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Gene expression profiles in rat mesenteric lymph nodes upon supplementation with conjugated linoleic acid during gestation and suckling.

Selga E, Pérez-Cano FJ, Franch A, Ramírez-Santana C, Rivero M, Ciudad CJ, Castellote C, Noé V - BMC Genomics (2011)

Bottom Line: However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network.Gene underexpression was confirmed by Real-Time RT-PCR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Spain.

ABSTRACT

Background: Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.

Results: The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip(®) Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR.

Conclusions: Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.

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Related in: MedlinePlus

Venn Diagram of differentially expressed genes. Venn diagrams in GeneSpring GX were used to compare the lists of genes differentially expressed by 2-fold (p < 0.05) in each of the three experimental groups of animals. The diagrams show the number of genes that belong to each of the individual lists, the genes in common between each pair of lists and the genes in common among all three lists (in the center of the representation) for each type of regulation (A: Upregulation and B: Downregulation).
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Figure 2: Venn Diagram of differentially expressed genes. Venn diagrams in GeneSpring GX were used to compare the lists of genes differentially expressed by 2-fold (p < 0.05) in each of the three experimental groups of animals. The diagrams show the number of genes that belong to each of the individual lists, the genes in common between each pair of lists and the genes in common among all three lists (in the center of the representation) for each type of regulation (A: Upregulation and B: Downregulation).

Mentions: Venn diagrams were used to compare the lists of 2-fold differentially expressed genes obtained for the three experimental groups of animals (Figure 2). Lists of upregulated and dowregulated genes were compared separately in order to find the same expression patterns between the dietary interventions compared (e.g. genes upregulated in both). Experimental groups A and B shared 84 upregulated and 31 downregulated genes, groups A and C displayed 80 upregulated and 21 downregulated genes in common and 276 upregulated and 117 downregulated genes were found in common for both groups B and C. A total of 89 genes were found to be differentially expressed in all three conditions due to CLA supplementation (76 up plus 13 down). These 89 genes are presented as Table 1. We also analyzed global changes in gene expression independently of the type of regulation. We observed that 34 genes displayed downregulation in group A (CLA transfer through milk) and upregulation in groups B/C (CLA transfer by oral gavage) (Data not shown). This behavior suggests a role for the way of CLA administration in modulating gene expression.


Gene expression profiles in rat mesenteric lymph nodes upon supplementation with conjugated linoleic acid during gestation and suckling.

Selga E, Pérez-Cano FJ, Franch A, Ramírez-Santana C, Rivero M, Ciudad CJ, Castellote C, Noé V - BMC Genomics (2011)

Venn Diagram of differentially expressed genes. Venn diagrams in GeneSpring GX were used to compare the lists of genes differentially expressed by 2-fold (p < 0.05) in each of the three experimental groups of animals. The diagrams show the number of genes that belong to each of the individual lists, the genes in common between each pair of lists and the genes in common among all three lists (in the center of the representation) for each type of regulation (A: Upregulation and B: Downregulation).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094308&req=5

Figure 2: Venn Diagram of differentially expressed genes. Venn diagrams in GeneSpring GX were used to compare the lists of genes differentially expressed by 2-fold (p < 0.05) in each of the three experimental groups of animals. The diagrams show the number of genes that belong to each of the individual lists, the genes in common between each pair of lists and the genes in common among all three lists (in the center of the representation) for each type of regulation (A: Upregulation and B: Downregulation).
Mentions: Venn diagrams were used to compare the lists of 2-fold differentially expressed genes obtained for the three experimental groups of animals (Figure 2). Lists of upregulated and dowregulated genes were compared separately in order to find the same expression patterns between the dietary interventions compared (e.g. genes upregulated in both). Experimental groups A and B shared 84 upregulated and 31 downregulated genes, groups A and C displayed 80 upregulated and 21 downregulated genes in common and 276 upregulated and 117 downregulated genes were found in common for both groups B and C. A total of 89 genes were found to be differentially expressed in all three conditions due to CLA supplementation (76 up plus 13 down). These 89 genes are presented as Table 1. We also analyzed global changes in gene expression independently of the type of regulation. We observed that 34 genes displayed downregulation in group A (CLA transfer through milk) and upregulation in groups B/C (CLA transfer by oral gavage) (Data not shown). This behavior suggests a role for the way of CLA administration in modulating gene expression.

Bottom Line: However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network.Gene underexpression was confirmed by Real-Time RT-PCR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Spain.

ABSTRACT

Background: Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.

Results: The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip(®) Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR.

Conclusions: Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.

Show MeSH
Related in: MedlinePlus