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Brazilian green propolis inhibits inflammatory angiogenesis in a murine sponge model.

de Moura SA, Ferreira MA, Andrade SP, Reis ML, Noviello Mde L, Cara DC - Evid Based Complement Alternat Med (2011)

Bottom Line: The levels of vascular endothelial growth factor (VEGF) increased progressively in the treated group but decreased after Day 10 in the control group.Neutrophil accumulation was unaffected by propolis, but NAG activity was reduced by the treatment at Day 14.Our results indicate that the anti-inflammatory/anti-angiogenic effects of propolis are associated with cytokine modulation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.

ABSTRACT
Angiogenesis and inflammation are persistent features of several pathological conditions. Propolis, a sticky material that honeybees collect from living plants, has been reported to have multiple biological effects including anti-inflammatory and anti-neoplasic activities. Here, we investigated the effects of water extract of green propolis (WEP) on angiogenesis, inflammatory cell accumulation and endogenous production of cytokines in sponge implants of mice over a 14-day period. Blood vessel formation as assessed by hemoglobin content and by morphometric analysis of the implants was reduced by WEP (500 mg kg(-1) orally) compared to the untreated group. The levels of vascular endothelial growth factor (VEGF) increased progressively in the treated group but decreased after Day 10 in the control group. Accumulation of neutrophils and macrophages was determined by measuring myeloperoxidase (MPO) and N-acetyl-β-(D)-glucosaminidase (NAG) activities, respectively. Neutrophil accumulation was unaffected by propolis, but NAG activity was reduced by the treatment at Day 14. The levels TGF-β1 intra-implant increased progressively in both groups but were higher (40%) at Day 14 in the control implants. The pro-inflammatory levels of TNF-α peaked at Day 7 in the control implants, and at Day 14 in the propolis-treated group. Our results indicate that the anti-inflammatory/anti-angiogenic effects of propolis are associated with cytokine modulation.

No MeSH data available.


Related in: MedlinePlus

Effects of WEP (500 mg kg−1 day−1) on inflammatory enzyme activities. The level of neutrophil accumulation (measured as myeloperoxidase—MPO activity) is shown in (a). Macrophage accumulation (measured as N-acetyl-β-d-glucosaminidase-NAG activity) is shown in (b). Values are the means (±SEM) from groups of 8–10 animals in each group. *P < .05 versus the control group.
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fig2: Effects of WEP (500 mg kg−1 day−1) on inflammatory enzyme activities. The level of neutrophil accumulation (measured as myeloperoxidase—MPO activity) is shown in (a). Macrophage accumulation (measured as N-acetyl-β-d-glucosaminidase-NAG activity) is shown in (b). Values are the means (±SEM) from groups of 8–10 animals in each group. *P < .05 versus the control group.

Mentions: The inflammatory components of the sponge-induced inflammation were determined by estimating the numbers of leukocytes in the implant through assays for enzyme activities. Neutrophil numbers (as MPO activity) was not affected by propolis treatment at the time points studied; however, macrophage accumulation (as NAG activity) decreased (∼50%) at Day 14 in the treated group compared with the control (Figures 2(a) and 2(b)). The levels of pro-inflammatory cytokine (TNF-α) peaked at Day 7 in the control group and at Day 14 in the propolis-treated group (Figure 3(a)). TGF-β1 levels increased progressively in both types of implant but propolis treatment was able to reduce the amount of this cytokine at Day 14 post-implantation (Figure 3(b)).


Brazilian green propolis inhibits inflammatory angiogenesis in a murine sponge model.

de Moura SA, Ferreira MA, Andrade SP, Reis ML, Noviello Mde L, Cara DC - Evid Based Complement Alternat Med (2011)

Effects of WEP (500 mg kg−1 day−1) on inflammatory enzyme activities. The level of neutrophil accumulation (measured as myeloperoxidase—MPO activity) is shown in (a). Macrophage accumulation (measured as N-acetyl-β-d-glucosaminidase-NAG activity) is shown in (b). Values are the means (±SEM) from groups of 8–10 animals in each group. *P < .05 versus the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094767&req=5

fig2: Effects of WEP (500 mg kg−1 day−1) on inflammatory enzyme activities. The level of neutrophil accumulation (measured as myeloperoxidase—MPO activity) is shown in (a). Macrophage accumulation (measured as N-acetyl-β-d-glucosaminidase-NAG activity) is shown in (b). Values are the means (±SEM) from groups of 8–10 animals in each group. *P < .05 versus the control group.
Mentions: The inflammatory components of the sponge-induced inflammation were determined by estimating the numbers of leukocytes in the implant through assays for enzyme activities. Neutrophil numbers (as MPO activity) was not affected by propolis treatment at the time points studied; however, macrophage accumulation (as NAG activity) decreased (∼50%) at Day 14 in the treated group compared with the control (Figures 2(a) and 2(b)). The levels of pro-inflammatory cytokine (TNF-α) peaked at Day 7 in the control group and at Day 14 in the propolis-treated group (Figure 3(a)). TGF-β1 levels increased progressively in both types of implant but propolis treatment was able to reduce the amount of this cytokine at Day 14 post-implantation (Figure 3(b)).

Bottom Line: The levels of vascular endothelial growth factor (VEGF) increased progressively in the treated group but decreased after Day 10 in the control group.Neutrophil accumulation was unaffected by propolis, but NAG activity was reduced by the treatment at Day 14.Our results indicate that the anti-inflammatory/anti-angiogenic effects of propolis are associated with cytokine modulation.

View Article: PubMed Central - PubMed

Affiliation: Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais (UFMG), Belo Horizonte, Brazil.

ABSTRACT
Angiogenesis and inflammation are persistent features of several pathological conditions. Propolis, a sticky material that honeybees collect from living plants, has been reported to have multiple biological effects including anti-inflammatory and anti-neoplasic activities. Here, we investigated the effects of water extract of green propolis (WEP) on angiogenesis, inflammatory cell accumulation and endogenous production of cytokines in sponge implants of mice over a 14-day period. Blood vessel formation as assessed by hemoglobin content and by morphometric analysis of the implants was reduced by WEP (500 mg kg(-1) orally) compared to the untreated group. The levels of vascular endothelial growth factor (VEGF) increased progressively in the treated group but decreased after Day 10 in the control group. Accumulation of neutrophils and macrophages was determined by measuring myeloperoxidase (MPO) and N-acetyl-β-(D)-glucosaminidase (NAG) activities, respectively. Neutrophil accumulation was unaffected by propolis, but NAG activity was reduced by the treatment at Day 14. The levels TGF-β1 intra-implant increased progressively in both groups but were higher (40%) at Day 14 in the control implants. The pro-inflammatory levels of TNF-α peaked at Day 7 in the control implants, and at Day 14 in the propolis-treated group. Our results indicate that the anti-inflammatory/anti-angiogenic effects of propolis are associated with cytokine modulation.

No MeSH data available.


Related in: MedlinePlus