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Segment-specific generation of Drosophila Capability neuropeptide neurons by multi-faceted Hox cues.

Suska A, Miguel-Aliaga I, Thor S - Dev. Biol. (2011)

Bottom Line: In segments A2-A4 we find that abdominal A is important for the terminal differentiation of Va cell fate.In the A1 segment, Ultrabithorax acts to specify an alternate Va neuron fate.In contrast, in thoracic segments, Antennapedia suppresses the Va cell fate.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, SE-58185, Sweden.

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Expression of Abd-B (A), Abd-A (B), Ubx (C) and Antp (D), in the Va neurons at stage 15. Dimm (blue) and dac-Gal4/UAS-eGFP mark the Va neuron in each segment. (A) Abd-B Is expressed in A5-A8 Va neuron (arrow for A5), but not in more anterior Va neurons (arrowhead for A4). (B) Abd-A is expressed in A2-A7 (arrow for A2), but not in A1 Va neurons (arrowhead). (C) Ubx is expressed in A1-A6 Va neurons and is especially strong in A1 (arrowhead), when compared to A2 (arrow). (D) Antp is expressed T1-A7 Va neurons. (E) Summary of Hox protein expression in the Va neurons along the VNC.
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f0025: Expression of Abd-B (A), Abd-A (B), Ubx (C) and Antp (D), in the Va neurons at stage 15. Dimm (blue) and dac-Gal4/UAS-eGFP mark the Va neuron in each segment. (A) Abd-B Is expressed in A5-A8 Va neuron (arrow for A5), but not in more anterior Va neurons (arrowhead for A4). (B) Abd-A is expressed in A2-A7 (arrow for A2), but not in A1 Va neurons (arrowhead). (C) Ubx is expressed in A1-A6 Va neurons and is especially strong in A1 (arrowhead), when compared to A2 (arrow). (D) Antp is expressed T1-A7 Va neurons. (E) Summary of Hox protein expression in the Va neurons along the VNC.

Mentions: To relate these functional data to the actual expression of these four Hox proteins, we mapped their expression in the Va neurons. Starting from the anterior Va neurons, we find that Antp is the only Hox gene expressed at developmental stage 15 in the T1–T3 neurons. Antp is co-expressed together with the other Hox proteins posteriorly, with the exception of A8 which does not express Antp (Fig. 5D). Ubx is expressed From A1 to A6, and Abd-A in A2 to A7 (Fig. 5B–C). It is noteworthy that A1 Va neurons, which depend upon Ubx for specifying the alternate Va neuron subtype, do not express Abd-A (Fig. 5B, arrowhead). Abd-B is found in A5–A8, but importantly its expression does not extend into A4, in line with the mutant analysis for Abd-B (Fig. 5A, arrowhead).


Segment-specific generation of Drosophila Capability neuropeptide neurons by multi-faceted Hox cues.

Suska A, Miguel-Aliaga I, Thor S - Dev. Biol. (2011)

Expression of Abd-B (A), Abd-A (B), Ubx (C) and Antp (D), in the Va neurons at stage 15. Dimm (blue) and dac-Gal4/UAS-eGFP mark the Va neuron in each segment. (A) Abd-B Is expressed in A5-A8 Va neuron (arrow for A5), but not in more anterior Va neurons (arrowhead for A4). (B) Abd-A is expressed in A2-A7 (arrow for A2), but not in A1 Va neurons (arrowhead). (C) Ubx is expressed in A1-A6 Va neurons and is especially strong in A1 (arrowhead), when compared to A2 (arrow). (D) Antp is expressed T1-A7 Va neurons. (E) Summary of Hox protein expression in the Va neurons along the VNC.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC3094758&req=5

f0025: Expression of Abd-B (A), Abd-A (B), Ubx (C) and Antp (D), in the Va neurons at stage 15. Dimm (blue) and dac-Gal4/UAS-eGFP mark the Va neuron in each segment. (A) Abd-B Is expressed in A5-A8 Va neuron (arrow for A5), but not in more anterior Va neurons (arrowhead for A4). (B) Abd-A is expressed in A2-A7 (arrow for A2), but not in A1 Va neurons (arrowhead). (C) Ubx is expressed in A1-A6 Va neurons and is especially strong in A1 (arrowhead), when compared to A2 (arrow). (D) Antp is expressed T1-A7 Va neurons. (E) Summary of Hox protein expression in the Va neurons along the VNC.
Mentions: To relate these functional data to the actual expression of these four Hox proteins, we mapped their expression in the Va neurons. Starting from the anterior Va neurons, we find that Antp is the only Hox gene expressed at developmental stage 15 in the T1–T3 neurons. Antp is co-expressed together with the other Hox proteins posteriorly, with the exception of A8 which does not express Antp (Fig. 5D). Ubx is expressed From A1 to A6, and Abd-A in A2 to A7 (Fig. 5B–C). It is noteworthy that A1 Va neurons, which depend upon Ubx for specifying the alternate Va neuron subtype, do not express Abd-A (Fig. 5B, arrowhead). Abd-B is found in A5–A8, but importantly its expression does not extend into A4, in line with the mutant analysis for Abd-B (Fig. 5A, arrowhead).

Bottom Line: In segments A2-A4 we find that abdominal A is important for the terminal differentiation of Va cell fate.In the A1 segment, Ultrabithorax acts to specify an alternate Va neuron fate.In contrast, in thoracic segments, Antennapedia suppresses the Va cell fate.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical and Experimental Medicine, Linkoping University, Linkoping, SE-58185, Sweden.

Show MeSH
Related in: MedlinePlus