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Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation.

Wu X, Zhu K, Du X, Chen S, Yang L, Wu J, Liu Q, Li Y - J Hematol Oncol (2011)

Bottom Line: Patients with acute graft-versus-host disease (GVHD) or chronic GVHD had profoundly reduced TRECs levels during the first year post-HSCT.Reconstitution of thymic output function resulted in a period of immunodeficiency, with low or undetectable TRECs after transplantation, although fludarabine-based dose-reduced conditioning regimens were used.Low frequency of BV22-BD1 and BV23-BD1 sjTRECs might be associated with GVHD.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, PR China.

ABSTRACT

Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) leads to a prolonged state of immunodeficiency and requires reconstitution of normal T-cell immunity. Signal joint T-cell receptor excision DNA circles (sjTRECs) are markers of developmental proximity to the thymus that have been used to evaluate thymic function related to T-cell immune reconstitution after HSCT. To assess the proliferative history in different T-cell receptor beta variable region (TRBV) subfamilies of T cells after HSCT, expansion of TRBV subfamily-naive T cells was determined by analysis of a series of TRBV-BD1 sjTRECs.

Methods: sjTRECs levels were detected by real-time quantitative polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMCs) from 43 Chinese acute leukemia patients who underwent allo-HSCT. Twenty-three TRBV-BD1 sjTRECs were amplified by semi-nested PCR. Sixteen age-matched healthy volunteers served as normal controls.

Results: sjTRECs levels were low or undetectable in the first 6 weeks after allo-HSCT and increased after 8 weeks post HSCT; however, sjTRECs levels at week 20 post-HSCT were still less than normal controls. Frequencies of TRBV subfamily sjTRECs in PBMCs from recipients at week 8 post-HSCT (29.17 ± 20.97%) or at week 16 post-HSCT (38.33 ± 9.03%) were significantly lower than those in donors (47.92 ± 13.82%) or recipients at pre-HSCT (45.83 ± 14.03%). However, frequencies of TRBV subfamily sjTRECs in recipients at week 30 post-HSCT (42.71 ± 21.62%) were similar to those in donors and recipients at pre-HSCT. sjTRECs levels in donors had a positive linear correlation with sjTRECs levels in recipients within 8-12 weeks post-HSCT. Patients with acute graft-versus-host disease (GVHD) or chronic GVHD had profoundly reduced TRECs levels during the first year post-HSCT. Frequencies of BV22-BD1 sjTRECs and BV23-BD1 sjTRECs in patients with GVHD were significantly lower than those in recipients at pre-HSCT, and the frequencies of BV22-BD1 sjTRECs in patients with GVHD were significantly lower than those in donors.

Conclusions: Reconstitution of thymic output function resulted in a period of immunodeficiency, with low or undetectable TRECs after transplantation, although fludarabine-based dose-reduced conditioning regimens were used. GVHD could affect reconstitution of thymic output function and reduce sjTRECs levels and frequencies of TRBV-BD1 sjTRECs. Low frequency of BV22-BD1 and BV23-BD1 sjTRECs might be associated with GVHD.

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Frequencies of 23 TRBV-BD1 sjTRECs subfamilies in PBMCs from patients with GVHD, donors, and recipients at pre-HSCT. * P < 0.05, comparing patients with GVHD to recipients at pre HSCT. ** P < 0.05, comparing patients with GVHD to donors.
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Figure 4: Frequencies of 23 TRBV-BD1 sjTRECs subfamilies in PBMCs from patients with GVHD, donors, and recipients at pre-HSCT. * P < 0.05, comparing patients with GVHD to recipients at pre HSCT. ** P < 0.05, comparing patients with GVHD to donors.

Mentions: Comparison of the frequencies of 23 TRBV-BD1 sjTRECs among patients with GVHD, donors, and recipients at pre-HSCT showed that the frequencies of BV22-BD1 sjTRECs and BV23-BD1 sjTRECs in patients with GVHD were significantly lower than those in recipients at pre-HSCT (P = 0.039, 0.012), and the frequencies of BV22-BD1 sjTRECs in patients with GVHD were significantly lower than those in donors (P = 0.003). However, no significant difference was found in the frequencies of other TRBV-BD1 sjTRECs among groups of patients with GVHD and donors and recipients at pre-HSCT (P > 0.05; Figure 4).


Frequency analysis of TRBV subfamily sjTRECs to characterize T-cell reconstitution in acute leukemia patients after allogeneic hematopoietic stem cell transplantation.

Wu X, Zhu K, Du X, Chen S, Yang L, Wu J, Liu Q, Li Y - J Hematol Oncol (2011)

Frequencies of 23 TRBV-BD1 sjTRECs subfamilies in PBMCs from patients with GVHD, donors, and recipients at pre-HSCT. * P < 0.05, comparing patients with GVHD to recipients at pre HSCT. ** P < 0.05, comparing patients with GVHD to donors.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094391&req=5

Figure 4: Frequencies of 23 TRBV-BD1 sjTRECs subfamilies in PBMCs from patients with GVHD, donors, and recipients at pre-HSCT. * P < 0.05, comparing patients with GVHD to recipients at pre HSCT. ** P < 0.05, comparing patients with GVHD to donors.
Mentions: Comparison of the frequencies of 23 TRBV-BD1 sjTRECs among patients with GVHD, donors, and recipients at pre-HSCT showed that the frequencies of BV22-BD1 sjTRECs and BV23-BD1 sjTRECs in patients with GVHD were significantly lower than those in recipients at pre-HSCT (P = 0.039, 0.012), and the frequencies of BV22-BD1 sjTRECs in patients with GVHD were significantly lower than those in donors (P = 0.003). However, no significant difference was found in the frequencies of other TRBV-BD1 sjTRECs among groups of patients with GVHD and donors and recipients at pre-HSCT (P > 0.05; Figure 4).

Bottom Line: Patients with acute graft-versus-host disease (GVHD) or chronic GVHD had profoundly reduced TRECs levels during the first year post-HSCT.Reconstitution of thymic output function resulted in a period of immunodeficiency, with low or undetectable TRECs after transplantation, although fludarabine-based dose-reduced conditioning regimens were used.Low frequency of BV22-BD1 and BV23-BD1 sjTRECs might be associated with GVHD.

View Article: PubMed Central - HTML - PubMed

Affiliation: Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, PR China.

ABSTRACT

Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) leads to a prolonged state of immunodeficiency and requires reconstitution of normal T-cell immunity. Signal joint T-cell receptor excision DNA circles (sjTRECs) are markers of developmental proximity to the thymus that have been used to evaluate thymic function related to T-cell immune reconstitution after HSCT. To assess the proliferative history in different T-cell receptor beta variable region (TRBV) subfamilies of T cells after HSCT, expansion of TRBV subfamily-naive T cells was determined by analysis of a series of TRBV-BD1 sjTRECs.

Methods: sjTRECs levels were detected by real-time quantitative polymerase chain reaction (PCR) in peripheral blood mononuclear cells (PBMCs) from 43 Chinese acute leukemia patients who underwent allo-HSCT. Twenty-three TRBV-BD1 sjTRECs were amplified by semi-nested PCR. Sixteen age-matched healthy volunteers served as normal controls.

Results: sjTRECs levels were low or undetectable in the first 6 weeks after allo-HSCT and increased after 8 weeks post HSCT; however, sjTRECs levels at week 20 post-HSCT were still less than normal controls. Frequencies of TRBV subfamily sjTRECs in PBMCs from recipients at week 8 post-HSCT (29.17 ± 20.97%) or at week 16 post-HSCT (38.33 ± 9.03%) were significantly lower than those in donors (47.92 ± 13.82%) or recipients at pre-HSCT (45.83 ± 14.03%). However, frequencies of TRBV subfamily sjTRECs in recipients at week 30 post-HSCT (42.71 ± 21.62%) were similar to those in donors and recipients at pre-HSCT. sjTRECs levels in donors had a positive linear correlation with sjTRECs levels in recipients within 8-12 weeks post-HSCT. Patients with acute graft-versus-host disease (GVHD) or chronic GVHD had profoundly reduced TRECs levels during the first year post-HSCT. Frequencies of BV22-BD1 sjTRECs and BV23-BD1 sjTRECs in patients with GVHD were significantly lower than those in recipients at pre-HSCT, and the frequencies of BV22-BD1 sjTRECs in patients with GVHD were significantly lower than those in donors.

Conclusions: Reconstitution of thymic output function resulted in a period of immunodeficiency, with low or undetectable TRECs after transplantation, although fludarabine-based dose-reduced conditioning regimens were used. GVHD could affect reconstitution of thymic output function and reduce sjTRECs levels and frequencies of TRBV-BD1 sjTRECs. Low frequency of BV22-BD1 and BV23-BD1 sjTRECs might be associated with GVHD.

Show MeSH
Related in: MedlinePlus