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Antidiabetic Activities of Abutilon indicum (L.) Sweet Are Mediated by Enhancement of Adipocyte Differentiation and Activation of the GLUT1 Promoter.

Krisanapun C, Lee SH, Peungvicha P, Temsiririrkkul R, Baek SJ - Evid Based Complement Alternat Med (2011)

Bottom Line: To measure glucose uptake enhanced by insulin-like activity, we used rat diaphragm incubated with various concentrations of the crude extract and found that the extract enhances glucose consumption in the incubated solution.Our data also indicate that the crude extract and the fractions (water and butanol) did not affect the activity of kinases involved in Akt and GSK-3β pathways; however, the reporter assay showed that the crude extract could activate glucose transporter 1 (GLUT1) promoter activity.These results suggest that the extract from A. indicum L. may be beneficial for reducing insulin resistance through its potency in regulating adipocyte differentiation through PPARγ agonist activity, and increasing glucose utilization via GLUT1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.

ABSTRACT
Abutilon indicum (L.) Sweet is an Asian phytomedicine traditionally used to treat several disorders, including diabetes mellitus. However, molecular mechanisms supporting the antidiabetic effect of A. indicum L. remain unknown. The aim of this study was to evaluate whether extract of A. indicum L. improves insulin sensitivity. First, we observed the antidiabetic activity of aqueous extract of the entire plant (leaves, twigs and roots) of A. indicum L. on postprandial plasma glucose in diabetic rats. The subsequent experiments revealed that butanol fractions of the extract bind to PPARγ and activate 3T3-L1 differentiation. To measure glucose uptake enhanced by insulin-like activity, we used rat diaphragm incubated with various concentrations of the crude extract and found that the extract enhances glucose consumption in the incubated solution. Our data also indicate that the crude extract and the fractions (water and butanol) did not affect the activity of kinases involved in Akt and GSK-3β pathways; however, the reporter assay showed that the crude extract could activate glucose transporter 1 (GLUT1) promoter activity. These results suggest that the extract from A. indicum L. may be beneficial for reducing insulin resistance through its potency in regulating adipocyte differentiation through PPARγ agonist activity, and increasing glucose utilization via GLUT1.

No MeSH data available.


Related in: MedlinePlus

The effect of crude extract of A. indicum on 2 h postprandial plasma glucose in STZ-induced diabetic rats. The results show the mean ± SEM, (n = 6–9). Asterisks represents statistical significance (P < .05) compared with control at each day. Double asterisk represent statistical significance (P < .05) both compared with control at each day and day 0 within the group.
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fig1: The effect of crude extract of A. indicum on 2 h postprandial plasma glucose in STZ-induced diabetic rats. The results show the mean ± SEM, (n = 6–9). Asterisks represents statistical significance (P < .05) compared with control at each day. Double asterisk represent statistical significance (P < .05) both compared with control at each day and day 0 within the group.

Mentions: As shown in Figure 1, administration of A. indicum crude extract significantly lowered (P < .05) 2 h postprandial plasma glucose concentrations of treated groups in comparison to those of control STZ-diabetic rats at the corresponding time. A similar significant decrease was also observed in rats treated with glibenclamide. However, prior to treatment administration (day 0), basal plasma glucose concentration did not significantly differ between groups.


Antidiabetic Activities of Abutilon indicum (L.) Sweet Are Mediated by Enhancement of Adipocyte Differentiation and Activation of the GLUT1 Promoter.

Krisanapun C, Lee SH, Peungvicha P, Temsiririrkkul R, Baek SJ - Evid Based Complement Alternat Med (2011)

The effect of crude extract of A. indicum on 2 h postprandial plasma glucose in STZ-induced diabetic rats. The results show the mean ± SEM, (n = 6–9). Asterisks represents statistical significance (P < .05) compared with control at each day. Double asterisk represent statistical significance (P < .05) both compared with control at each day and day 0 within the group.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094712&req=5

fig1: The effect of crude extract of A. indicum on 2 h postprandial plasma glucose in STZ-induced diabetic rats. The results show the mean ± SEM, (n = 6–9). Asterisks represents statistical significance (P < .05) compared with control at each day. Double asterisk represent statistical significance (P < .05) both compared with control at each day and day 0 within the group.
Mentions: As shown in Figure 1, administration of A. indicum crude extract significantly lowered (P < .05) 2 h postprandial plasma glucose concentrations of treated groups in comparison to those of control STZ-diabetic rats at the corresponding time. A similar significant decrease was also observed in rats treated with glibenclamide. However, prior to treatment administration (day 0), basal plasma glucose concentration did not significantly differ between groups.

Bottom Line: To measure glucose uptake enhanced by insulin-like activity, we used rat diaphragm incubated with various concentrations of the crude extract and found that the extract enhances glucose consumption in the incubated solution.Our data also indicate that the crude extract and the fractions (water and butanol) did not affect the activity of kinases involved in Akt and GSK-3β pathways; however, the reporter assay showed that the crude extract could activate glucose transporter 1 (GLUT1) promoter activity.These results suggest that the extract from A. indicum L. may be beneficial for reducing insulin resistance through its potency in regulating adipocyte differentiation through PPARγ agonist activity, and increasing glucose utilization via GLUT1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathobiology, College of Veterinary Medicine, University of Tennessee, 2407 River Drive, Knoxville, TN 37996, USA.

ABSTRACT
Abutilon indicum (L.) Sweet is an Asian phytomedicine traditionally used to treat several disorders, including diabetes mellitus. However, molecular mechanisms supporting the antidiabetic effect of A. indicum L. remain unknown. The aim of this study was to evaluate whether extract of A. indicum L. improves insulin sensitivity. First, we observed the antidiabetic activity of aqueous extract of the entire plant (leaves, twigs and roots) of A. indicum L. on postprandial plasma glucose in diabetic rats. The subsequent experiments revealed that butanol fractions of the extract bind to PPARγ and activate 3T3-L1 differentiation. To measure glucose uptake enhanced by insulin-like activity, we used rat diaphragm incubated with various concentrations of the crude extract and found that the extract enhances glucose consumption in the incubated solution. Our data also indicate that the crude extract and the fractions (water and butanol) did not affect the activity of kinases involved in Akt and GSK-3β pathways; however, the reporter assay showed that the crude extract could activate glucose transporter 1 (GLUT1) promoter activity. These results suggest that the extract from A. indicum L. may be beneficial for reducing insulin resistance through its potency in regulating adipocyte differentiation through PPARγ agonist activity, and increasing glucose utilization via GLUT1.

No MeSH data available.


Related in: MedlinePlus