Limits...
Antiurolithiatic Activity of Whole-Plant Hydroalcoholic Extract of Pergularia daemia in Rats.

Vyas B, Vyas R, Joshi S, Santani D - J Young Pharm (2011)

Bottom Line: Furthermore, high serum levels of urea nitrogen, creatinine and uric acid were significantly (P < 0.001) reduced by the extract.The results were comparable with the standard drug, cystone (750 mg/kg).The reduction of stone-forming constituents in urine and their decreased kidney retention reduces the solubility product of crystallizing salts such as calcium oxalate and calcium phosphate, which could contribute to the antiurolithiatic property of the extract.

View Article: PubMed Central - PubMed

Affiliation: Maliba Pharmacy College, Gopal Vidyanagar, Tarsadi, Gujarat - 394 350, India.

ABSTRACT
The whole-plant, Pergularia daemia (Family: Asclepediaceae), extract (50% alcohol) was investigated for its antiurolithiatic and diuretic activity. Ethylene glycol (0.75% in water) feeding resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. Alcoholic extract (400 mg/kg) of P. daemia was given orally in curative and preventive regimens over a period of 28 days. Supplementation with extract significantly (P < 0.001) lowered the urinary excretion and kidney retention levels of oxalate, calcium and phosphate. Furthermore, high serum levels of urea nitrogen, creatinine and uric acid were significantly (P < 0.001) reduced by the extract. The results were comparable with the standard drug, cystone (750 mg/kg). The reduction of stone-forming constituents in urine and their decreased kidney retention reduces the solubility product of crystallizing salts such as calcium oxalate and calcium phosphate, which could contribute to the antiurolithiatic property of the extract. The extract exhibited significant diuretic activity at dose of 400 mg/kg body weight as evidenced by increased total urine volume and the urine concentration of Na(+), and K(+). These findings affirm assertions made regarding the effectiveness of the extract of this plant against urinary pathologies in the Indian folk medicine.

No MeSH data available.


Related in: MedlinePlus

Effect of P. daemia extract on lipid peroxidation of kidney in control and experimental animals (All values represent mean ± S.E.M, N = 6, * P < 0.001, compared to control Group I, N = 6, ** P < 0.001, compared to ethylene glycol Group II, One-way analysis of variance (ANOVA) followed by “Dunnett’s test.”)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3094557&req=5

Figure 0004: Effect of P. daemia extract on lipid peroxidation of kidney in control and experimental animals (All values represent mean ± S.E.M, N = 6, * P < 0.001, compared to control Group I, N = 6, ** P < 0.001, compared to ethylene glycol Group II, One-way analysis of variance (ANOVA) followed by “Dunnett’s test.”)

Mentions: In the present study, calculi-treated rats (Group II) showed an increase in malondialdehyde (MDA) content, representing lipid peroxidation, compared with untreated group in kidney. Administration of extract (400 mg/kg) and cystone (750 mg/kg) showed significant inhibition of lipid peroxide level in rat kidney in both the prophylactic and therapeutic groups [Figure 4]. Recent studies show increased urinary excretion of MDA in human calcium-oxalate kidney-stone-formers.[23] In addition, another recent study provided more indication signifying that oxidative stress plays a role in kidney-stone formation in humans.[24] The present results suggest that supplementation with P. daemia extract significantly decreased oxalate-induced kidney lipid peroxidation.


Antiurolithiatic Activity of Whole-Plant Hydroalcoholic Extract of Pergularia daemia in Rats.

Vyas B, Vyas R, Joshi S, Santani D - J Young Pharm (2011)

Effect of P. daemia extract on lipid peroxidation of kidney in control and experimental animals (All values represent mean ± S.E.M, N = 6, * P < 0.001, compared to control Group I, N = 6, ** P < 0.001, compared to ethylene glycol Group II, One-way analysis of variance (ANOVA) followed by “Dunnett’s test.”)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094557&req=5

Figure 0004: Effect of P. daemia extract on lipid peroxidation of kidney in control and experimental animals (All values represent mean ± S.E.M, N = 6, * P < 0.001, compared to control Group I, N = 6, ** P < 0.001, compared to ethylene glycol Group II, One-way analysis of variance (ANOVA) followed by “Dunnett’s test.”)
Mentions: In the present study, calculi-treated rats (Group II) showed an increase in malondialdehyde (MDA) content, representing lipid peroxidation, compared with untreated group in kidney. Administration of extract (400 mg/kg) and cystone (750 mg/kg) showed significant inhibition of lipid peroxide level in rat kidney in both the prophylactic and therapeutic groups [Figure 4]. Recent studies show increased urinary excretion of MDA in human calcium-oxalate kidney-stone-formers.[23] In addition, another recent study provided more indication signifying that oxidative stress plays a role in kidney-stone formation in humans.[24] The present results suggest that supplementation with P. daemia extract significantly decreased oxalate-induced kidney lipid peroxidation.

Bottom Line: Furthermore, high serum levels of urea nitrogen, creatinine and uric acid were significantly (P < 0.001) reduced by the extract.The results were comparable with the standard drug, cystone (750 mg/kg).The reduction of stone-forming constituents in urine and their decreased kidney retention reduces the solubility product of crystallizing salts such as calcium oxalate and calcium phosphate, which could contribute to the antiurolithiatic property of the extract.

View Article: PubMed Central - PubMed

Affiliation: Maliba Pharmacy College, Gopal Vidyanagar, Tarsadi, Gujarat - 394 350, India.

ABSTRACT
The whole-plant, Pergularia daemia (Family: Asclepediaceae), extract (50% alcohol) was investigated for its antiurolithiatic and diuretic activity. Ethylene glycol (0.75% in water) feeding resulted in hyperoxaluria as well as increased renal excretion of calcium and phosphate. Alcoholic extract (400 mg/kg) of P. daemia was given orally in curative and preventive regimens over a period of 28 days. Supplementation with extract significantly (P < 0.001) lowered the urinary excretion and kidney retention levels of oxalate, calcium and phosphate. Furthermore, high serum levels of urea nitrogen, creatinine and uric acid were significantly (P < 0.001) reduced by the extract. The results were comparable with the standard drug, cystone (750 mg/kg). The reduction of stone-forming constituents in urine and their decreased kidney retention reduces the solubility product of crystallizing salts such as calcium oxalate and calcium phosphate, which could contribute to the antiurolithiatic property of the extract. The extract exhibited significant diuretic activity at dose of 400 mg/kg body weight as evidenced by increased total urine volume and the urine concentration of Na(+), and K(+). These findings affirm assertions made regarding the effectiveness of the extract of this plant against urinary pathologies in the Indian folk medicine.

No MeSH data available.


Related in: MedlinePlus