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Differential expression of copper-zinc superoxide dismutase gene of Polygonum sibiricum leaves, stems and underground stems, subjected to high-salt stress.

Qu CP, Xu ZR, Liu GJ, Liu C, Li Y, Wei ZG, Liu GF - Int J Mol Sci (2010)

Bottom Line: Expression analysis by real-time quantitative PCR reveals that the PS-CuZnSOD gene is expressed in leaves, stems and underground stems.PS-CuZnSOD gene expression can be induced by 3% NaHCO(3).The different mRNA levels' expression of PS-CuZnSOD show the gene's different expression modes in leaves, stems and underground stems under the salinity-alkalinity stress.

View Article: PubMed Central - PubMed

Affiliation: The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; E-Mails: qcp0451@msn.com.cn (C.-P.Q.); liuchun314@yahoo.com.cn (C.L.); liyangzaixian362@163.com (Y.L.); zhigangwe@163.com (Z.-G.W.); liuguifeng@126.com (G.-F.L.).

ABSTRACT
In aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as copper-zinc superoxide dismutase. In this work, a cDNA clone which encodes a copper-zinc superoxide dismutase gene, named PS-CuZnSOD, has been identified from P. sibiricum Laxm. by the rapid amplification of cDNA ends method (RACE). Analysis of the nucleotide sequence reveals that the PS-CuZnSOD gene cDNA clone consists of 669 bp, containing 87 bp in the 5' untranslated region; 459 bp in the open reading frame (ORF) encoding 152 amino acids; and 123 bp in 3' untranslated region. The gene accession nucleotide sequence number in GenBank is GQ472846. Sequence analysis indicates that the protein, like most plant superoxide dismutases (SOD), includes two conserved ecCuZnSOD signatures that are from the amino acids 43 to 51, and from the amino acids 137 to 148, and it has a signal peptide extension in the front of the N-terminus (1-16 aa). Expression analysis by real-time quantitative PCR reveals that the PS-CuZnSOD gene is expressed in leaves, stems and underground stems. PS-CuZnSOD gene expression can be induced by 3% NaHCO(3). The different mRNA levels' expression of PS-CuZnSOD show the gene's different expression modes in leaves, stems and underground stems under the salinity-alkalinity stress.

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Related in: MedlinePlus

Nucleotide and deduce amino acid sequences of PS-CuZnSOD cDNA from P. sibiricum Laxm. The PCR products of PS-CuZnSOD cDNA were sequenced. The full length was 669 bp, with a 5′ untranslated region of 87 bp, a 3′ untranslated region of 123 bp and an open reading frame (ORF) encoding 152 amino acid (459 bp). The cDNA sequence of PS-CuZnSOD has been submitted to GenBank (accession No. GQ472846). The rectangle indicates the active site of the PS-CuZnSOD; ellipse indicates the Cu2+ binding site; * indicates the Zn2+ binding site; # indicates the stop codon. Two conserved CuZnSOD signatures are shown in broken line. The signal peptide is shown in solid line. Two cysteines (Cys56 and Cys145) form a disulfide bond for this gene.
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f1-ijms-11-05234: Nucleotide and deduce amino acid sequences of PS-CuZnSOD cDNA from P. sibiricum Laxm. The PCR products of PS-CuZnSOD cDNA were sequenced. The full length was 669 bp, with a 5′ untranslated region of 87 bp, a 3′ untranslated region of 123 bp and an open reading frame (ORF) encoding 152 amino acid (459 bp). The cDNA sequence of PS-CuZnSOD has been submitted to GenBank (accession No. GQ472846). The rectangle indicates the active site of the PS-CuZnSOD; ellipse indicates the Cu2+ binding site; * indicates the Zn2+ binding site; # indicates the stop codon. Two conserved CuZnSOD signatures are shown in broken line. The signal peptide is shown in solid line. Two cysteines (Cys56 and Cys145) form a disulfide bond for this gene.

Mentions: In order to isolate cDNA encoding for copper-zinc superoxide dismutase, PCR reactions were performed using primers and total cDNA of plant leaves. Products of amplification were cloned and sequenced. Computer analysis, using the BLAST algorithm, confirmed that the selected sequence corresponded to a copper-zinc superoxide dismutase. The full-length copper-zinc superoxide dismutase cDNA fragment of P. sibiricum Laxm. was obtained by overlapping two cDNA fragments. The full-length copper-zinc superoxide dismutase cDNA comprised of 669 bp, containing 87 bp in the 5′-untranslated region (UTR); 459 bp in the open reading frame (ORF); and 123 bp in 3′-UTR without poly (A) tail (Figure 1). The ORF encodes a polypeptide of 152 amino acids. The calculated molecular mass of the mature protein (152 amino acids) is 15.3 kDa, with an estimated pI of 5.7. Two conserved CuZnSOD signatures are from the amino acids 43 to 51, and from the amino acids 137 to 148. The cDNA sequence and deduced amino acid sequence has been submitted to the NCBI GenBank as accession number GQ472846. Two cysteines (Cys56 and Cys145) form a disulfide bond for this gene.


Differential expression of copper-zinc superoxide dismutase gene of Polygonum sibiricum leaves, stems and underground stems, subjected to high-salt stress.

Qu CP, Xu ZR, Liu GJ, Liu C, Li Y, Wei ZG, Liu GF - Int J Mol Sci (2010)

Nucleotide and deduce amino acid sequences of PS-CuZnSOD cDNA from P. sibiricum Laxm. The PCR products of PS-CuZnSOD cDNA were sequenced. The full length was 669 bp, with a 5′ untranslated region of 87 bp, a 3′ untranslated region of 123 bp and an open reading frame (ORF) encoding 152 amino acid (459 bp). The cDNA sequence of PS-CuZnSOD has been submitted to GenBank (accession No. GQ472846). The rectangle indicates the active site of the PS-CuZnSOD; ellipse indicates the Cu2+ binding site; * indicates the Zn2+ binding site; # indicates the stop codon. Two conserved CuZnSOD signatures are shown in broken line. The signal peptide is shown in solid line. Two cysteines (Cys56 and Cys145) form a disulfide bond for this gene.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100833&req=5

f1-ijms-11-05234: Nucleotide and deduce amino acid sequences of PS-CuZnSOD cDNA from P. sibiricum Laxm. The PCR products of PS-CuZnSOD cDNA were sequenced. The full length was 669 bp, with a 5′ untranslated region of 87 bp, a 3′ untranslated region of 123 bp and an open reading frame (ORF) encoding 152 amino acid (459 bp). The cDNA sequence of PS-CuZnSOD has been submitted to GenBank (accession No. GQ472846). The rectangle indicates the active site of the PS-CuZnSOD; ellipse indicates the Cu2+ binding site; * indicates the Zn2+ binding site; # indicates the stop codon. Two conserved CuZnSOD signatures are shown in broken line. The signal peptide is shown in solid line. Two cysteines (Cys56 and Cys145) form a disulfide bond for this gene.
Mentions: In order to isolate cDNA encoding for copper-zinc superoxide dismutase, PCR reactions were performed using primers and total cDNA of plant leaves. Products of amplification were cloned and sequenced. Computer analysis, using the BLAST algorithm, confirmed that the selected sequence corresponded to a copper-zinc superoxide dismutase. The full-length copper-zinc superoxide dismutase cDNA fragment of P. sibiricum Laxm. was obtained by overlapping two cDNA fragments. The full-length copper-zinc superoxide dismutase cDNA comprised of 669 bp, containing 87 bp in the 5′-untranslated region (UTR); 459 bp in the open reading frame (ORF); and 123 bp in 3′-UTR without poly (A) tail (Figure 1). The ORF encodes a polypeptide of 152 amino acids. The calculated molecular mass of the mature protein (152 amino acids) is 15.3 kDa, with an estimated pI of 5.7. Two conserved CuZnSOD signatures are from the amino acids 43 to 51, and from the amino acids 137 to 148. The cDNA sequence and deduced amino acid sequence has been submitted to the NCBI GenBank as accession number GQ472846. Two cysteines (Cys56 and Cys145) form a disulfide bond for this gene.

Bottom Line: Expression analysis by real-time quantitative PCR reveals that the PS-CuZnSOD gene is expressed in leaves, stems and underground stems.PS-CuZnSOD gene expression can be induced by 3% NaHCO(3).The different mRNA levels' expression of PS-CuZnSOD show the gene's different expression modes in leaves, stems and underground stems under the salinity-alkalinity stress.

View Article: PubMed Central - PubMed

Affiliation: The Laboratory of Forest Genetics and Breeding and Biotechnology of Ministry of Education, Northeast Forestry University, 26 Hexing Road, Harbin 150040, China; E-Mails: qcp0451@msn.com.cn (C.-P.Q.); liuchun314@yahoo.com.cn (C.L.); liyangzaixian362@163.com (Y.L.); zhigangwe@163.com (Z.-G.W.); liuguifeng@126.com (G.-F.L.).

ABSTRACT
In aerobic organisms, protection against oxidative damage involves the combined action of highly specialized antioxidant enzymes, such as copper-zinc superoxide dismutase. In this work, a cDNA clone which encodes a copper-zinc superoxide dismutase gene, named PS-CuZnSOD, has been identified from P. sibiricum Laxm. by the rapid amplification of cDNA ends method (RACE). Analysis of the nucleotide sequence reveals that the PS-CuZnSOD gene cDNA clone consists of 669 bp, containing 87 bp in the 5' untranslated region; 459 bp in the open reading frame (ORF) encoding 152 amino acids; and 123 bp in 3' untranslated region. The gene accession nucleotide sequence number in GenBank is GQ472846. Sequence analysis indicates that the protein, like most plant superoxide dismutases (SOD), includes two conserved ecCuZnSOD signatures that are from the amino acids 43 to 51, and from the amino acids 137 to 148, and it has a signal peptide extension in the front of the N-terminus (1-16 aa). Expression analysis by real-time quantitative PCR reveals that the PS-CuZnSOD gene is expressed in leaves, stems and underground stems. PS-CuZnSOD gene expression can be induced by 3% NaHCO(3). The different mRNA levels' expression of PS-CuZnSOD show the gene's different expression modes in leaves, stems and underground stems under the salinity-alkalinity stress.

Show MeSH
Related in: MedlinePlus