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Immunolymphoscintigraphy for metastatic sentinel nodes: test of a model.

Chakera AH, Nielsen BS, Madsen J, Romer J, Kristjansen PE, Buch I, Binderup T, Ingvar C, Nalla A, Kjaer A, Hesse B - Int J Mol Imaging (2011)

Bottom Line: To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes.Results and Conclusion.Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.

View Article: PubMed Central - PubMed

Affiliation: Clinic of Plastic Surgery, Breast Surgery and Burns Unit, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark.

ABSTRACT
Aim. To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes. Methods. We selected one of four tumour-specific antibodies against human breast cancer and investigated (1), in immune-deficient (nude) mice with xenograft human breast cancer expressing the antigen if specific binding of the intratumorally injected, radioactively labelled, monoclonal antibody could be scintigraphically visualized, and (2) transportation to and retention in regional lymph nodes of the radioactively labelled antibody after subcutaneous injection in healthy rabbits. Results and Conclusion. Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.

No MeSH data available.


Related in: MedlinePlus

Visualization on gamma camera images of groin lymph nodes 15 min. and 22 hours after injection in rabbits of 99mTc-HIG and 99mTc-nanocolloid, respectively.
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Related In: Results  -  Collection


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fig3: Visualization on gamma camera images of groin lymph nodes 15 min. and 22 hours after injection in rabbits of 99mTc-HIG and 99mTc-nanocolloid, respectively.

Mentions: Transport of both 99mTc-HIG and 99mTc-nanocolloid from the subcutaneous depots towards the inguinal lymph nodes was observed in the gamma camera images (Figure 3). 99mTc-nanocolloid accumulated over hours in the lymph nodes with maximum at 22 hours p.i. Gradually, but fairly consistently, some hepatic uptake was observed. 99mTc-HIG passed more rapidly through the nodes with maximum at 5 hours p.i., and activity gradually increased in the liver and in the spine (Table 2 and Figure 4, liver and spine data not shown).


Immunolymphoscintigraphy for metastatic sentinel nodes: test of a model.

Chakera AH, Nielsen BS, Madsen J, Romer J, Kristjansen PE, Buch I, Binderup T, Ingvar C, Nalla A, Kjaer A, Hesse B - Int J Mol Imaging (2011)

Visualization on gamma camera images of groin lymph nodes 15 min. and 22 hours after injection in rabbits of 99mTc-HIG and 99mTc-nanocolloid, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094924&req=5

fig3: Visualization on gamma camera images of groin lymph nodes 15 min. and 22 hours after injection in rabbits of 99mTc-HIG and 99mTc-nanocolloid, respectively.
Mentions: Transport of both 99mTc-HIG and 99mTc-nanocolloid from the subcutaneous depots towards the inguinal lymph nodes was observed in the gamma camera images (Figure 3). 99mTc-nanocolloid accumulated over hours in the lymph nodes with maximum at 22 hours p.i. Gradually, but fairly consistently, some hepatic uptake was observed. 99mTc-HIG passed more rapidly through the nodes with maximum at 5 hours p.i., and activity gradually increased in the liver and in the spine (Table 2 and Figure 4, liver and spine data not shown).

Bottom Line: To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes.Results and Conclusion.Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.

View Article: PubMed Central - PubMed

Affiliation: Clinic of Plastic Surgery, Breast Surgery and Burns Unit, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark.

ABSTRACT
Aim. To develop a method and obtain proof-of-principle for immunolymphoscintigraphy for identification of metastatic sentinel nodes. Methods. We selected one of four tumour-specific antibodies against human breast cancer and investigated (1), in immune-deficient (nude) mice with xenograft human breast cancer expressing the antigen if specific binding of the intratumorally injected, radioactively labelled, monoclonal antibody could be scintigraphically visualized, and (2) transportation to and retention in regional lymph nodes of the radioactively labelled antibody after subcutaneous injection in healthy rabbits. Results and Conclusion. Our paper suggests the theoretical possibility of a model of dual isotope immuno-lymphoscintigraphy for noninvasive, preoperative, malignant sentinel node imaging.

No MeSH data available.


Related in: MedlinePlus