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Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

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(A) Fraction 3 was not cytotoxic to keratinocytes, (□) ISO-1; (▪) Fraction 3; (▪) genistein. UVB irradiation-induced cell death decreased after treatment with ISO-1 and Fraction 3 by MTT assay. Results in (A) are expressed as percentage of control and the mean ± S.E. (n = 3). *P < 0.05 vs. control. (B) Effects of Fraction 3 and ISO-1 on UVB-induced ERK1/2, p38 and JNK phosphorylation. HaCaT cells were preincubated with Fraction 3 and ISO-1 for 24 h, followed by UVB irradiation. After incubation, cells were collected and lysates were analyzed by Western blot analysis, (F) Fraction 3; (I) ISO-1.
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f1-ijms-11-04782: (A) Fraction 3 was not cytotoxic to keratinocytes, (□) ISO-1; (▪) Fraction 3; (▪) genistein. UVB irradiation-induced cell death decreased after treatment with ISO-1 and Fraction 3 by MTT assay. Results in (A) are expressed as percentage of control and the mean ± S.E. (n = 3). *P < 0.05 vs. control. (B) Effects of Fraction 3 and ISO-1 on UVB-induced ERK1/2, p38 and JNK phosphorylation. HaCaT cells were preincubated with Fraction 3 and ISO-1 for 24 h, followed by UVB irradiation. After incubation, cells were collected and lysates were analyzed by Western blot analysis, (F) Fraction 3; (I) ISO-1.

Mentions: Cell viability was 95–100% when treated with the soy isoflavone extract Fraction 3 (Fraction 3), which contains both the aglycone and acetylglucoside forms of isoflavones. The result indicated that soy isoflavone extract Fraction 3 from soy cake is non-toxic for human keratinocytes. In order to explore the photoprotective effects of Fraction 3 on keratinocytes, cells were treated with Fraction 3 (3, 5, 7.5, 10 μg/mL). In control cells, the cell viability significantly decreased after UVB exposure. However, treatment of cells with Fraction 3 obviously increased the cell viability. With the optimal concentration, 5 μg/mL, the cell viability increased about 12% (compare with the control UVB exposure group). The protective effects had no significant difference between 3 and 7.5 μg/mL, and thus the results suggest that Fraction 3 still had a protective effect at a lower concentration (3 μg/mL). In contrast, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) had no significant protective effects on UVB-induced keratinocyte death at the concentration of 3 μg/mL. Moreover, genistein decreased the cell viability in a dose-dependent manner (Figure 1A). In Western blot analysis, the levels of phosphorylation of ERK1/2, p38 and JNK in cells treated with Fraction 3 (3 and 5 μg/mL) were lower than those treated with ISO-1 (3 and 5 μg/mL; Figure 1B). Therefore, Fraction 3 possessed more protective effects on UVB-induced keratinocyte death than ISO-1 and genistein.


Anti-photoaging effects of soy isoflavone extract (aglycone and acetylglucoside form) from soybean cake.

Huang CC, Hsu BY, Wu NL, Tsui WH, Lin TJ, Su CC, Hung CF - Int J Mol Sci (2010)

(A) Fraction 3 was not cytotoxic to keratinocytes, (□) ISO-1; (▪) Fraction 3; (▪) genistein. UVB irradiation-induced cell death decreased after treatment with ISO-1 and Fraction 3 by MTT assay. Results in (A) are expressed as percentage of control and the mean ± S.E. (n = 3). *P < 0.05 vs. control. (B) Effects of Fraction 3 and ISO-1 on UVB-induced ERK1/2, p38 and JNK phosphorylation. HaCaT cells were preincubated with Fraction 3 and ISO-1 for 24 h, followed by UVB irradiation. After incubation, cells were collected and lysates were analyzed by Western blot analysis, (F) Fraction 3; (I) ISO-1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC3100816&req=5

f1-ijms-11-04782: (A) Fraction 3 was not cytotoxic to keratinocytes, (□) ISO-1; (▪) Fraction 3; (▪) genistein. UVB irradiation-induced cell death decreased after treatment with ISO-1 and Fraction 3 by MTT assay. Results in (A) are expressed as percentage of control and the mean ± S.E. (n = 3). *P < 0.05 vs. control. (B) Effects of Fraction 3 and ISO-1 on UVB-induced ERK1/2, p38 and JNK phosphorylation. HaCaT cells were preincubated with Fraction 3 and ISO-1 for 24 h, followed by UVB irradiation. After incubation, cells were collected and lysates were analyzed by Western blot analysis, (F) Fraction 3; (I) ISO-1.
Mentions: Cell viability was 95–100% when treated with the soy isoflavone extract Fraction 3 (Fraction 3), which contains both the aglycone and acetylglucoside forms of isoflavones. The result indicated that soy isoflavone extract Fraction 3 from soy cake is non-toxic for human keratinocytes. In order to explore the photoprotective effects of Fraction 3 on keratinocytes, cells were treated with Fraction 3 (3, 5, 7.5, 10 μg/mL). In control cells, the cell viability significantly decreased after UVB exposure. However, treatment of cells with Fraction 3 obviously increased the cell viability. With the optimal concentration, 5 μg/mL, the cell viability increased about 12% (compare with the control UVB exposure group). The protective effects had no significant difference between 3 and 7.5 μg/mL, and thus the results suggest that Fraction 3 still had a protective effect at a lower concentration (3 μg/mL). In contrast, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) had no significant protective effects on UVB-induced keratinocyte death at the concentration of 3 μg/mL. Moreover, genistein decreased the cell viability in a dose-dependent manner (Figure 1A). In Western blot analysis, the levels of phosphorylation of ERK1/2, p38 and JNK in cells treated with Fraction 3 (3 and 5 μg/mL) were lower than those treated with ISO-1 (3 and 5 μg/mL; Figure 1B). Therefore, Fraction 3 possessed more protective effects on UVB-induced keratinocyte death than ISO-1 and genistein.

Bottom Line: Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome.In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions.Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

View Article: PubMed Central - PubMed

Affiliation: School of Medicine, Fu Jen Catholic University, Taipei, Taiwan; E-Mail: 054317@mail.fju.edu.tw.

ABSTRACT
Soy isoflavones, found in soybean and soybean products, have been reported to possess many physiological activities such as antioxidant activity, inhibition of cancer cell proliferation, reduction of cardiovascular risk, prevention of osteoporosis and alleviation of postmenopausal syndrome. In our previous study, soy isoflavone extract ISO-1 (containing 12 soy isoflavones) from soybean cake was demonstrated to prevent skin damage caused by UVB exposure. In this study, soy isoflavone extract from soybean cake was further purified and evaluated for the protective effects on UVB-induced damage. The results revealed that Fraction 3, which contains the aglycone group (daidzein, genistein and glycitein) and acetylglucoside group (acetyldaidzin, acetylgenistin and acetylglycitin) of soy isoflavones, could inhibit UVB-induced death of human keratinocytes and reduce the level of desquamation, transepidermal water loss (TEWL), erythema and epidermal thickness in mouse skin. Furthermore, topical application of Fraction 3 increased the activity of catalase and suppressed cyclooxygenase-2 (COX-2) and proliferating cell nuclear antigen (PCNA) expression in mice exposed to UVB. In addition, in comparison with ISO-1 and genistein, the Fraction 3 possessed much greater protective effects on both UVB-induced oxidative stress and keratinocyte death than other fractions. Therefore, the soy isoflavone extract Fraction 3 from soybean cake is a desirable anti-photoaging agent for skin care.

Show MeSH
Related in: MedlinePlus