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Paeoniae alba Radix Promotes Peripheral Nerve Regeneration.

Huang KS, Lin JG, Lee HC, Tsai FJ, Bau DT, Huang CY, Yao CH, Chen YS - Evid Based Complement Alternat Med (2011)

Bottom Line: In the in vivo study, silicone rubber chambers filled with the PR water extract were used to bridge a 10-mm sciatic nerve defect in rats.At the conclusion of 8 weeks, regenerated nerves in the PR groups, especially at 1.25 mg ml(-1) had a higher rate of successful regeneration across the wide gap, relatively larger mean values of total nerve area, myelinated axon count and blood vessel number, and a significantly larger nerve conductive velocity compared to the control group (P  <  .05).These results suggest that the PR extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of injured peripheral nerve.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Chinese Medical Science, China Medical University, Taiwan.

ABSTRACT
The present study provides in vitro and in vivo evaluation of Paeoniae alba Radix (PR) on peripheral nerve regeneration. In the in vitro study, we found the PR caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells as well as their expression of growth associated protein 43 and synapsin I. In the in vivo study, silicone rubber chambers filled with the PR water extract were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, regenerated nerves in the PR groups, especially at 1.25 mg ml(-1) had a higher rate of successful regeneration across the wide gap, relatively larger mean values of total nerve area, myelinated axon count and blood vessel number, and a significantly larger nerve conductive velocity compared to the control group (P  <  .05). These results suggest that the PR extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of injured peripheral nerve.

No MeSH data available.


Related in: MedlinePlus

Effects of PR extract on the morphology of PC12 cells. The cells were treated for 72 h without PR extract in the absence of NGF (a), without PR extract in the presence of NGF (b), with PR extract in the presence of NGF ((c) PR at 1.25 μg ml−1; (d) PR at 12.5 μg ml−1; (e) PR at 125 μg ml−1). It was noted that the PR could stimulate the NGF-induced neurite outgrowths (arrows) from PC12 cells. Scale bars = 100 μm.
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fig1: Effects of PR extract on the morphology of PC12 cells. The cells were treated for 72 h without PR extract in the absence of NGF (a), without PR extract in the presence of NGF (b), with PR extract in the presence of NGF ((c) PR at 1.25 μg ml−1; (d) PR at 12.5 μg ml−1; (e) PR at 125 μg ml−1). It was noted that the PR could stimulate the NGF-induced neurite outgrowths (arrows) from PC12 cells. Scale bars = 100 μm.

Mentions: Under the microscope, almost no PC12 cells exhibited neurite outgrowths of larger than one cell diameter when cultured in the absence of NGF (Figure 1(a)). In comparison, NGF stimulated a few neurite outgrowths from PC12 cells (Figure 1(b)). Additionally, PC12 cells exposed to PR with NGF all formed long neurites, which extended to the neighboring cells of distances even more than three cell diameters (Figures 1(c)–1(e)). These results are supported by quantitative assays that reveal the PR significantly potentiated NGF-induced neurite outgrowth from PC12 cells, especially those in the group with 125 μg ml−1 of PR at P < .05 (Figure 2). In the western blot analysis, the group treated with PR could dramatically increase the expression of GAP-43 and synapsin I in NGF-treated PC12 cells (Figure 3). The enhanced percentage of PC12 cells showed dose dependence after co-treatment of PR at 1.25–125 μg ml−1 with 50 ng ml−1 of NGF.


Paeoniae alba Radix Promotes Peripheral Nerve Regeneration.

Huang KS, Lin JG, Lee HC, Tsai FJ, Bau DT, Huang CY, Yao CH, Chen YS - Evid Based Complement Alternat Med (2011)

Effects of PR extract on the morphology of PC12 cells. The cells were treated for 72 h without PR extract in the absence of NGF (a), without PR extract in the presence of NGF (b), with PR extract in the presence of NGF ((c) PR at 1.25 μg ml−1; (d) PR at 12.5 μg ml−1; (e) PR at 125 μg ml−1). It was noted that the PR could stimulate the NGF-induced neurite outgrowths (arrows) from PC12 cells. Scale bars = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094696&req=5

fig1: Effects of PR extract on the morphology of PC12 cells. The cells were treated for 72 h without PR extract in the absence of NGF (a), without PR extract in the presence of NGF (b), with PR extract in the presence of NGF ((c) PR at 1.25 μg ml−1; (d) PR at 12.5 μg ml−1; (e) PR at 125 μg ml−1). It was noted that the PR could stimulate the NGF-induced neurite outgrowths (arrows) from PC12 cells. Scale bars = 100 μm.
Mentions: Under the microscope, almost no PC12 cells exhibited neurite outgrowths of larger than one cell diameter when cultured in the absence of NGF (Figure 1(a)). In comparison, NGF stimulated a few neurite outgrowths from PC12 cells (Figure 1(b)). Additionally, PC12 cells exposed to PR with NGF all formed long neurites, which extended to the neighboring cells of distances even more than three cell diameters (Figures 1(c)–1(e)). These results are supported by quantitative assays that reveal the PR significantly potentiated NGF-induced neurite outgrowth from PC12 cells, especially those in the group with 125 μg ml−1 of PR at P < .05 (Figure 2). In the western blot analysis, the group treated with PR could dramatically increase the expression of GAP-43 and synapsin I in NGF-treated PC12 cells (Figure 3). The enhanced percentage of PC12 cells showed dose dependence after co-treatment of PR at 1.25–125 μg ml−1 with 50 ng ml−1 of NGF.

Bottom Line: In the in vivo study, silicone rubber chambers filled with the PR water extract were used to bridge a 10-mm sciatic nerve defect in rats.At the conclusion of 8 weeks, regenerated nerves in the PR groups, especially at 1.25 mg ml(-1) had a higher rate of successful regeneration across the wide gap, relatively larger mean values of total nerve area, myelinated axon count and blood vessel number, and a significantly larger nerve conductive velocity compared to the control group (P  <  .05).These results suggest that the PR extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of injured peripheral nerve.

View Article: PubMed Central - PubMed

Affiliation: Graduate Institute of Chinese Medical Science, China Medical University, Taiwan.

ABSTRACT
The present study provides in vitro and in vivo evaluation of Paeoniae alba Radix (PR) on peripheral nerve regeneration. In the in vitro study, we found the PR caused a marked enhancement of the nerve growth factor-mediated neurite outgrowth from PC12 cells as well as their expression of growth associated protein 43 and synapsin I. In the in vivo study, silicone rubber chambers filled with the PR water extract were used to bridge a 10-mm sciatic nerve defect in rats. At the conclusion of 8 weeks, regenerated nerves in the PR groups, especially at 1.25 mg ml(-1) had a higher rate of successful regeneration across the wide gap, relatively larger mean values of total nerve area, myelinated axon count and blood vessel number, and a significantly larger nerve conductive velocity compared to the control group (P  <  .05). These results suggest that the PR extract can be a potential nerve growth-promoting factor, being salutary in aiding the growth of injured peripheral nerve.

No MeSH data available.


Related in: MedlinePlus