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Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

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Fraction 3 inhibits UVB irradiation increased PCNA expression. (A) PCNA expression by immunohistochemistry, magnification 400 X. (B) Quantification of PCNA expression by counting positive staining cells, mean ± S.E. (n = 4). *p < 0.05 compared to the control group.
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f7-ijms-11-04782: Fraction 3 inhibits UVB irradiation increased PCNA expression. (A) PCNA expression by immunohistochemistry, magnification 400 X. (B) Quantification of PCNA expression by counting positive staining cells, mean ± S.E. (n = 4). *p < 0.05 compared to the control group.

Mentions: PCNA participates in the synthesis and metabolism of nucleic acid and plays an important role in DNA recombination, reproduction and repair. As DNA damage occurs, the expression of PCNA increases and executes the function to repair DNA. Therefore, PCNA expression is a marker of DNA repair and could be applied as an indicator of UVB-induced damage. As shown in Figure 7, after UVB exposure, the expression of PCNA increased ,which could be inhibited by pre-treatment with Fraction 3 (1 and 3 mg/mL) The PCNA expression decreased about 35 and 50% after treatment with 1 and 3 mg/mL of Fraction 3, respectively (Figure 7A and B). Thus, Fracton 3 could prevent the DNA damage by UVB exposure, which might contribute to decrease the PCNA expression [27].


Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Fraction 3 inhibits UVB irradiation increased PCNA expression. (A) PCNA expression by immunohistochemistry, magnification 400 X. (B) Quantification of PCNA expression by counting positive staining cells, mean ± S.E. (n = 4). *p < 0.05 compared to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100816&req=5

f7-ijms-11-04782: Fraction 3 inhibits UVB irradiation increased PCNA expression. (A) PCNA expression by immunohistochemistry, magnification 400 X. (B) Quantification of PCNA expression by counting positive staining cells, mean ± S.E. (n = 4). *p < 0.05 compared to the control group.
Mentions: PCNA participates in the synthesis and metabolism of nucleic acid and plays an important role in DNA recombination, reproduction and repair. As DNA damage occurs, the expression of PCNA increases and executes the function to repair DNA. Therefore, PCNA expression is a marker of DNA repair and could be applied as an indicator of UVB-induced damage. As shown in Figure 7, after UVB exposure, the expression of PCNA increased ,which could be inhibited by pre-treatment with Fraction 3 (1 and 3 mg/mL) The PCNA expression decreased about 35 and 50% after treatment with 1 and 3 mg/mL of Fraction 3, respectively (Figure 7A and B). Thus, Fracton 3 could prevent the DNA damage by UVB exposure, which might contribute to decrease the PCNA expression [27].

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

Show MeSH
Related in: MedlinePlus