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Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections.

Cotar AI, Chifiriuc MC, Dinu S, Bucur M, Iordache C, Banu O, Dracea O, Larion C, Lazar V - Int J Mol Sci (2010)

Bottom Line: Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts.The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens.Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research in Microbiology and Immunology, Cantacuzino, Spl. Independentei 103, cod 060631, Bucharest 060101, Romania; E-Mails: aniioana@yahoo.com (A.-I.C.); carmeniordache78@yahoo.com (C.I.); olgutza_dracea@yahoo.co.uk (O.D.); larioncristina@yahoo.com (C.L.).

ABSTRACT
Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

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Related in: MedlinePlus

Ethidium bromide-stained 1.5% agarose gel showing the amplified products of algD gene. Lines 1 and 12: DNA ladder 100bp (Promega); 2: Ps. aeruginosa 101; 3: Ps. aeruginosa 1558; 4: Ps. aeruginosa 111; 5: Ps. aeruginosa 1443; 6: Ps. aeruginosa 1093; 7: negative control (pure water); 8: Ps. aeruginosa 1561; 9: Ps. aeruginosa 20; 10: Ps. aeruginosa 1442; 11: Ps. aeruginosa 84; and 13: Ps. aeruginosa 1562.
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f14-ijms-11-05273: Ethidium bromide-stained 1.5% agarose gel showing the amplified products of algD gene. Lines 1 and 12: DNA ladder 100bp (Promega); 2: Ps. aeruginosa 101; 3: Ps. aeruginosa 1558; 4: Ps. aeruginosa 111; 5: Ps. aeruginosa 1443; 6: Ps. aeruginosa 1093; 7: negative control (pure water); 8: Ps. aeruginosa 1561; 9: Ps. aeruginosa 20; 10: Ps. aeruginosa 1442; 11: Ps. aeruginosa 84; and 13: Ps. aeruginosa 1562.

Mentions: We have detected by PCR analysis 15 virulence genes in S. aureus, respectively 7 in Ps. aeruginosa strains isolated from infections with different clinical pictures. The results of PCR assays are presented in Figures 1–14.


Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections.

Cotar AI, Chifiriuc MC, Dinu S, Bucur M, Iordache C, Banu O, Dracea O, Larion C, Lazar V - Int J Mol Sci (2010)

Ethidium bromide-stained 1.5% agarose gel showing the amplified products of algD gene. Lines 1 and 12: DNA ladder 100bp (Promega); 2: Ps. aeruginosa 101; 3: Ps. aeruginosa 1558; 4: Ps. aeruginosa 111; 5: Ps. aeruginosa 1443; 6: Ps. aeruginosa 1093; 7: negative control (pure water); 8: Ps. aeruginosa 1561; 9: Ps. aeruginosa 20; 10: Ps. aeruginosa 1442; 11: Ps. aeruginosa 84; and 13: Ps. aeruginosa 1562.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100824&req=5

f14-ijms-11-05273: Ethidium bromide-stained 1.5% agarose gel showing the amplified products of algD gene. Lines 1 and 12: DNA ladder 100bp (Promega); 2: Ps. aeruginosa 101; 3: Ps. aeruginosa 1558; 4: Ps. aeruginosa 111; 5: Ps. aeruginosa 1443; 6: Ps. aeruginosa 1093; 7: negative control (pure water); 8: Ps. aeruginosa 1561; 9: Ps. aeruginosa 20; 10: Ps. aeruginosa 1442; 11: Ps. aeruginosa 84; and 13: Ps. aeruginosa 1562.
Mentions: We have detected by PCR analysis 15 virulence genes in S. aureus, respectively 7 in Ps. aeruginosa strains isolated from infections with different clinical pictures. The results of PCR assays are presented in Figures 1–14.

Bottom Line: Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts.The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens.Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

View Article: PubMed Central - PubMed

Affiliation: National Institute for Research in Microbiology and Immunology, Cantacuzino, Spl. Independentei 103, cod 060631, Bucharest 060101, Romania; E-Mails: aniioana@yahoo.com (A.-I.C.); carmeniordache78@yahoo.com (C.I.); olgutza_dracea@yahoo.co.uk (O.D.); larioncristina@yahoo.com (C.L.).

ABSTRACT
Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

Show MeSH
Related in: MedlinePlus