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Podophyllum hexandrum-Mediated Survival Protection and Restoration of Other Cellular Injuries in Lethally Irradiated Mice.

Sankhwar S, Gupta ML, Gupta V, Verma S, Suri KA, Devi M, Sharma P, Khan EA, Alam MS - Evid Based Complement Alternat Med (2011)

Bottom Line: In the treated irradiated groups, there was a steep reduction in the counts initially, but this phase did not prolong.At later intervals, thymus was found fully regressed.In G-001M pre-treated irradiated groups also, thymocytes decreased till the 5th day but thereafter rejuvenated and within 30 days of treatment the values were close to normal.

View Article: PubMed Central - PubMed

Affiliation: Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. S. K. Majumdar Marg, Timarpur, New Delhi 110054, India.

ABSTRACT
This study aims at the development of a safe and effective formulation to counter the effects of lethal irradiation. The sub-fraction (G-001M), prepared from Podophyllum hexandrum has rendered high degree of survival (>90%) at a dose of 6 mg kg(-1) body weight (intramuscular) in lethally irradiated mice. Therapeutic dose of G-001M, at about 20 times lower concentration than its LD(100), has revealed a DRF of 1.62. Comet assay studies in peripheral blood leukocytes have reflected that, treatment of G-001M before irradiation has significantly reduced DNA tail length (P < .001) and DNA damage score (P < .001), as compared to radiation-only group. Spleen cell counts in irradiated animals had declined drastically at the very first day of exposure, and the fall continued till the 5th day (P < .001). In the treated irradiated groups, there was a steep reduction in the counts initially, but this phase did not prolong. More than 60% decline in thymocytes of irradiated group animals was registered at 5 h of irradiation when compared with controls, and the fall progressed further downwards with the similar pace till 5th day of exposure (P < .001). At later intervals, thymus was found fully regressed. In G-001M pre-treated irradiated groups also, thymocytes decreased till the 5th day but thereafter rejuvenated and within 30 days of treatment the values were close to normal. Current studies have explicitly indicated that, G-001M in very small doses has not only rendered high survivability in lethally irradiated mice, but also protected their cellular DNA, besides supporting fast replenishment of the immune system.

No MeSH data available.


Related in: MedlinePlus

(a) HPLC chromatogram of flavonoids (markers). (b) HPLC chromatogram of P. hexandrum sub-fraction (G-001M) using acetonitrile containing 0.05% of TFA: water containing 0.05% of TFA gradient over a period of 70 min: Column; RP-18 (E. Merck, 5 μm, 4.0 × 250 mm); Flow rate 1.0 ml/min; UV 250 nm.
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fig2: (a) HPLC chromatogram of flavonoids (markers). (b) HPLC chromatogram of P. hexandrum sub-fraction (G-001M) using acetonitrile containing 0.05% of TFA: water containing 0.05% of TFA gradient over a period of 70 min: Column; RP-18 (E. Merck, 5 μm, 4.0 × 250 mm); Flow rate 1.0 ml/min; UV 250 nm.

Mentions: HPLC Tables 1 and 2 indicated presence of lignans namely, Podophyllotoxin, Podophyllotoxin glucoside and Demethyl podophyllotoxin (Table 1, Figures 1(a) and 1(b)) and flavonoids namely, quercetin, quercitrin, rutin and kaempferol (Table 2, Figures 2(a) and 2(b)).


Podophyllum hexandrum-Mediated Survival Protection and Restoration of Other Cellular Injuries in Lethally Irradiated Mice.

Sankhwar S, Gupta ML, Gupta V, Verma S, Suri KA, Devi M, Sharma P, Khan EA, Alam MS - Evid Based Complement Alternat Med (2011)

(a) HPLC chromatogram of flavonoids (markers). (b) HPLC chromatogram of P. hexandrum sub-fraction (G-001M) using acetonitrile containing 0.05% of TFA: water containing 0.05% of TFA gradient over a period of 70 min: Column; RP-18 (E. Merck, 5 μm, 4.0 × 250 mm); Flow rate 1.0 ml/min; UV 250 nm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094713&req=5

fig2: (a) HPLC chromatogram of flavonoids (markers). (b) HPLC chromatogram of P. hexandrum sub-fraction (G-001M) using acetonitrile containing 0.05% of TFA: water containing 0.05% of TFA gradient over a period of 70 min: Column; RP-18 (E. Merck, 5 μm, 4.0 × 250 mm); Flow rate 1.0 ml/min; UV 250 nm.
Mentions: HPLC Tables 1 and 2 indicated presence of lignans namely, Podophyllotoxin, Podophyllotoxin glucoside and Demethyl podophyllotoxin (Table 1, Figures 1(a) and 1(b)) and flavonoids namely, quercetin, quercitrin, rutin and kaempferol (Table 2, Figures 2(a) and 2(b)).

Bottom Line: In the treated irradiated groups, there was a steep reduction in the counts initially, but this phase did not prolong.At later intervals, thymus was found fully regressed.In G-001M pre-treated irradiated groups also, thymocytes decreased till the 5th day but thereafter rejuvenated and within 30 days of treatment the values were close to normal.

View Article: PubMed Central - PubMed

Affiliation: Division of Radiation Biosciences, Institute of Nuclear Medicine and Allied Sciences, Brig. S. K. Majumdar Marg, Timarpur, New Delhi 110054, India.

ABSTRACT
This study aims at the development of a safe and effective formulation to counter the effects of lethal irradiation. The sub-fraction (G-001M), prepared from Podophyllum hexandrum has rendered high degree of survival (>90%) at a dose of 6 mg kg(-1) body weight (intramuscular) in lethally irradiated mice. Therapeutic dose of G-001M, at about 20 times lower concentration than its LD(100), has revealed a DRF of 1.62. Comet assay studies in peripheral blood leukocytes have reflected that, treatment of G-001M before irradiation has significantly reduced DNA tail length (P < .001) and DNA damage score (P < .001), as compared to radiation-only group. Spleen cell counts in irradiated animals had declined drastically at the very first day of exposure, and the fall continued till the 5th day (P < .001). In the treated irradiated groups, there was a steep reduction in the counts initially, but this phase did not prolong. More than 60% decline in thymocytes of irradiated group animals was registered at 5 h of irradiation when compared with controls, and the fall progressed further downwards with the similar pace till 5th day of exposure (P < .001). At later intervals, thymus was found fully regressed. In G-001M pre-treated irradiated groups also, thymocytes decreased till the 5th day but thereafter rejuvenated and within 30 days of treatment the values were close to normal. Current studies have explicitly indicated that, G-001M in very small doses has not only rendered high survivability in lethally irradiated mice, but also protected their cellular DNA, besides supporting fast replenishment of the immune system.

No MeSH data available.


Related in: MedlinePlus