Limits...
Gene expression profiles in rat mesenteric lymph nodes upon supplementation with conjugated linoleic acid during gestation and suckling.

Selga E, Pérez-Cano FJ, Franch A, Ramírez-Santana C, Rivero M, Ciudad CJ, Castellote C, Noé V - BMC Genomics (2011)

Bottom Line: However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network.Gene underexpression was confirmed by Real-Time RT-PCR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Spain.

ABSTRACT

Background: Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.

Results: The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip(®) Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR.

Conclusions: Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.

Show MeSH

Related in: MedlinePlus

Experimental design. Total period of CLA supplementation (TPS) is shown in parentheses for each group of animals. Pups from dams fed with 1% CLA diet during the last two weeks of gestation and throughout the suckling period constituted Group A. Pups from dams fed only during the last two weeks of gestation with a 1% CLA diet and receiving CLA by daily oral gavage throughout the suckling period represented Group B. Pups from dams fed with a standard diet during the last two weeks of gestation and suckling and receiving CLA by daily oral gavage throughout the suckling period corresponded to Group C. Pups from dams fed with a standard diet throughout the study, constituted the reference diet group our Group D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3094308&req=5

Figure 1: Experimental design. Total period of CLA supplementation (TPS) is shown in parentheses for each group of animals. Pups from dams fed with 1% CLA diet during the last two weeks of gestation and throughout the suckling period constituted Group A. Pups from dams fed only during the last two weeks of gestation with a 1% CLA diet and receiving CLA by daily oral gavage throughout the suckling period represented Group B. Pups from dams fed with a standard diet during the last two weeks of gestation and suckling and receiving CLA by daily oral gavage throughout the suckling period corresponded to Group C. Pups from dams fed with a standard diet throughout the study, constituted the reference diet group our Group D.

Mentions: Wistar rats were subjected to a dietary supplementation during gestation and/or suckling with an 80:20 isomer mix of c9,t11 and t10,c12 CLA (Figure 1). CLA transfer was confirmed at day 21, when the proportion of CLA present in pup's plasma was around two times higher in groups A and B than that in group C, while values on reference group were very low [15]. At day 21, MLN were obtained and total RNA was prepared. The expression profile of MLN from control animals and supplemented with CLA was determined with the aid of the specific GeneChip® Rat Genome 230 2.0, which includes more than 28,000 rat genes. Data from these microarrays were uploaded in the database repository of Gene Expression Omnibus (GEO, [23]) and can be accessed through series accession number GSE23004. Upon normalization and statistical filtering using GeneSpring GX software package v10.0.2, lists of differentially expressed genes by 2-fold were built as described in Methods, and presented as additional files 1, 2 and 3. CLA supplementation during gestation and suckling (groups A and B) modulated the expression of 1332 genes whereas the dietary supplementation only during suckling modulated the expression of 517 genes. Moreover, CLA supplementation during gestation and suckling through dam's milk (group A) decreased the expression of 1001 genes and up-regulated 103 genes in MLN. The dietary supplementation during gestation and suckling by oral gavage (group B) decreased the expression of 257 genes and up-regulated 371 genes, whereas supplementation only during suckling (group C) decreased the expression of 271 genes and up-regulated 351 genes in MLN.


Gene expression profiles in rat mesenteric lymph nodes upon supplementation with conjugated linoleic acid during gestation and suckling.

Selga E, Pérez-Cano FJ, Franch A, Ramírez-Santana C, Rivero M, Ciudad CJ, Castellote C, Noé V - BMC Genomics (2011)

Experimental design. Total period of CLA supplementation (TPS) is shown in parentheses for each group of animals. Pups from dams fed with 1% CLA diet during the last two weeks of gestation and throughout the suckling period constituted Group A. Pups from dams fed only during the last two weeks of gestation with a 1% CLA diet and receiving CLA by daily oral gavage throughout the suckling period represented Group B. Pups from dams fed with a standard diet during the last two weeks of gestation and suckling and receiving CLA by daily oral gavage throughout the suckling period corresponded to Group C. Pups from dams fed with a standard diet throughout the study, constituted the reference diet group our Group D.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094308&req=5

Figure 1: Experimental design. Total period of CLA supplementation (TPS) is shown in parentheses for each group of animals. Pups from dams fed with 1% CLA diet during the last two weeks of gestation and throughout the suckling period constituted Group A. Pups from dams fed only during the last two weeks of gestation with a 1% CLA diet and receiving CLA by daily oral gavage throughout the suckling period represented Group B. Pups from dams fed with a standard diet during the last two weeks of gestation and suckling and receiving CLA by daily oral gavage throughout the suckling period corresponded to Group C. Pups from dams fed with a standard diet throughout the study, constituted the reference diet group our Group D.
Mentions: Wistar rats were subjected to a dietary supplementation during gestation and/or suckling with an 80:20 isomer mix of c9,t11 and t10,c12 CLA (Figure 1). CLA transfer was confirmed at day 21, when the proportion of CLA present in pup's plasma was around two times higher in groups A and B than that in group C, while values on reference group were very low [15]. At day 21, MLN were obtained and total RNA was prepared. The expression profile of MLN from control animals and supplemented with CLA was determined with the aid of the specific GeneChip® Rat Genome 230 2.0, which includes more than 28,000 rat genes. Data from these microarrays were uploaded in the database repository of Gene Expression Omnibus (GEO, [23]) and can be accessed through series accession number GSE23004. Upon normalization and statistical filtering using GeneSpring GX software package v10.0.2, lists of differentially expressed genes by 2-fold were built as described in Methods, and presented as additional files 1, 2 and 3. CLA supplementation during gestation and suckling (groups A and B) modulated the expression of 1332 genes whereas the dietary supplementation only during suckling modulated the expression of 517 genes. Moreover, CLA supplementation during gestation and suckling through dam's milk (group A) decreased the expression of 1001 genes and up-regulated 103 genes in MLN. The dietary supplementation during gestation and suckling by oral gavage (group B) decreased the expression of 257 genes and up-regulated 371 genes, whereas supplementation only during suckling (group C) decreased the expression of 271 genes and up-regulated 351 genes in MLN.

Bottom Line: However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network.Gene underexpression was confirmed by Real-Time RT-PCR.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, School of Pharmacy, University of Barcelona, Spain.

ABSTRACT

Background: Diet plays a role on the development of the immune system, and polyunsaturated fatty acids can modulate the expression of a variety of genes. Human milk contains conjugated linoleic acid (CLA), a fatty acid that seems to contribute to immune development. Indeed, recent studies carried out in our group in suckling animals have shown that the immune function is enhanced after feeding them with an 80:20 isomer mix composed of c9,t11 and t10,c12 CLA. However, little work has been done on the effects of CLA on gene expression, and even less regarding immune system development in early life.

Results: The expression profile of mesenteric lymph nodes from animals supplemented with CLA during gestation and suckling through dam's milk (Group A) or by oral gavage (Group B), supplemented just during suckling (Group C) and control animals (Group D) was determined with the aid of the specific GeneChip(®) Rat Genome 230 2.0 (Affymettrix). Bioinformatics analyses were performed using the GeneSpring GX software package v10.0.2 and lead to the identification of 89 genes differentially expressed in all three dietary approaches. Generation of a biological association network evidenced several genes, such as connective tissue growth factor (Ctgf), tissue inhibitor of metalloproteinase 1 (Timp1), galanin (Gal), synaptotagmin 1 (Syt1), growth factor receptor bound protein 2 (Grb2), actin gamma 2 (Actg2) and smooth muscle alpha actin (Acta2), as highly interconnected nodes of the resulting network. Gene underexpression was confirmed by Real-Time RT-PCR.

Conclusions: Ctgf, Timp1, Gal and Syt1, among others, are genes modulated by CLA supplementation that may have a role on mucosal immune responses in early life.

Show MeSH
Related in: MedlinePlus