Limits...
Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

Show MeSH

Related in: MedlinePlus

Effects of Fraction 3 on catalase activity. Data is shown as mean ± S.E. (n = 4). *p < 0.05 compared to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3100816&req=5

f5-ijms-11-04782: Effects of Fraction 3 on catalase activity. Data is shown as mean ± S.E. (n = 4). *p < 0.05 compared to the control group.

Mentions: UVB exposure could elicit H2O2 production. Nevertheless, H2O2 was converted to oxygen and water by catalase so UVB exposure might contribute to the decrease of the catalase activity [12]. Moreover, catalase is able to reduce oxidative stress. In our results, after UVB exposure, catalase was significantly depleted in the dorsal skin of ICR-Foxn/nu mice. Compared with the non-exposure group, the catalase activity was reduced by about 40% in the UVB exposure group. However, treatment with Fraction 3 (1 and 3 mg/mL) significantly enhanced the catalase activity and there was no notable difference between 1 mg/mL and the non-exposure group (Figure 5). In addition, compared with our recent study, when treated with 1 mg/mL of Fraction 3, the average activity of catalase (92%) was higher than with treatment with 1 mg/mL of ISO-1 (85%). In addition, treatment with genistein could not increase the catalase activity [12]. Hence, the results suggested that Fraction 3 possesses the highest efficiency in reducing oxidative stress. Chiang et al. [5] pointed out that soy isoflavone extract could inhibit UVB-induced H2O2 production in keratinocytes. Consequently, we suggest that the effect of increasing catalase activity of Fraction 3 might be attributed to diminution of intracellular H2O2 production.


Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Effects of Fraction 3 on catalase activity. Data is shown as mean ± S.E. (n = 4). *p < 0.05 compared to the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100816&req=5

f5-ijms-11-04782: Effects of Fraction 3 on catalase activity. Data is shown as mean ± S.E. (n = 4). *p < 0.05 compared to the control group.
Mentions: UVB exposure could elicit H2O2 production. Nevertheless, H2O2 was converted to oxygen and water by catalase so UVB exposure might contribute to the decrease of the catalase activity [12]. Moreover, catalase is able to reduce oxidative stress. In our results, after UVB exposure, catalase was significantly depleted in the dorsal skin of ICR-Foxn/nu mice. Compared with the non-exposure group, the catalase activity was reduced by about 40% in the UVB exposure group. However, treatment with Fraction 3 (1 and 3 mg/mL) significantly enhanced the catalase activity and there was no notable difference between 1 mg/mL and the non-exposure group (Figure 5). In addition, compared with our recent study, when treated with 1 mg/mL of Fraction 3, the average activity of catalase (92%) was higher than with treatment with 1 mg/mL of ISO-1 (85%). In addition, treatment with genistein could not increase the catalase activity [12]. Hence, the results suggested that Fraction 3 possesses the highest efficiency in reducing oxidative stress. Chiang et al. [5] pointed out that soy isoflavone extract could inhibit UVB-induced H2O2 production in keratinocytes. Consequently, we suggest that the effect of increasing catalase activity of Fraction 3 might be attributed to diminution of intracellular H2O2 production.

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

Show MeSH
Related in: MedlinePlus