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A seventeen-year-old female with hepatosplenic T-cell lymphoma associated with parvoviral infection.

Haque SA, Xiang Y, Ozdemirli M, Shad A, Kallakury B - Pediatr Rep (2010)

Bottom Line: We present an unusual case of a seventeen-year-old female, with no significant prior medical history, presenting with a hepatosplenic T-cell lymphoma.The diagnosis was confirmed by histological examination, immunohistochemisty, and flow cytometry.A staging work-up demonstrated bone marrow involvement by HSTL with concomitant intranuclear parvoviral inclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology;

ABSTRACT
Hepatosplenic T-cell lymphoma (HSTL) is rare, being derived from cytotoxic T-cells, and manifests as an extranodal systemic lymphoma. We present an unusual case of a seventeen-year-old female, with no significant prior medical history, presenting with a hepatosplenic T-cell lymphoma. The diagnosis was confirmed by histological examination, immunohistochemisty, and flow cytometry. A staging work-up demonstrated bone marrow involvement by HSTL with concomitant intranuclear parvoviral inclusions.

No MeSH data available.


Related in: MedlinePlus

Parvoviral nuclear inclusions in erythroblasts seen in a bone marrow biopsy (hematoxylin and eosin stain; 400× magnification). Inset: parvovirus immunostain.
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Figure 3: Parvoviral nuclear inclusions in erythroblasts seen in a bone marrow biopsy (hematoxylin and eosin stain; 400× magnification). Inset: parvovirus immunostain.

Mentions: Bilateral bone marrow aspirates and biopsies revealed a mildly hypocellular marrow with trilineage hemotopoiesis and involvement by HSTL. Flow cytometric analysis also demonstrated the above characteristic profile, notably CD5 deletion, supporting the diagnosis. Based on flow cytometry on the bone marrow, the neoplastic lymphocytes expressed T-cell receptor gamma/delta and lacked the expression of T-cell receptor alpha/beta. Large circumscribed intranuclear viral inclusions were present in the erythroid precursors (Figures 2 and 3), confirmed as a parvovirus infection by immunohistochemical staining (Figure 3, inset). The inclusions were eosinophilic, with a ground-glass appearance, and were compressing the chromatin against the nuclear membrane. PCR analysis was positive for monoclonal T-cell receptor gamma chain gene rearrangement and negative for EBNA. With the patient's history of massive hepatosplenomegaly, these morphologic and immunophenotypic findings were indicative of a hepatosplenic T-cell lymphoma, gamma/ delta type, associated with parvoviral infection.


A seventeen-year-old female with hepatosplenic T-cell lymphoma associated with parvoviral infection.

Haque SA, Xiang Y, Ozdemirli M, Shad A, Kallakury B - Pediatr Rep (2010)

Parvoviral nuclear inclusions in erythroblasts seen in a bone marrow biopsy (hematoxylin and eosin stain; 400× magnification). Inset: parvovirus immunostain.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3094005&req=5

Figure 3: Parvoviral nuclear inclusions in erythroblasts seen in a bone marrow biopsy (hematoxylin and eosin stain; 400× magnification). Inset: parvovirus immunostain.
Mentions: Bilateral bone marrow aspirates and biopsies revealed a mildly hypocellular marrow with trilineage hemotopoiesis and involvement by HSTL. Flow cytometric analysis also demonstrated the above characteristic profile, notably CD5 deletion, supporting the diagnosis. Based on flow cytometry on the bone marrow, the neoplastic lymphocytes expressed T-cell receptor gamma/delta and lacked the expression of T-cell receptor alpha/beta. Large circumscribed intranuclear viral inclusions were present in the erythroid precursors (Figures 2 and 3), confirmed as a parvovirus infection by immunohistochemical staining (Figure 3, inset). The inclusions were eosinophilic, with a ground-glass appearance, and were compressing the chromatin against the nuclear membrane. PCR analysis was positive for monoclonal T-cell receptor gamma chain gene rearrangement and negative for EBNA. With the patient's history of massive hepatosplenomegaly, these morphologic and immunophenotypic findings were indicative of a hepatosplenic T-cell lymphoma, gamma/ delta type, associated with parvoviral infection.

Bottom Line: We present an unusual case of a seventeen-year-old female, with no significant prior medical history, presenting with a hepatosplenic T-cell lymphoma.The diagnosis was confirmed by histological examination, immunohistochemisty, and flow cytometry.A staging work-up demonstrated bone marrow involvement by HSTL with concomitant intranuclear parvoviral inclusions.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology;

ABSTRACT
Hepatosplenic T-cell lymphoma (HSTL) is rare, being derived from cytotoxic T-cells, and manifests as an extranodal systemic lymphoma. We present an unusual case of a seventeen-year-old female, with no significant prior medical history, presenting with a hepatosplenic T-cell lymphoma. The diagnosis was confirmed by histological examination, immunohistochemisty, and flow cytometry. A staging work-up demonstrated bone marrow involvement by HSTL with concomitant intranuclear parvoviral inclusions.

No MeSH data available.


Related in: MedlinePlus