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Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663.

Jian P, Li ZW, Fang TY, Jian W, Zhuan Z, Mei LX, Yan WS, Jian N - J Hematol Oncol (2011)

Bottom Line: We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells.Transfection of LV-miR-663 induced significant HL-60 cell differentiation in vitro.Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, China.

ABSTRACT

Background: Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.

Methods: Using bioinformatics and in vitro experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation.

Results: Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation in vitro.

Conclusions: Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies.

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Related in: MedlinePlus

MicroRNA expression in HL-60 cells during ATRA-induced differentiation. The microRNA profile in HL-60 cells treated with ATRA or 0.1% alcohol was determined using a microRNA microarray as described in the materials and methods (A) significance analysis of microarrays of differentially regulated microRNAs in ATRA treated cells. (B) List of differentially expressed microRNA and their fold expression changes.
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Figure 2: MicroRNA expression in HL-60 cells during ATRA-induced differentiation. The microRNA profile in HL-60 cells treated with ATRA or 0.1% alcohol was determined using a microRNA microarray as described in the materials and methods (A) significance analysis of microarrays of differentially regulated microRNAs in ATRA treated cells. (B) List of differentially expressed microRNA and their fold expression changes.

Mentions: A miRNA microarray identified the expression of several microRNAs significantly changed in HL-60 cells during ATRA-induced differentiation. Significance analysis of microarrays (Figure 2A) was used to identify miRNAs whose expression was altered more than 2 fold in response to treatment with ATRA for 24-72 h, and the differentially expressed microRNAs are listed in Figure 2B.


Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663.

Jian P, Li ZW, Fang TY, Jian W, Zhuan Z, Mei LX, Yan WS, Jian N - J Hematol Oncol (2011)

MicroRNA expression in HL-60 cells during ATRA-induced differentiation. The microRNA profile in HL-60 cells treated with ATRA or 0.1% alcohol was determined using a microRNA microarray as described in the materials and methods (A) significance analysis of microarrays of differentially regulated microRNAs in ATRA treated cells. (B) List of differentially expressed microRNA and their fold expression changes.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094392&req=5

Figure 2: MicroRNA expression in HL-60 cells during ATRA-induced differentiation. The microRNA profile in HL-60 cells treated with ATRA or 0.1% alcohol was determined using a microRNA microarray as described in the materials and methods (A) significance analysis of microarrays of differentially regulated microRNAs in ATRA treated cells. (B) List of differentially expressed microRNA and their fold expression changes.
Mentions: A miRNA microarray identified the expression of several microRNAs significantly changed in HL-60 cells during ATRA-induced differentiation. Significance analysis of microarrays (Figure 2A) was used to identify miRNAs whose expression was altered more than 2 fold in response to treatment with ATRA for 24-72 h, and the differentially expressed microRNAs are listed in Figure 2B.

Bottom Line: We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells.Transfection of LV-miR-663 induced significant HL-60 cell differentiation in vitro.Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Hematology and Oncology, Children's Hospital of Soochow University, Suzhou, China.

ABSTRACT

Background: Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized.

Methods: Using bioinformatics and in vitro experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation.

Results: Six microRNAs were upregulated by ATRA treatment, miR-663, miR-494, miR-145, miR-22, miR-363* and miR-223; and three microRNAs were downregulated, miR-10a, miR-181 and miR-612. Additionally, miR-663 expression was regulated by ATRA. We used a lentivirus (LV) backbone incorporating the spleen focus forming virus (SFFV-F) promoter to drive miR-663 expression, as the CMV (Cytomegalovirus) promoter is ineffective in some lymphocyte cells. Transfection of LV-miR-663 induced significant HL-60 cell differentiation in vitro.

Conclusions: Our results show miR-663 may play an important role in ATRA induced HL-60 cell differentiation. Lentivirus delivery of miR-663 could potentially be used directly as an anticancer treatment in hematological malignancies.

Show MeSH
Related in: MedlinePlus