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Analyzing Cytotoxic and Apoptogenic Properties of Scutellaria litwinowii Root Extract on Cancer Cell Lines.

Tayarani-Najaran Z, Emami SA, Asili J, Mirzaei A, Mousavi SH - Evid Based Complement Alternat Med (2011)

Bottom Line: Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions.The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively.Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Iran.

ABSTRACT
The Scutellaria species (Lamiaceae) is used as a source of flavonoids to treat a variety of diseases in traditional medicine. In spite of many reports about the cytotoxic and antitumor effects of some species of this genus, anticancer researches on one of the Iranian species S. litwinowii have not yet been conducted. The cytotoxic properties of total methanol extract of S. litwinowii and its fractions were investigated on different cancer cell lines including AGS, HeLa, MCF-7, PC12 and NIH 3T3. Meanwhile, the role of apoptosis in this toxicity was explored. The cells were cultured in DMEM medium and incubated with different concentrations of herb plant extracts. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1 peak). Scutellaria litwinowii inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions. The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively. Scutellaria litwinowii induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in S. litwinowii toxicity. Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

No MeSH data available.


Related in: MedlinePlus

Dose-dependent growth inhibition of malignant cell lines by CH2Cl2 fraction (5–160 μg ml−1) after 24 h. Viability was quantitated by MTT assay. The IC50 values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg ml−1, respectively. Results are mean ± SD (n = 3). *P < .05, **P < .01 and ***P < .001 compared to control.
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fig4: Dose-dependent growth inhibition of malignant cell lines by CH2Cl2 fraction (5–160 μg ml−1) after 24 h. Viability was quantitated by MTT assay. The IC50 values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg ml−1, respectively. Results are mean ± SD (n = 3). *P < .05, **P < .01 and ***P < .001 compared to control.

Mentions: In order to compare the cytotoxicity of solvent fractions of S. litwinowii against malignant cells, another MTT assay was carried out at different concentrations (5–160 μg ml−1). Among them, the CH2Cl2 fraction was found to be more effective than the other fractions of the plant (Figure 4), whereas the other fractions showed no prominent cytotoxicity on the cell lines tested (Table 1). The CH2Cl2 fraction showed inhibitory effect on the proliferation of malignant but not non-malignant cells indicating a degree of specificity for malignant cell lines. The IC50 values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg ml−1, respectively (Table 1).


Analyzing Cytotoxic and Apoptogenic Properties of Scutellaria litwinowii Root Extract on Cancer Cell Lines.

Tayarani-Najaran Z, Emami SA, Asili J, Mirzaei A, Mousavi SH - Evid Based Complement Alternat Med (2011)

Dose-dependent growth inhibition of malignant cell lines by CH2Cl2 fraction (5–160 μg ml−1) after 24 h. Viability was quantitated by MTT assay. The IC50 values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg ml−1, respectively. Results are mean ± SD (n = 3). *P < .05, **P < .01 and ***P < .001 compared to control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094709&req=5

fig4: Dose-dependent growth inhibition of malignant cell lines by CH2Cl2 fraction (5–160 μg ml−1) after 24 h. Viability was quantitated by MTT assay. The IC50 values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg ml−1, respectively. Results are mean ± SD (n = 3). *P < .05, **P < .01 and ***P < .001 compared to control.
Mentions: In order to compare the cytotoxicity of solvent fractions of S. litwinowii against malignant cells, another MTT assay was carried out at different concentrations (5–160 μg ml−1). Among them, the CH2Cl2 fraction was found to be more effective than the other fractions of the plant (Figure 4), whereas the other fractions showed no prominent cytotoxicity on the cell lines tested (Table 1). The CH2Cl2 fraction showed inhibitory effect on the proliferation of malignant but not non-malignant cells indicating a degree of specificity for malignant cell lines. The IC50 values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg ml−1, respectively (Table 1).

Bottom Line: Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions.The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively.Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Iran.

ABSTRACT
The Scutellaria species (Lamiaceae) is used as a source of flavonoids to treat a variety of diseases in traditional medicine. In spite of many reports about the cytotoxic and antitumor effects of some species of this genus, anticancer researches on one of the Iranian species S. litwinowii have not yet been conducted. The cytotoxic properties of total methanol extract of S. litwinowii and its fractions were investigated on different cancer cell lines including AGS, HeLa, MCF-7, PC12 and NIH 3T3. Meanwhile, the role of apoptosis in this toxicity was explored. The cells were cultured in DMEM medium and incubated with different concentrations of herb plant extracts. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1 peak). Scutellaria litwinowii inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions. The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively. Scutellaria litwinowii induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in S. litwinowii toxicity. Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

No MeSH data available.


Related in: MedlinePlus