Limits...
A validated densitometric method for analysis of atorvastatin calcium and metoprolol tartarate as bulk drugs and in combined capsule dosage forms.

Patole S, Khodke A, Potale L, Damle M - J Young Pharm (2011)

Bottom Line: Densitometric scanning was performed at 220 nm using Camag TLC scanner 3.The method was linear in the drug concentrations' range of 500 to 2500 ng/spot for Atorvastatin Calcium, also for Metoprolol Tartarate with correlation coefficient of 0.984 for Atorvastatin Calcium and 0.995 for Metoprolol Tartarate respectively.The retention factor for Atorvastatin Calcium was 0.45 ± 0.04 and for Metoprolol Tartarate was 0.25 ± 0.02.

View Article: PubMed Central - PubMed

Affiliation: Department of Quality Assurance, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune - 411 001.

ABSTRACT
A simple, accurate and precise high-performance thin-layer chromatographic method has been developed for the estimation of Atorvastatin Calcium and Metoprolol Tartarate simultaneously from a capsule dosage form. The method employed Silica gel 60F (254s)precoated plates as stationary phase and a mixture of Chloroform: Methanol: Glacial acetic acid (dil.) :: (9:1.5:0.2 ml %v/v) as mobile phase. Densitometric scanning was performed at 220 nm using Camag TLC scanner 3. The method was linear in the drug concentrations' range of 500 to 2500 ng/spot for Atorvastatin Calcium, also for Metoprolol Tartarate with correlation coefficient of 0.984 for Atorvastatin Calcium and 0.995 for Metoprolol Tartarate respectively. The retention factor for Atorvastatin Calcium was 0.45 ± 0.04 and for Metoprolol Tartarate was 0.25 ± 0.02. The method was validated as per ICH (International Conference on Harmonisation) Guidelines, proving its utility in estimation of Atorvastatin Calcium and Metoprolol Tartarate in combined dosage form.

No MeSH data available.


Related in: MedlinePlus

Representative densitogram (linearity) of atorvastatin calcium and metoprolol tartarate
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3094561&req=5

Figure 0001: Representative densitogram (linearity) of atorvastatin calcium and metoprolol tartarate

Mentions: Four capsules were weighed accurately and powder content was removed. Powder quantities equivalent to 10 mg of Atorvastatin Calcium and 25 mg of Metoprolol Tartarate were weighed separately and transferred to 25-ml volumetric flask and volume was made up. The resultant solution was further sonicated for 10 min. The volume was then made up to 10 ml with same solvent. Each solution was then filtered through whatmann filter paper no.41. From the filtrate, appropriate volumes were spotted to obtain final concentration of 320 ng/spot for Atorvastatin Calcium and 800 ng/spot for Metoprolol Tartarate. Spotting was done in the form of bands and plate was developed up to a distance of 90 mm, using the mobile phase Chloroform: Methanol: Glacial acetic acid (dil.) :: (9:1.5:0.2ml v/v) in normal conditions of temperature and humidity. The peak areas of the spots were measured at 220 nm where both the drugs showed considerable absorbances [Figure 1] and concentrations in the samples were determined from the respective calibration curves. The amount of each drug present per capsule was calculated.Figure 1


A validated densitometric method for analysis of atorvastatin calcium and metoprolol tartarate as bulk drugs and in combined capsule dosage forms.

Patole S, Khodke A, Potale L, Damle M - J Young Pharm (2011)

Representative densitogram (linearity) of atorvastatin calcium and metoprolol tartarate
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094561&req=5

Figure 0001: Representative densitogram (linearity) of atorvastatin calcium and metoprolol tartarate
Mentions: Four capsules were weighed accurately and powder content was removed. Powder quantities equivalent to 10 mg of Atorvastatin Calcium and 25 mg of Metoprolol Tartarate were weighed separately and transferred to 25-ml volumetric flask and volume was made up. The resultant solution was further sonicated for 10 min. The volume was then made up to 10 ml with same solvent. Each solution was then filtered through whatmann filter paper no.41. From the filtrate, appropriate volumes were spotted to obtain final concentration of 320 ng/spot for Atorvastatin Calcium and 800 ng/spot for Metoprolol Tartarate. Spotting was done in the form of bands and plate was developed up to a distance of 90 mm, using the mobile phase Chloroform: Methanol: Glacial acetic acid (dil.) :: (9:1.5:0.2ml v/v) in normal conditions of temperature and humidity. The peak areas of the spots were measured at 220 nm where both the drugs showed considerable absorbances [Figure 1] and concentrations in the samples were determined from the respective calibration curves. The amount of each drug present per capsule was calculated.Figure 1

Bottom Line: Densitometric scanning was performed at 220 nm using Camag TLC scanner 3.The method was linear in the drug concentrations' range of 500 to 2500 ng/spot for Atorvastatin Calcium, also for Metoprolol Tartarate with correlation coefficient of 0.984 for Atorvastatin Calcium and 0.995 for Metoprolol Tartarate respectively.The retention factor for Atorvastatin Calcium was 0.45 ± 0.04 and for Metoprolol Tartarate was 0.25 ± 0.02.

View Article: PubMed Central - PubMed

Affiliation: Department of Quality Assurance, A.I.S.S.M.S. College of Pharmacy, Kennedy Road, Near R.T.O., Pune - 411 001.

ABSTRACT
A simple, accurate and precise high-performance thin-layer chromatographic method has been developed for the estimation of Atorvastatin Calcium and Metoprolol Tartarate simultaneously from a capsule dosage form. The method employed Silica gel 60F (254s)precoated plates as stationary phase and a mixture of Chloroform: Methanol: Glacial acetic acid (dil.) :: (9:1.5:0.2 ml %v/v) as mobile phase. Densitometric scanning was performed at 220 nm using Camag TLC scanner 3. The method was linear in the drug concentrations' range of 500 to 2500 ng/spot for Atorvastatin Calcium, also for Metoprolol Tartarate with correlation coefficient of 0.984 for Atorvastatin Calcium and 0.995 for Metoprolol Tartarate respectively. The retention factor for Atorvastatin Calcium was 0.45 ± 0.04 and for Metoprolol Tartarate was 0.25 ± 0.02. The method was validated as per ICH (International Conference on Harmonisation) Guidelines, proving its utility in estimation of Atorvastatin Calcium and Metoprolol Tartarate in combined dosage form.

No MeSH data available.


Related in: MedlinePlus