MALDI-mass spectrometric technique used to visualize the spatial distribution of biomolecules without extraction, purification, separation, or labeling of biological samples. MALDI-IMS has revealed the characteristic distribution of several biomolecules, including proteins, peptides, amino acids, lipids, carbohydrates, and nucleotides, in various tissues. The versatility of MALDI-IMS has opened a new frontier in several fields such as medicine, agriculture, biology, pharmacology, and pathology. MALDI-IMS has a great potential for discovery of unknown biomarkers. In this review, we describe the methodology and applications of MALDI-IMS for biological samples.

Show MeSH
Related in: MedlinePlus