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3,4-Methylenedioxymethamphetamine alters left ventricular function and activates nuclear factor-Kappa B (NF-κB) in a time and dose dependent manner.

Tiangco DA, Halcomb S, Lattanzio FA, Hargrave BY - Int J Mol Sci (2010)

Bottom Line: 3,4-Methylenedioxymethamphetamine (MDMA) is an illicit psychoactive drug with cardiovascular effects that have not been fully described.We also observed the effects of MDMA on nuclear factor-kappa B (NF-κB) activity in cultured rat ventricular myocytes (H9c2).The current results suggest that MDMA is acutely detrimental to heart function and that an intact cardiovascular NOS system is important to help mitigate early sequelae in some functional parameters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, USA.

ABSTRACT
3,4-Methylenedioxymethamphetamine (MDMA) is an illicit psychoactive drug with cardiovascular effects that have not been fully described. In the current study, we observed the effects of acute MDMA on rabbit left ventricular function. We also observed the effects of MDMA on nuclear factor-kappa B (NF-κB) activity in cultured rat ventricular myocytes (H9c2). In the rabbit, MDMA (2 mg/kg) alone caused a significant increase in heart rate and a significant decrease in the duration of the cardiac cycle. Inhibition of nitric oxide synthase (NOS) by pretreatment with L-NAME (10 mg/kg) alone caused significant dysfunction in heart rate, systolic pressure, diastolic pressure, duration of relaxation, duration of cardiac cycle, and mean left ventricular pressure. Pretreatment with L-NAME followed by treatment with MDMA caused significant dysfunction in additional parameters that were not abnormal upon exposure to either compound in isolation: duration of contraction, inotropy, and pulse pressure. Exposure to 1.0 mM MDMA for 6 h or 2.0 μM MDMA for 12 h caused increased nuclear localization of NF-κB in cultured H9c2 cells. The current results suggest that MDMA is acutely detrimental to heart function and that an intact cardiovascular NOS system is important to help mitigate early sequelae in some functional parameters. The delayed timing of NF-κB activation suggests that this factor may be relevant to MDMA induced cardiomyopathy of later onset.

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Normalized dP/dt (mean ± SD). Placebo group (n = 5) received sterile isotonic saline. MDMA group (n = 7) received 2 mg/kg MDMA. L-NAME + MDMA group (n = 4) received 10 mg/kg L-NAME for 10 minutes followed by 2 mg/kg MDMA. ^ p < 0.05 compared to MDMA.
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f9-ijms-11-04843: Normalized dP/dt (mean ± SD). Placebo group (n = 5) received sterile isotonic saline. MDMA group (n = 7) received 2 mg/kg MDMA. L-NAME + MDMA group (n = 4) received 10 mg/kg L-NAME for 10 minutes followed by 2 mg/kg MDMA. ^ p < 0.05 compared to MDMA.

Mentions: In the group of animals pretreated with L-NAME and then given MDMA (L-NAME + MDMA), heart rate (Figure 1) was significantly decreased while systolic and diastolic pressures, duration of contraction, duration of relaxation, duration of cardiac cycle, mean pressure, and pulse pressure were all significantly elevated from baseline (Figures 2–9). dP/dt showed a significant increase relative to the MDMA group at the 1 min mark.


3,4-Methylenedioxymethamphetamine alters left ventricular function and activates nuclear factor-Kappa B (NF-κB) in a time and dose dependent manner.

Tiangco DA, Halcomb S, Lattanzio FA, Hargrave BY - Int J Mol Sci (2010)

Normalized dP/dt (mean ± SD). Placebo group (n = 5) received sterile isotonic saline. MDMA group (n = 7) received 2 mg/kg MDMA. L-NAME + MDMA group (n = 4) received 10 mg/kg L-NAME for 10 minutes followed by 2 mg/kg MDMA. ^ p < 0.05 compared to MDMA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100831&req=5

f9-ijms-11-04843: Normalized dP/dt (mean ± SD). Placebo group (n = 5) received sterile isotonic saline. MDMA group (n = 7) received 2 mg/kg MDMA. L-NAME + MDMA group (n = 4) received 10 mg/kg L-NAME for 10 minutes followed by 2 mg/kg MDMA. ^ p < 0.05 compared to MDMA.
Mentions: In the group of animals pretreated with L-NAME and then given MDMA (L-NAME + MDMA), heart rate (Figure 1) was significantly decreased while systolic and diastolic pressures, duration of contraction, duration of relaxation, duration of cardiac cycle, mean pressure, and pulse pressure were all significantly elevated from baseline (Figures 2–9). dP/dt showed a significant increase relative to the MDMA group at the 1 min mark.

Bottom Line: 3,4-Methylenedioxymethamphetamine (MDMA) is an illicit psychoactive drug with cardiovascular effects that have not been fully described.We also observed the effects of MDMA on nuclear factor-kappa B (NF-κB) activity in cultured rat ventricular myocytes (H9c2).The current results suggest that MDMA is acutely detrimental to heart function and that an intact cardiovascular NOS system is important to help mitigate early sequelae in some functional parameters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biological Sciences, Old Dominion University, Norfolk, VA 23529, USA.

ABSTRACT
3,4-Methylenedioxymethamphetamine (MDMA) is an illicit psychoactive drug with cardiovascular effects that have not been fully described. In the current study, we observed the effects of acute MDMA on rabbit left ventricular function. We also observed the effects of MDMA on nuclear factor-kappa B (NF-κB) activity in cultured rat ventricular myocytes (H9c2). In the rabbit, MDMA (2 mg/kg) alone caused a significant increase in heart rate and a significant decrease in the duration of the cardiac cycle. Inhibition of nitric oxide synthase (NOS) by pretreatment with L-NAME (10 mg/kg) alone caused significant dysfunction in heart rate, systolic pressure, diastolic pressure, duration of relaxation, duration of cardiac cycle, and mean left ventricular pressure. Pretreatment with L-NAME followed by treatment with MDMA caused significant dysfunction in additional parameters that were not abnormal upon exposure to either compound in isolation: duration of contraction, inotropy, and pulse pressure. Exposure to 1.0 mM MDMA for 6 h or 2.0 μM MDMA for 12 h caused increased nuclear localization of NF-κB in cultured H9c2 cells. The current results suggest that MDMA is acutely detrimental to heart function and that an intact cardiovascular NOS system is important to help mitigate early sequelae in some functional parameters. The delayed timing of NF-κB activation suggests that this factor may be relevant to MDMA induced cardiomyopathy of later onset.

Show MeSH
Related in: MedlinePlus