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Programming an in vitro DNA oscillator using a molecular networking strategy

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Measurement and simulation. (A) Schematic representation of the reactions included in the kinetic model. For clarity, the oligomers α, β and Inh have been omitted when not bound to a template. (B) Fluorescence signal (top panel, dashed line) and discrete concentration measurements of total individual oligomers α, β and Inh taken between t=235 and 520 min (Supplementary information S3). The plots show the mean of three replicates ±s.d. The solid curves are the best simultaneous agreement (‘optimized model') for fluorescence signal and oligomer concentrations, respectively (Supplementary information S5).
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f2: Measurement and simulation. (A) Schematic representation of the reactions included in the kinetic model. For clarity, the oligomers α, β and Inh have been omitted when not bound to a template. (B) Fluorescence signal (top panel, dashed line) and discrete concentration measurements of total individual oligomers α, β and Inh taken between t=235 and 520 min (Supplementary information S3). The plots show the mean of three replicates ±s.d. The solid curves are the best simultaneous agreement (‘optimized model') for fluorescence signal and oligomer concentrations, respectively (Supplementary information S5).

Mentions: ‘This created a slightly more complex, but also more realistic model, whose final parameters were optimized by fitting, simultaneously, all the data in Figure 2.'


Programming an in vitro DNA oscillator using a molecular networking strategy
Measurement and simulation. (A) Schematic representation of the reactions included in the kinetic model. For clarity, the oligomers α, β and Inh have been omitted when not bound to a template. (B) Fluorescence signal (top panel, dashed line) and discrete concentration measurements of total individual oligomers α, β and Inh taken between t=235 and 520 min (Supplementary information S3). The plots show the mean of three replicates ±s.d. The solid curves are the best simultaneous agreement (‘optimized model') for fluorescence signal and oligomer concentrations, respectively (Supplementary information S5).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3094062&req=5

f2: Measurement and simulation. (A) Schematic representation of the reactions included in the kinetic model. For clarity, the oligomers α, β and Inh have been omitted when not bound to a template. (B) Fluorescence signal (top panel, dashed line) and discrete concentration measurements of total individual oligomers α, β and Inh taken between t=235 and 520 min (Supplementary information S3). The plots show the mean of three replicates ±s.d. The solid curves are the best simultaneous agreement (‘optimized model') for fluorescence signal and oligomer concentrations, respectively (Supplementary information S5).
Mentions: ‘This created a slightly more complex, but also more realistic model, whose final parameters were optimized by fitting, simultaneously, all the data in Figure 2.'

View Article: PubMed Central

No MeSH data available.