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Analyzing Cytotoxic and Apoptogenic Properties of Scutellaria litwinowii Root Extract on Cancer Cell Lines.

Tayarani-Najaran Z, Emami SA, Asili J, Mirzaei A, Mousavi SH - Evid Based Complement Alternat Med (2011)

Bottom Line: Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions.The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively.Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Iran.

ABSTRACT
The Scutellaria species (Lamiaceae) is used as a source of flavonoids to treat a variety of diseases in traditional medicine. In spite of many reports about the cytotoxic and antitumor effects of some species of this genus, anticancer researches on one of the Iranian species S. litwinowii have not yet been conducted. The cytotoxic properties of total methanol extract of S. litwinowii and its fractions were investigated on different cancer cell lines including AGS, HeLa, MCF-7, PC12 and NIH 3T3. Meanwhile, the role of apoptosis in this toxicity was explored. The cells were cultured in DMEM medium and incubated with different concentrations of herb plant extracts. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1 peak). Scutellaria litwinowii inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions. The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively. Scutellaria litwinowii induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in S. litwinowii toxicity. Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

No MeSH data available.


Related in: MedlinePlus

Partitioning scheme using immiscible solvents.
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Related In: Results  -  Collection


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fig1: Partitioning scheme using immiscible solvents.

Mentions: The roots of S. litwinowii were collected from Hosseinabad valley (2100 m height) in Pivejan, a village at 65 km south-west of Mashhad, Khorasan Razavi province Iran. The plant was identified by Mr M.R. Joharchi, in Ferdowsi University of Mashhad Herbarium (FUMH). Voucher specimen was deposited in herbarium of faculty of pharmacy, University of Mashhad Medical Sciences. Dry powdered roots (100 g) of S. litwinowii were extracted with methanol (4 × 0.5 l) and were then concentrated at 50°C under reduced pressure to dryness. The concentrated extract was then extracted with an equal volume of n-hexane, three times, to give a fraction containing non-polar compounds, such as lipids. The process is referred to as defatting. Then the solution was successively partitioned between CH2Cl2, ethylacetate (EtOAc) and n-butanol (n-BuOH) and finally water. The isolated fractions were also dried. A partitioning scheme of the methanol extract of S. litwinowii is presented in Figure 1 [19].


Analyzing Cytotoxic and Apoptogenic Properties of Scutellaria litwinowii Root Extract on Cancer Cell Lines.

Tayarani-Najaran Z, Emami SA, Asili J, Mirzaei A, Mousavi SH - Evid Based Complement Alternat Med (2011)

Partitioning scheme using immiscible solvents.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094709&req=5

fig1: Partitioning scheme using immiscible solvents.
Mentions: The roots of S. litwinowii were collected from Hosseinabad valley (2100 m height) in Pivejan, a village at 65 km south-west of Mashhad, Khorasan Razavi province Iran. The plant was identified by Mr M.R. Joharchi, in Ferdowsi University of Mashhad Herbarium (FUMH). Voucher specimen was deposited in herbarium of faculty of pharmacy, University of Mashhad Medical Sciences. Dry powdered roots (100 g) of S. litwinowii were extracted with methanol (4 × 0.5 l) and were then concentrated at 50°C under reduced pressure to dryness. The concentrated extract was then extracted with an equal volume of n-hexane, three times, to give a fraction containing non-polar compounds, such as lipids. The process is referred to as defatting. Then the solution was successively partitioned between CH2Cl2, ethylacetate (EtOAc) and n-butanol (n-BuOH) and finally water. The isolated fractions were also dried. A partitioning scheme of the methanol extract of S. litwinowii is presented in Figure 1 [19].

Bottom Line: Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions.The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively.Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Iran.

ABSTRACT
The Scutellaria species (Lamiaceae) is used as a source of flavonoids to treat a variety of diseases in traditional medicine. In spite of many reports about the cytotoxic and antitumor effects of some species of this genus, anticancer researches on one of the Iranian species S. litwinowii have not yet been conducted. The cytotoxic properties of total methanol extract of S. litwinowii and its fractions were investigated on different cancer cell lines including AGS, HeLa, MCF-7, PC12 and NIH 3T3. Meanwhile, the role of apoptosis in this toxicity was explored. The cells were cultured in DMEM medium and incubated with different concentrations of herb plant extracts. Cell viability was quantitated by MTT assay. Apoptotic cells were determined using propidium iodide staining of DNA fragmentation by flow cytometry (sub-G1 peak). Scutellaria litwinowii inhibited the growth of malignant cells in a dose-dependent manner. Among solvent fractions of S. litwinowii, the methylene chloride fraction was found to be more toxic compared to other fractions. The IC(50) values of this fraction against AGS, HeLa, MCF-7 and PC12 cell lines after 24 h were determined, 121.2 ± 3.1, 40.9 ± 2.5, 115.9 ± 3.5 and 64.5 ± 3.4 μg/ml, respectively. Scutellaria litwinowii induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in S. litwinowii toxicity. Scutellaria litwinowii exerts cytotoxic and proapototic effects in a variety of malignant cell lines and could be considered as a potential chemotherapeutic agent in cancer treatment.

No MeSH data available.


Related in: MedlinePlus