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Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections.

Cotar AI, Chifiriuc MC, Dinu S, Bucur M, Iordache C, Banu O, Dracea O, Larion C, Lazar V - Int J Mol Sci (2010)

Bottom Line: Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts.The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens.Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research in Microbiology and Immunology, Cantacuzino, Spl. Independentei 103, cod 060631, Bucharest 060101, Romania; E-Mails: aniioana@yahoo.com (A.-I.C.); carmeniordache78@yahoo.com (C.I.); olgutza_dracea@yahoo.co.uk (O.D.); larioncristina@yahoo.com (C.L.).

ABSTRACT
Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

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Related in: MedlinePlus

Gel electrophoresis of amplified products of fnbA gene. Line 1: Bench Top 100 bp DNA Lader (Promega); 2: S. aureus 10936; 3: S. aureus 11372; 4: S. aureus 11327; 5: MRSA 11325; 6: MRSA 11573; 7: MRSA 11047; 8: S. aureus 5/06; 9: S. aureus 9/06; 10: S. aureus 11323; and 11: negative control (pure water).
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f3-ijms-11-05273: Gel electrophoresis of amplified products of fnbA gene. Line 1: Bench Top 100 bp DNA Lader (Promega); 2: S. aureus 10936; 3: S. aureus 11372; 4: S. aureus 11327; 5: MRSA 11325; 6: MRSA 11573; 7: MRSA 11047; 8: S. aureus 5/06; 9: S. aureus 9/06; 10: S. aureus 11323; and 11: negative control (pure water).

Mentions: The results of PCR analysis concerning the presence of the fnbA gene showed that all S. aureus strains possess this gene (Figure 3). Fnbps were shown to be required for the internalization S. aureus into nonprofessional phagocytic cells. Different studies showed that efficient invasion of S. aureus is critically dependent on one of the two known forms of Fnbps, FnbpA or FnbpB. Finally, the results of PCR analysis concerning the presence of cna gene, demonstrated the polymophism of this gene among the studied S. aureus strains. Seven strains possess a 1120 bp gene, whereas two exhibited a variant of 560 bp gene (Figure 4). The results of PCR assays for adhesins genes showed that all studied S. aureus strains possess: elastin-binding protein, clumping factors A and B, fibronectin-binding protein A, fibrinogen-binding protein and collagen-binding protein. The presence of these adhesins demonstrates the involvement of the respective virulence factors in the determined S. aureus infections associated with cardiovascular devices.


Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections.

Cotar AI, Chifiriuc MC, Dinu S, Bucur M, Iordache C, Banu O, Dracea O, Larion C, Lazar V - Int J Mol Sci (2010)

Gel electrophoresis of amplified products of fnbA gene. Line 1: Bench Top 100 bp DNA Lader (Promega); 2: S. aureus 10936; 3: S. aureus 11372; 4: S. aureus 11327; 5: MRSA 11325; 6: MRSA 11573; 7: MRSA 11047; 8: S. aureus 5/06; 9: S. aureus 9/06; 10: S. aureus 11323; and 11: negative control (pure water).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100824&req=5

f3-ijms-11-05273: Gel electrophoresis of amplified products of fnbA gene. Line 1: Bench Top 100 bp DNA Lader (Promega); 2: S. aureus 10936; 3: S. aureus 11372; 4: S. aureus 11327; 5: MRSA 11325; 6: MRSA 11573; 7: MRSA 11047; 8: S. aureus 5/06; 9: S. aureus 9/06; 10: S. aureus 11323; and 11: negative control (pure water).
Mentions: The results of PCR analysis concerning the presence of the fnbA gene showed that all S. aureus strains possess this gene (Figure 3). Fnbps were shown to be required for the internalization S. aureus into nonprofessional phagocytic cells. Different studies showed that efficient invasion of S. aureus is critically dependent on one of the two known forms of Fnbps, FnbpA or FnbpB. Finally, the results of PCR analysis concerning the presence of cna gene, demonstrated the polymophism of this gene among the studied S. aureus strains. Seven strains possess a 1120 bp gene, whereas two exhibited a variant of 560 bp gene (Figure 4). The results of PCR assays for adhesins genes showed that all studied S. aureus strains possess: elastin-binding protein, clumping factors A and B, fibronectin-binding protein A, fibrinogen-binding protein and collagen-binding protein. The presence of these adhesins demonstrates the involvement of the respective virulence factors in the determined S. aureus infections associated with cardiovascular devices.

Bottom Line: Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts.The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens.Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research in Microbiology and Immunology, Cantacuzino, Spl. Independentei 103, cod 060631, Bucharest 060101, Romania; E-Mails: aniioana@yahoo.com (A.-I.C.); carmeniordache78@yahoo.com (C.I.); olgutza_dracea@yahoo.co.uk (O.D.); larioncristina@yahoo.com (C.L.).

ABSTRACT
Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

Show MeSH
Related in: MedlinePlus