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Electroacupuncture Delays Hypertension Development through Enhancing NO/NOS Activity in Spontaneously Hypertensive Rats.

Hwang HS, Kim YS, Ryu YH, Lee JE, Lee YS, Yang EJ, Choi SM, Lee MS - Evid Based Complement Alternat Med (2011)

Bottom Line: EA was applied to the acupuncture point Governor Vessel 20 (GV20) or to a non-acupuncture point in the tail twice weekly for 3 weeks under anesthesia.Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were lower after 3 weeks of GV20 treatment than EA at non-acupuncture point and no treatment control in SHR. nNOS expression by EA was significantly different between both WKY and no treatment SHR control, and EA at GV20 in SHR. eNOS expression was significantly high in EA at GV 20 compared with no treatment control.In conclusion, EA could attenuate the blood pressure elevation of SHR, along with enhancing NO/NOS activity in the mesenteric artery in SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong-gu, Daejeon 305-811, Republic of Korea.

ABSTRACT
Using spontaneously hypertensive rats (SHR), this study investigated whether electroacupuncture (EA) could reduce early stage hypertension by examining nitric oxide (NO) levels in plasma and nitric oxide synthase (NOS) levels in the mesenteric resistance artery. EA was applied to the acupuncture point Governor Vessel 20 (GV20) or to a non-acupuncture point in the tail twice weekly for 3 weeks under anesthesia. In conscious SHR and normotensive Wistar Kyoto (WKY) rats, blood pressure was determined the day after EA treatment by the tail-cuff method. We measured plasma NO concentration, and evaluated endothelial NO syntheses (eNOS) and neuronal NOS (nNOS) protein expression in the mesenteric artery. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were lower after 3 weeks of GV20 treatment than EA at non-acupuncture point and no treatment control in SHR. nNOS expression by EA was significantly different between both WKY and no treatment SHR control, and EA at GV20 in SHR. eNOS expression was significantly high in EA at GV 20 compared with no treatment control. In conclusion, EA could attenuate the blood pressure elevation of SHR, along with enhancing NO/NOS activity in the mesenteric artery in SHR.

No MeSH data available.


Related in: MedlinePlus

(a) SBP in SHR that were anesthetized for the same period of EA stimulation (SHR+ANES), in Governor Vessel 20 (GV20) treated rats (SHR-GV20), or in non-acupoint treated rats (SHR-Tail) from 5 to 12 weeks. (b) Diastolic blood pressure in SHR (SHR+ANES), GV20 (SHR-GV20) or tail acupuncture groups (SHR-Tail) from 5 to 12 weeks. Results are mean ± SEM for six rats in each group. *P < .05, compared with SHR+ANES. The arrows show the EA treatment for 3 weeks (6–8 weeks).
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fig1: (a) SBP in SHR that were anesthetized for the same period of EA stimulation (SHR+ANES), in Governor Vessel 20 (GV20) treated rats (SHR-GV20), or in non-acupoint treated rats (SHR-Tail) from 5 to 12 weeks. (b) Diastolic blood pressure in SHR (SHR+ANES), GV20 (SHR-GV20) or tail acupuncture groups (SHR-Tail) from 5 to 12 weeks. Results are mean ± SEM for six rats in each group. *P < .05, compared with SHR+ANES. The arrows show the EA treatment for 3 weeks (6–8 weeks).

Mentions: The SBP of 8-week-old SHR was higher than that of the age-matched WKY as shown in Table 1. And the SBP of 4-week and 12-week-old SHR were higher than those of the age matched WKY as listed in Table 1. EA significantly attenuated BP in SHR rats (Figure 1), but not to levels of age-matched untreated WKY as shown in Table 1. SBP at 8 weeks increased progressively to 195.38 ± 2.34 and 193.02 ± 2.97 mmHg in SHR and non-acupuncture point treated group, respectively, while GV20 treatment reduced SBP to 182.2 ± 2.87 mmHg in SHR at the same age. DBP at 8 weeks increased progressively to 169.79 ± 2.5 and 167.21 ± 1.26 mmHg in SHR and non-acupuncture point treated group, respectively, while GV20 treatment reduced DBP to 154.1 ± 4.61 mmHg in SHR at the same age (Figure 1(b)). EA significantly reduced BP in SHR rats (Figure 1), but not to levels of age-matched untreated WKY as listed in Table 1.


Electroacupuncture Delays Hypertension Development through Enhancing NO/NOS Activity in Spontaneously Hypertensive Rats.

Hwang HS, Kim YS, Ryu YH, Lee JE, Lee YS, Yang EJ, Choi SM, Lee MS - Evid Based Complement Alternat Med (2011)

(a) SBP in SHR that were anesthetized for the same period of EA stimulation (SHR+ANES), in Governor Vessel 20 (GV20) treated rats (SHR-GV20), or in non-acupoint treated rats (SHR-Tail) from 5 to 12 weeks. (b) Diastolic blood pressure in SHR (SHR+ANES), GV20 (SHR-GV20) or tail acupuncture groups (SHR-Tail) from 5 to 12 weeks. Results are mean ± SEM for six rats in each group. *P < .05, compared with SHR+ANES. The arrows show the EA treatment for 3 weeks (6–8 weeks).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig1: (a) SBP in SHR that were anesthetized for the same period of EA stimulation (SHR+ANES), in Governor Vessel 20 (GV20) treated rats (SHR-GV20), or in non-acupoint treated rats (SHR-Tail) from 5 to 12 weeks. (b) Diastolic blood pressure in SHR (SHR+ANES), GV20 (SHR-GV20) or tail acupuncture groups (SHR-Tail) from 5 to 12 weeks. Results are mean ± SEM for six rats in each group. *P < .05, compared with SHR+ANES. The arrows show the EA treatment for 3 weeks (6–8 weeks).
Mentions: The SBP of 8-week-old SHR was higher than that of the age-matched WKY as shown in Table 1. And the SBP of 4-week and 12-week-old SHR were higher than those of the age matched WKY as listed in Table 1. EA significantly attenuated BP in SHR rats (Figure 1), but not to levels of age-matched untreated WKY as shown in Table 1. SBP at 8 weeks increased progressively to 195.38 ± 2.34 and 193.02 ± 2.97 mmHg in SHR and non-acupuncture point treated group, respectively, while GV20 treatment reduced SBP to 182.2 ± 2.87 mmHg in SHR at the same age. DBP at 8 weeks increased progressively to 169.79 ± 2.5 and 167.21 ± 1.26 mmHg in SHR and non-acupuncture point treated group, respectively, while GV20 treatment reduced DBP to 154.1 ± 4.61 mmHg in SHR at the same age (Figure 1(b)). EA significantly reduced BP in SHR rats (Figure 1), but not to levels of age-matched untreated WKY as listed in Table 1.

Bottom Line: EA was applied to the acupuncture point Governor Vessel 20 (GV20) or to a non-acupuncture point in the tail twice weekly for 3 weeks under anesthesia.Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were lower after 3 weeks of GV20 treatment than EA at non-acupuncture point and no treatment control in SHR. nNOS expression by EA was significantly different between both WKY and no treatment SHR control, and EA at GV20 in SHR. eNOS expression was significantly high in EA at GV 20 compared with no treatment control.In conclusion, EA could attenuate the blood pressure elevation of SHR, along with enhancing NO/NOS activity in the mesenteric artery in SHR.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Research, Korea Institute of Oriental Medicine, 461-24, Jeonmin-dong, Yuseong-gu, Daejeon 305-811, Republic of Korea.

ABSTRACT
Using spontaneously hypertensive rats (SHR), this study investigated whether electroacupuncture (EA) could reduce early stage hypertension by examining nitric oxide (NO) levels in plasma and nitric oxide synthase (NOS) levels in the mesenteric resistance artery. EA was applied to the acupuncture point Governor Vessel 20 (GV20) or to a non-acupuncture point in the tail twice weekly for 3 weeks under anesthesia. In conscious SHR and normotensive Wistar Kyoto (WKY) rats, blood pressure was determined the day after EA treatment by the tail-cuff method. We measured plasma NO concentration, and evaluated endothelial NO syntheses (eNOS) and neuronal NOS (nNOS) protein expression in the mesenteric artery. Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were lower after 3 weeks of GV20 treatment than EA at non-acupuncture point and no treatment control in SHR. nNOS expression by EA was significantly different between both WKY and no treatment SHR control, and EA at GV20 in SHR. eNOS expression was significantly high in EA at GV 20 compared with no treatment control. In conclusion, EA could attenuate the blood pressure elevation of SHR, along with enhancing NO/NOS activity in the mesenteric artery in SHR.

No MeSH data available.


Related in: MedlinePlus