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Does visual cortex lactate increase following photic stimulation in migraine without aura patients? A functional (1)H-MRS study.

Reyngoudt H, Paemeleire K, Dierickx A, Descamps B, Vandemaele P, De Deene Y, Achten E - J Headache Pain (2011)

Bottom Line: We did not observe any significant differences in signal integrals, ratios and absolute metabolite concentrations, including Lac, between MwoA patients and controls before PS.Lac also did not increase significantly during and following PS, both for MwoA patients and controls.Our study does, however, argue against a significant switch to non-aerobic glucose metabolism during long-lasting PS of the visual cortex in MwoA patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology and Nuclear Medicine, MR-department (-1K12B), Ghent University Hospital, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium. harmen.reyngoudt@ugent.be

ABSTRACT
Proton magnetic resonance spectroscopy ((1)H-MRS) has been used in a number of studies to assess noninvasively the temporal changes of lactate (Lac) in the activated human brain. Migraine neurobiology involves lack of cortical habituation to repetitive stimuli and a mitochondrial component has been put forward. Our group has recently demonstrated a reduction in the high-energy phosphates adenosine triphosphate (ATP) and phosphocreatine (PCr) in the occipital lobe of migraine without aura (MwoA) patients, at least in a subgroup, in a phosphorus MRS ((31)P-MRS) study. In previous studies, basal Lac levels or photic stimulation (PS)-induced Lac levels were found to be increased in patients with migraine with aura (MwA) and migraine patients with visual symptoms and paraesthesia, paresia and/or dysphasia, respectively. The aim of this study was to perform functional (1)H-MRS at 3 T in 20 MwoA patients and 20 control subjects. Repetitive visual stimulation was applied using MR-compatible goggles with 8 Hz checkerboard stimulation during 12 min. We did not observe any significant differences in signal integrals, ratios and absolute metabolite concentrations, including Lac, between MwoA patients and controls before PS. Lac also did not increase significantly during and following PS, both for MwoA patients and controls. Subtle Lac changes, smaller than the sensitivity threshold (i.e. estimated at 0.1-0.2 μmol/g at 3 T), cannot be detected by MRS. Our study does, however, argue against a significant switch to non-aerobic glucose metabolism during long-lasting PS of the visual cortex in MwoA patients.

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a Sagittal T1-weighted image with a 20-mm cubic VOI localized in the occipital visual cortex. b MR spectrum of a phantom containing NAA (2.01 ppm) and Lac (1.31 ppm). c Stack plot of spectra obtained before (B), during (stimulation 1, S1 and stimulation 2, S2) and following PS (after, A) in controls. d Stack plot of spectra obtained before (B), during (S1 and S2) and following PS (A) in MwoA patients. In vivo metabolites Lac (1.31 ppm), NAA (2.01 ppm), tCr (3.03 ppm) and Cho (3.19 ppm) are marked in the spectra
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Fig2: a Sagittal T1-weighted image with a 20-mm cubic VOI localized in the occipital visual cortex. b MR spectrum of a phantom containing NAA (2.01 ppm) and Lac (1.31 ppm). c Stack plot of spectra obtained before (B), during (stimulation 1, S1 and stimulation 2, S2) and following PS (after, A) in controls. d Stack plot of spectra obtained before (B), during (S1 and S2) and following PS (A) in MwoA patients. In vivo metabolites Lac (1.31 ppm), NAA (2.01 ppm), tCr (3.03 ppm) and Cho (3.19 ppm) are marked in the spectra

Mentions: The volume of interest (VOI) was placed in the primary visual cortex (Brodmann area 17), centered on the calcarine fissure (Fig. 2a), localized on T1-weighted gradient-echo images in three orthogonal planes with a slice thickness of 1 mm, a repetition time (TR) of 1550 ms and an echo time (TE) of 2.37 ms. VOI size was For each subject, 64 water-suppressed spectra (TE = 288 ms, TR = 2,000 ms, 8 averages), as well as 10 water-unsuppressed spectra at different TE (30, 50, 70, 90, 110, 150, 200, 300, 500, 1,000 ms) and a TR of 10,000 ms (1 average) were acquired. The raw data of each acquisition consisted of 1,024 complex-valued data points, at a sampling period of 0.833 ms. The corresponding bandwidth was 1200 Hz. The total duration of the examination was approximately 40 min.Fig. 2


Does visual cortex lactate increase following photic stimulation in migraine without aura patients? A functional (1)H-MRS study.

Reyngoudt H, Paemeleire K, Dierickx A, Descamps B, Vandemaele P, De Deene Y, Achten E - J Headache Pain (2011)

a Sagittal T1-weighted image with a 20-mm cubic VOI localized in the occipital visual cortex. b MR spectrum of a phantom containing NAA (2.01 ppm) and Lac (1.31 ppm). c Stack plot of spectra obtained before (B), during (stimulation 1, S1 and stimulation 2, S2) and following PS (after, A) in controls. d Stack plot of spectra obtained before (B), during (S1 and S2) and following PS (A) in MwoA patients. In vivo metabolites Lac (1.31 ppm), NAA (2.01 ppm), tCr (3.03 ppm) and Cho (3.19 ppm) are marked in the spectra
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094653&req=5

Fig2: a Sagittal T1-weighted image with a 20-mm cubic VOI localized in the occipital visual cortex. b MR spectrum of a phantom containing NAA (2.01 ppm) and Lac (1.31 ppm). c Stack plot of spectra obtained before (B), during (stimulation 1, S1 and stimulation 2, S2) and following PS (after, A) in controls. d Stack plot of spectra obtained before (B), during (S1 and S2) and following PS (A) in MwoA patients. In vivo metabolites Lac (1.31 ppm), NAA (2.01 ppm), tCr (3.03 ppm) and Cho (3.19 ppm) are marked in the spectra
Mentions: The volume of interest (VOI) was placed in the primary visual cortex (Brodmann area 17), centered on the calcarine fissure (Fig. 2a), localized on T1-weighted gradient-echo images in three orthogonal planes with a slice thickness of 1 mm, a repetition time (TR) of 1550 ms and an echo time (TE) of 2.37 ms. VOI size was For each subject, 64 water-suppressed spectra (TE = 288 ms, TR = 2,000 ms, 8 averages), as well as 10 water-unsuppressed spectra at different TE (30, 50, 70, 90, 110, 150, 200, 300, 500, 1,000 ms) and a TR of 10,000 ms (1 average) were acquired. The raw data of each acquisition consisted of 1,024 complex-valued data points, at a sampling period of 0.833 ms. The corresponding bandwidth was 1200 Hz. The total duration of the examination was approximately 40 min.Fig. 2

Bottom Line: We did not observe any significant differences in signal integrals, ratios and absolute metabolite concentrations, including Lac, between MwoA patients and controls before PS.Lac also did not increase significantly during and following PS, both for MwoA patients and controls.Our study does, however, argue against a significant switch to non-aerobic glucose metabolism during long-lasting PS of the visual cortex in MwoA patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology and Nuclear Medicine, MR-department (-1K12B), Ghent University Hospital, Ghent University, De Pintelaan 185, 9000, Ghent, Belgium. harmen.reyngoudt@ugent.be

ABSTRACT
Proton magnetic resonance spectroscopy ((1)H-MRS) has been used in a number of studies to assess noninvasively the temporal changes of lactate (Lac) in the activated human brain. Migraine neurobiology involves lack of cortical habituation to repetitive stimuli and a mitochondrial component has been put forward. Our group has recently demonstrated a reduction in the high-energy phosphates adenosine triphosphate (ATP) and phosphocreatine (PCr) in the occipital lobe of migraine without aura (MwoA) patients, at least in a subgroup, in a phosphorus MRS ((31)P-MRS) study. In previous studies, basal Lac levels or photic stimulation (PS)-induced Lac levels were found to be increased in patients with migraine with aura (MwA) and migraine patients with visual symptoms and paraesthesia, paresia and/or dysphasia, respectively. The aim of this study was to perform functional (1)H-MRS at 3 T in 20 MwoA patients and 20 control subjects. Repetitive visual stimulation was applied using MR-compatible goggles with 8 Hz checkerboard stimulation during 12 min. We did not observe any significant differences in signal integrals, ratios and absolute metabolite concentrations, including Lac, between MwoA patients and controls before PS. Lac also did not increase significantly during and following PS, both for MwoA patients and controls. Subtle Lac changes, smaller than the sensitivity threshold (i.e. estimated at 0.1-0.2 μmol/g at 3 T), cannot be detected by MRS. Our study does, however, argue against a significant switch to non-aerobic glucose metabolism during long-lasting PS of the visual cortex in MwoA patients.

Show MeSH
Related in: MedlinePlus