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Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi.

Salomão K, de Souza EM, Henriques-Pons A, Barbosa HS, de Castro SL - Evid Based Complement Alternat Med (2011)

Bottom Line: These effects were confirmed by flow cytometry analysis.The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity.Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil.

ABSTRACT
The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect on Trypanosoma cruzi trypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets in T. cruzi and its effect on experimental infection of mice. The IC(50)/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL(-1), with no damage to the host cells. In epimastigotes Et-Bra induced alterations in reservosomes, Golgi complex and mitochondrion. These effects were confirmed by flow cytometry analysis. In trypomastigotes, Et-Bra led to the loss of plasma membrane integrity. The in vitro studies indicate that Et-Bra interferes in the functionality of the plasma membrane in trypomastigotes and of reservosomes and mitochondrion in epimastigotes. Acutely infected mice were treated orally with Et-Bra and the parasitemia, mortality and GPT, GOT, CK and urea levels were monitored. The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity. Since Et-Bra was not toxic to the animals, it could be assayed in combination with other drugs. Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation.

No MeSH data available.


Related in: MedlinePlus

Effect of Et-Bra treatment on T. cruzi-infected in mice: (a) parasitemia and cumulative mortality curves of infected mice (Tc) and infected and treated with 300 μg mL−1 Et-Bra by oral route, during 10 consecutive days. (b) The body weight of the four experimental groups at days 0, 9 and 17. Asterisks indicate P < .05 for comparison of groups N and Tc.
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fig7: Effect of Et-Bra treatment on T. cruzi-infected in mice: (a) parasitemia and cumulative mortality curves of infected mice (Tc) and infected and treated with 300 μg mL−1 Et-Bra by oral route, during 10 consecutive days. (b) The body weight of the four experimental groups at days 0, 9 and 17. Asterisks indicate P < .05 for comparison of groups N and Tc.

Mentions: In relation to in vivo experiments, the treatment with 25–300 mg kg−1 Et-Bra body weight for 10 consecutive days led to statistically significant decrease in the mortality in comparison with control group, while no important differences were detected in the parasitemia curve (Table 2 and Figure 7(a)). In relation to body weight, the values of infected and treated groups (Tc-PrX) were similar to those of the group Tc (infected and non-treated), being both significantly lower than that of the two non-infected groups (N and N-PrX) (Figure 7(b)). In all the experimental groups at day 14 the GPT, GOT, urea and CK levels were measured directly in the blood. Comparing groups N and Tc, it was observed that infection by T. cruzi led to a significant increase in the four markers: 4.0× for GPT, 8.8× for GOT, 1.3× for urea and 3.6× for CK. Administration of Et-Bra at 300 mg kg−1 led to similar levels of GPT, GOT, urea and CK between the groups N and N-Pr300, indicating that the treatment of the animals with the extract induced no toxicity, as judged by these parameters, as well as by their general behavior, also no difference was observed between the two infected groups, untreated (Tc) and treated (Tc-Pr300) (Figure 8 and Table 3).


Brazilian Green Propolis: Effects In Vitro and In Vivo on Trypanosoma cruzi.

Salomão K, de Souza EM, Henriques-Pons A, Barbosa HS, de Castro SL - Evid Based Complement Alternat Med (2011)

Effect of Et-Bra treatment on T. cruzi-infected in mice: (a) parasitemia and cumulative mortality curves of infected mice (Tc) and infected and treated with 300 μg mL−1 Et-Bra by oral route, during 10 consecutive days. (b) The body weight of the four experimental groups at days 0, 9 and 17. Asterisks indicate P < .05 for comparison of groups N and Tc.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094871&req=5

fig7: Effect of Et-Bra treatment on T. cruzi-infected in mice: (a) parasitemia and cumulative mortality curves of infected mice (Tc) and infected and treated with 300 μg mL−1 Et-Bra by oral route, during 10 consecutive days. (b) The body weight of the four experimental groups at days 0, 9 and 17. Asterisks indicate P < .05 for comparison of groups N and Tc.
Mentions: In relation to in vivo experiments, the treatment with 25–300 mg kg−1 Et-Bra body weight for 10 consecutive days led to statistically significant decrease in the mortality in comparison with control group, while no important differences were detected in the parasitemia curve (Table 2 and Figure 7(a)). In relation to body weight, the values of infected and treated groups (Tc-PrX) were similar to those of the group Tc (infected and non-treated), being both significantly lower than that of the two non-infected groups (N and N-PrX) (Figure 7(b)). In all the experimental groups at day 14 the GPT, GOT, urea and CK levels were measured directly in the blood. Comparing groups N and Tc, it was observed that infection by T. cruzi led to a significant increase in the four markers: 4.0× for GPT, 8.8× for GOT, 1.3× for urea and 3.6× for CK. Administration of Et-Bra at 300 mg kg−1 led to similar levels of GPT, GOT, urea and CK between the groups N and N-Pr300, indicating that the treatment of the animals with the extract induced no toxicity, as judged by these parameters, as well as by their general behavior, also no difference was observed between the two infected groups, untreated (Tc) and treated (Tc-Pr300) (Figure 8 and Table 3).

Bottom Line: These effects were confirmed by flow cytometry analysis.The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity.Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation.

View Article: PubMed Central - PubMed

Affiliation: Laboratório de Biologia Celular, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil.

ABSTRACT
The composition of a Brazilian green propolis ethanolic extract (Et-Bra) and its effect on Trypanosoma cruzi trypomastigotes and other pathogenic microorganisms have already been reported. Here, we further investigated Et-Bra targets in T. cruzi and its effect on experimental infection of mice. The IC(50)/4 days for inhibition of amastigote proliferation was 8.5 ± 1.8 μg mL(-1), with no damage to the host cells. In epimastigotes Et-Bra induced alterations in reservosomes, Golgi complex and mitochondrion. These effects were confirmed by flow cytometry analysis. In trypomastigotes, Et-Bra led to the loss of plasma membrane integrity. The in vitro studies indicate that Et-Bra interferes in the functionality of the plasma membrane in trypomastigotes and of reservosomes and mitochondrion in epimastigotes. Acutely infected mice were treated orally with Et-Bra and the parasitemia, mortality and GPT, GOT, CK and urea levels were monitored. The extract (25-300 mg kg(-1) body weight/day for 10 days) reduced the parasitemia, although not at significant levels; increased the survival of the animals and did not induce any hepatic, muscular lesion or renal toxicity. Since Et-Bra was not toxic to the animals, it could be assayed in combination with other drugs. Et-Bra could be a potential metacyclogenesis blocker, considering its effect on reservosomes, which are an important energy source during parasite differentiation.

No MeSH data available.


Related in: MedlinePlus