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Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009.

Ji J, Xie J, Chen F, Shu D, Zuo K, Xue C, Qin J, Li H, Bi Y, Ma J, Xie Q - Virol. J. (2011)

Bottom Line: Phylogenetic analysis revealed that recent field isolates of IBV in China were mostly belonged to A2-branch (QXIBV-branch) and HN08-branch, only one isolate was belonged to Gray-branch and M41-branch respectively.Most of the 80 strains showed evolutionarily distant from vaccine strains.The results of this study suggested that nephropathogenic IBVs were mainly A2-like strains in China during 2008-2009.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT

Background: The nephropathogenic avian infectious bronchitis (IB) caused unprecedented economic losses to the commercial chicken industry of China in 2008-2009. To investigate the prevalence of nephropathogenic IB in China, eighty IBV isolates from different provinces during 2008-2009 were identified by dwarf embryo test and RT-PCR.

Results: The strains were mostly isolated in winter and spring with a wide age range of IB outbreaks, from 4 to 69 days. By the virus recovery trials, 70/80 of the strains resulted in the deaths or distresses of birds from nephritis. To learn more about the molecular evolutionary characteristics of the circulating field strains, the coding region of major spike 1 (S1) protein gene of these strains was RT-PCR amplified and sequenced. Compared to the published representative strains, nucleotides and amino acids sequence analysis indicated that the S1 genes of these strains and the reference strains displayed homologies ranging from 75.1% to 99.8% and from 73.1% to 99.8% respectively. S1 protein of the major pandemic strains contained 540 or 542 amino acids with the cleavage site of HRRRR or RRFRR. Phylogenetic analysis revealed that recent field isolates of IBV in China were mostly belonged to A2-branch (QXIBV-branch) and HN08-branch, only one isolate was belonged to Gray-branch and M41-branch respectively. Most of the 80 strains showed evolutionarily distant from vaccine strains.

Conclusions: The results of this study suggested that nephropathogenic IBVs were mainly A2-like strains in China during 2008-2009.

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Related in: MedlinePlus

The phylogenetic tree of IBVs isolated in mainland China during 2008-2009 based on the viral S1 sequences. The reference strains in this study were marked with "▲".
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Figure 1: The phylogenetic tree of IBVs isolated in mainland China during 2008-2009 based on the viral S1 sequences. The reference strains in this study were marked with "▲".

Mentions: A phylogenetic tree was constructed from the nucleotides sequences of the S1 glycoprotein genes. As shown in the Figure 1, the 80 isolates IBV strains were clustered into five distinct genetic groups or genotypes which were considerably heterogeneous, including A2-type (49 newly isolated strains), 4/91-type (9 newly isolated strains), HN08-type (20 newly isolated strains), Gray-type and M41-type. The newly isolated strains mainly belonged to A2-type, 4/91-type and HN08-type branch. The phylogenetic relationship of strains at different times and geographical regions displayed complexity and diversity.


Phylogenetic distribution and predominant genotype of the avian infectious bronchitis virus in China during 2008-2009.

Ji J, Xie J, Chen F, Shu D, Zuo K, Xue C, Qin J, Li H, Bi Y, Ma J, Xie Q - Virol. J. (2011)

The phylogenetic tree of IBVs isolated in mainland China during 2008-2009 based on the viral S1 sequences. The reference strains in this study were marked with "▲".
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094301&req=5

Figure 1: The phylogenetic tree of IBVs isolated in mainland China during 2008-2009 based on the viral S1 sequences. The reference strains in this study were marked with "▲".
Mentions: A phylogenetic tree was constructed from the nucleotides sequences of the S1 glycoprotein genes. As shown in the Figure 1, the 80 isolates IBV strains were clustered into five distinct genetic groups or genotypes which were considerably heterogeneous, including A2-type (49 newly isolated strains), 4/91-type (9 newly isolated strains), HN08-type (20 newly isolated strains), Gray-type and M41-type. The newly isolated strains mainly belonged to A2-type, 4/91-type and HN08-type branch. The phylogenetic relationship of strains at different times and geographical regions displayed complexity and diversity.

Bottom Line: Phylogenetic analysis revealed that recent field isolates of IBV in China were mostly belonged to A2-branch (QXIBV-branch) and HN08-branch, only one isolate was belonged to Gray-branch and M41-branch respectively.Most of the 80 strains showed evolutionarily distant from vaccine strains.The results of this study suggested that nephropathogenic IBVs were mainly A2-like strains in China during 2008-2009.

View Article: PubMed Central - HTML - PubMed

Affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT

Background: The nephropathogenic avian infectious bronchitis (IB) caused unprecedented economic losses to the commercial chicken industry of China in 2008-2009. To investigate the prevalence of nephropathogenic IB in China, eighty IBV isolates from different provinces during 2008-2009 were identified by dwarf embryo test and RT-PCR.

Results: The strains were mostly isolated in winter and spring with a wide age range of IB outbreaks, from 4 to 69 days. By the virus recovery trials, 70/80 of the strains resulted in the deaths or distresses of birds from nephritis. To learn more about the molecular evolutionary characteristics of the circulating field strains, the coding region of major spike 1 (S1) protein gene of these strains was RT-PCR amplified and sequenced. Compared to the published representative strains, nucleotides and amino acids sequence analysis indicated that the S1 genes of these strains and the reference strains displayed homologies ranging from 75.1% to 99.8% and from 73.1% to 99.8% respectively. S1 protein of the major pandemic strains contained 540 or 542 amino acids with the cleavage site of HRRRR or RRFRR. Phylogenetic analysis revealed that recent field isolates of IBV in China were mostly belonged to A2-branch (QXIBV-branch) and HN08-branch, only one isolate was belonged to Gray-branch and M41-branch respectively. Most of the 80 strains showed evolutionarily distant from vaccine strains.

Conclusions: The results of this study suggested that nephropathogenic IBVs were mainly A2-like strains in China during 2008-2009.

Show MeSH
Related in: MedlinePlus