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Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles.

Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Silva LF, Cavagna M, Baruffi RL, Oliveira JB, Franco JG - Reprod. Biol. Endocrinol. (2011)

Bottom Line: No relationship was observed between CM and ZP-BF positive/negative scores (P = 0.42).These variables had a low Pearson's correlation coefficient (r = 0.11).The current study demonstrated an absence of relationship between ZP-BF high/positive or low/negative score and nuclear and cytoplasmic in vitro maturation of oocytes from stimulation cycles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.

ABSTRACT

Background: This study evaluated whether there is a relationship between the zona pellucida birefringence (ZP-BF) intensity and the nuclear (NM) and cytoplasmic (CM) in vitro maturation of human oocytes from stimulated cycles.

Results: The ZP-BF was evaluated under an inverted microscope with a polarizing optical system and was scored as high/positive (when the ZP image presented a uniform and intense birefringence) or low/negative (when the image presented moderate and heterogeneous birefringence). CM was analyzed by evaluating the distribution of cortical granules (CGs) throughout the ooplasm by immunofluorescence staining. CM was classified as: complete, when CG was localized in the periphery; incomplete, when oocytes presented a cluster of CGs in the center; or in transition, when oocytes had both in clusters throughout cytoplasm and distributed in a layer in the cytoplasm periphery Nuclear maturation: From a total of 83 germinal vesicle (GV) stage oocytes, 58 of oocytes (69.9%) reached NM at the metaphase II stage. From these 58 oocytes matured in vitro, the high/positively scoring ZP-BF was presented in 82.7% of oocytes at the GV stage, in 75.8% of oocytes when at the metaphase I, and in 82.7% when oocytes reached MII. No relationship was observed between NM and ZP-BF positive/negative scores (P = 0.55). These variables had a low Pearson's correlation coefficient (r = 0.081). Cytoplasmic maturation: A total of 85 in vitro-matured MII oocytes were fixed for CM evaluation. Forty-nine oocytes of them (57.6%) showed the complete CM, 30 (61.2%) presented a high/positively scoring ZP-BF and 19 (38.8%) had a low/negatively scoring ZP-BF. From 36 oocytes (42.3%) with incomplete CM, 18 (50%) presented a high/positively scoring ZPBF and 18 (50%) had a low/negatively scoring ZP-BF. No relationship was observed between CM and ZP-BF positive/negative scores (P = 0.42). These variables had a low Pearson's correlation coefficient (r = 0.11).

Conclusions: The current study demonstrated an absence of relationship between ZP-BF high/positive or low/negative score and nuclear and cytoplasmic in vitro maturation of oocytes from stimulation cycles.

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Cytoplasmic Maturation. (A) Complete cytoplasmic maturation: CGs are located on the MII oocyte periphery. (B) Incomplete cytoplasmic maturation: CGs are both clustered in the center and on the periphery of the MII oocyte. CGs = cortical granules
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Figure 2: Cytoplasmic Maturation. (A) Complete cytoplasmic maturation: CGs are located on the MII oocyte periphery. (B) Incomplete cytoplasmic maturation: CGs are both clustered in the center and on the periphery of the MII oocyte. CGs = cortical granules

Mentions: CM was classified as complete (oocytes with all the CGs on the oocyte periphery; Figure 2A) or as incomplete (oocytes presenting CGs in cluster throughout cytoplasm or presenting both clusters and a layer in the oocyte periphery; Figure 2B).


Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles.

Petersen CG, Vagnini LD, Mauri AL, Massaro FC, Silva LF, Cavagna M, Baruffi RL, Oliveira JB, Franco JG - Reprod. Biol. Endocrinol. (2011)

Cytoplasmic Maturation. (A) Complete cytoplasmic maturation: CGs are located on the MII oocyte periphery. (B) Incomplete cytoplasmic maturation: CGs are both clustered in the center and on the periphery of the MII oocyte. CGs = cortical granules
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3094282&req=5

Figure 2: Cytoplasmic Maturation. (A) Complete cytoplasmic maturation: CGs are located on the MII oocyte periphery. (B) Incomplete cytoplasmic maturation: CGs are both clustered in the center and on the periphery of the MII oocyte. CGs = cortical granules
Mentions: CM was classified as complete (oocytes with all the CGs on the oocyte periphery; Figure 2A) or as incomplete (oocytes presenting CGs in cluster throughout cytoplasm or presenting both clusters and a layer in the oocyte periphery; Figure 2B).

Bottom Line: No relationship was observed between CM and ZP-BF positive/negative scores (P = 0.42).These variables had a low Pearson's correlation coefficient (r = 0.11).The current study demonstrated an absence of relationship between ZP-BF high/positive or low/negative score and nuclear and cytoplasmic in vitro maturation of oocytes from stimulation cycles.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Gynecology and Obstetrics, Botucatu Medical School, São Paulo State University-UNESP, Botucatu, Brazil.

ABSTRACT

Background: This study evaluated whether there is a relationship between the zona pellucida birefringence (ZP-BF) intensity and the nuclear (NM) and cytoplasmic (CM) in vitro maturation of human oocytes from stimulated cycles.

Results: The ZP-BF was evaluated under an inverted microscope with a polarizing optical system and was scored as high/positive (when the ZP image presented a uniform and intense birefringence) or low/negative (when the image presented moderate and heterogeneous birefringence). CM was analyzed by evaluating the distribution of cortical granules (CGs) throughout the ooplasm by immunofluorescence staining. CM was classified as: complete, when CG was localized in the periphery; incomplete, when oocytes presented a cluster of CGs in the center; or in transition, when oocytes had both in clusters throughout cytoplasm and distributed in a layer in the cytoplasm periphery Nuclear maturation: From a total of 83 germinal vesicle (GV) stage oocytes, 58 of oocytes (69.9%) reached NM at the metaphase II stage. From these 58 oocytes matured in vitro, the high/positively scoring ZP-BF was presented in 82.7% of oocytes at the GV stage, in 75.8% of oocytes when at the metaphase I, and in 82.7% when oocytes reached MII. No relationship was observed between NM and ZP-BF positive/negative scores (P = 0.55). These variables had a low Pearson's correlation coefficient (r = 0.081). Cytoplasmic maturation: A total of 85 in vitro-matured MII oocytes were fixed for CM evaluation. Forty-nine oocytes of them (57.6%) showed the complete CM, 30 (61.2%) presented a high/positively scoring ZP-BF and 19 (38.8%) had a low/negatively scoring ZP-BF. From 36 oocytes (42.3%) with incomplete CM, 18 (50%) presented a high/positively scoring ZPBF and 18 (50%) had a low/negatively scoring ZP-BF. No relationship was observed between CM and ZP-BF positive/negative scores (P = 0.42). These variables had a low Pearson's correlation coefficient (r = 0.11).

Conclusions: The current study demonstrated an absence of relationship between ZP-BF high/positive or low/negative score and nuclear and cytoplasmic in vitro maturation of oocytes from stimulation cycles.

Show MeSH
Related in: MedlinePlus