Limits...
Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

Show MeSH

Related in: MedlinePlus

Fraction 3 inhibits UVB-induced epidermal proliferation, *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC3100816&req=5

f4-ijms-11-04782: Fraction 3 inhibits UVB-induced epidermal proliferation, *p < 0.05.

Mentions: The epidermis growth factor receptor (EGFR) can be activated by UVB exposure leading to an increase of the thickness of the epidermis [20]. In our results, the epidermal thickness of the dorsal skin of mice of the control group was 13.08 ± 0.65 μm and of the UVB exposure group was 36.20 ± 1.79 μm. However, the epidermal thickness was significantly diminished by treatment with Fraction 3 and the average epidermal thickness was 20.94 ± 4.32 (1 mg/mL) and 17.14 ± 0.70 (3 mg/mL). Compared with the UVB exposure group, treatment with 1 and 3 mg/mL of Fraction 3 could reduce the epidermal thickness by about 43 and 53%, respectively (Figure 4). The EGFR has been reported to promote epidermal hyperplasia and the epidermal thickness in normal mice was higher than that of mice lacking EGFR after UVB exposure [20]. According to our results, the reduced epidermal thickness by Fraction 3 might be related to its interference with EGFR expression.


Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Fraction 3 inhibits UVB-induced epidermal proliferation, *p < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100816&req=5

f4-ijms-11-04782: Fraction 3 inhibits UVB-induced epidermal proliferation, *p < 0.05.
Mentions: The epidermis growth factor receptor (EGFR) can be activated by UVB exposure leading to an increase of the thickness of the epidermis [20]. In our results, the epidermal thickness of the dorsal skin of mice of the control group was 13.08 ± 0.65 μm and of the UVB exposure group was 36.20 ± 1.79 μm. However, the epidermal thickness was significantly diminished by treatment with Fraction 3 and the average epidermal thickness was 20.94 ± 4.32 (1 mg/mL) and 17.14 ± 0.70 (3 mg/mL). Compared with the UVB exposure group, treatment with 1 and 3 mg/mL of Fraction 3 could reduce the epidermal thickness by about 43 and 53%, respectively (Figure 4). The EGFR has been reported to promote epidermal hyperplasia and the epidermal thickness in normal mice was higher than that of mice lacking EGFR after UVB exposure [20]. According to our results, the reduced epidermal thickness by Fraction 3 might be related to its interference with EGFR expression.

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

Show MeSH
Related in: MedlinePlus