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Multiplex immunoassay of lower genital tract mucosal fluid from women attending an urban STD clinic shows broadly increased IL1ß and lactoferrin.

Spear GT, Kendrick SR, Chen HY, Thomas TT, Bahk M, Balderas R, Ghosh S, Weinberg A, Landay AL - PLoS ONE (2011)

Bottom Line: Samples from women with any STD/colonization had significantly higher levels of IL-8, IL-3, IL-7, IL-1ß, lactoferrin and myeloperoxidase.IL-1ß and lactoferrin were significantly increased in gonorrhea, Chlamydia, cervicitis, bacterial vaginosis and trichomoniasis.These studies show that mucosal fluid in general appears to be an environment that is rich in immune mediators.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology/Microbiology, Rush University Medical Center, Chicago, Illinois, United States of America. gspear@rush.edu

ABSTRACT

Background: More than one million new cases of sexually transmitted diseases (STDs) occur each day. The immune responses and inflammation induced by STDs and other frequent non-STD microbial colonizations (i.e. Candida and bacterial vaginosis) can have serious pathologic consequences in women including adverse pregnancy outcomes, infertility and increased susceptibility to infection by other pathogens. Understanding the types of immune mediators that are elicited in the lower genital tract by these infections/colonizations can give important insights into the innate and adaptive immune pathways that are activated and lead to strategies for preventing pathologic effects.

Methodology/principal findings: 32 immune mediators were measured by multiplexed immunoassays to assess the immune environment of the lower genital tract mucosa in 84 women attending an urban STD clinic. IL-3, IL-1ß, VEGF, angiogenin, IL-8, ß2Defensin and ß3Defensin were detected in all subjects, Interferon-α was detected in none, while the remaining mediators were detected in 40% to 93% of subjects. Angiogenin, VEGF, FGF, IL-9, IL-7, lymphotoxin-α and IL-3 had not been previously reported in genital mucosal fluid from women. Strong correlations were observed between levels of TNF-α, IL-1ß and IL-6, between chemokines IP-10 and MIG and between myeloperoxidase, IL-8 and G-CSF. Samples from women with any STD/colonization had significantly higher levels of IL-8, IL-3, IL-7, IL-1ß, lactoferrin and myeloperoxidase. IL-1ß and lactoferrin were significantly increased in gonorrhea, Chlamydia, cervicitis, bacterial vaginosis and trichomoniasis.

Conclusions/significance: These studies show that mucosal fluid in general appears to be an environment that is rich in immune mediators. Importantly, IL-1ß and lactoferrin are biomarkers for STDs/colonizations providing insights into immune responses and pathogenesis at this mucosal site.

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Heat map of cytokine levels.The heat map was generated using the web-based program Matrix2png [34]                            that displays microarray data visually (http://www.bioinformatics.ubc.ca/matrix2png/). The mean                            for each row of cytokine values is 0 with red representing values                            greater than 0, green lower than 0 and black 0.
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pone-0019560-g001: Heat map of cytokine levels.The heat map was generated using the web-based program Matrix2png [34] that displays microarray data visually (http://www.bioinformatics.ubc.ca/matrix2png/). The mean for each row of cytokine values is 0 with red representing values greater than 0, green lower than 0 and black 0.

Mentions: When viewed in a heat map format, differential patterns of increases in cytokines in relation to disease were apparent when comparing levels from subjects with trichomoniasis, bacterial vaginosis and controls (Fig. 1). Thus, IL-1ß, lactoferrin and IL-8 were increased in both trichomoniasis and BV when compared to controls. In contrast, IL-12, IL-7, IL-10, IL-3, and IL-4 were increased in BV when compared to control samples but not in trichomoniasis.


Multiplex immunoassay of lower genital tract mucosal fluid from women attending an urban STD clinic shows broadly increased IL1ß and lactoferrin.

Spear GT, Kendrick SR, Chen HY, Thomas TT, Bahk M, Balderas R, Ghosh S, Weinberg A, Landay AL - PLoS ONE (2011)

Heat map of cytokine levels.The heat map was generated using the web-based program Matrix2png [34]                            that displays microarray data visually (http://www.bioinformatics.ubc.ca/matrix2png/). The mean                            for each row of cytokine values is 0 with red representing values                            greater than 0, green lower than 0 and black 0.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3091877&req=5

pone-0019560-g001: Heat map of cytokine levels.The heat map was generated using the web-based program Matrix2png [34] that displays microarray data visually (http://www.bioinformatics.ubc.ca/matrix2png/). The mean for each row of cytokine values is 0 with red representing values greater than 0, green lower than 0 and black 0.
Mentions: When viewed in a heat map format, differential patterns of increases in cytokines in relation to disease were apparent when comparing levels from subjects with trichomoniasis, bacterial vaginosis and controls (Fig. 1). Thus, IL-1ß, lactoferrin and IL-8 were increased in both trichomoniasis and BV when compared to controls. In contrast, IL-12, IL-7, IL-10, IL-3, and IL-4 were increased in BV when compared to control samples but not in trichomoniasis.

Bottom Line: Samples from women with any STD/colonization had significantly higher levels of IL-8, IL-3, IL-7, IL-1ß, lactoferrin and myeloperoxidase.IL-1ß and lactoferrin were significantly increased in gonorrhea, Chlamydia, cervicitis, bacterial vaginosis and trichomoniasis.These studies show that mucosal fluid in general appears to be an environment that is rich in immune mediators.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology/Microbiology, Rush University Medical Center, Chicago, Illinois, United States of America. gspear@rush.edu

ABSTRACT

Background: More than one million new cases of sexually transmitted diseases (STDs) occur each day. The immune responses and inflammation induced by STDs and other frequent non-STD microbial colonizations (i.e. Candida and bacterial vaginosis) can have serious pathologic consequences in women including adverse pregnancy outcomes, infertility and increased susceptibility to infection by other pathogens. Understanding the types of immune mediators that are elicited in the lower genital tract by these infections/colonizations can give important insights into the innate and adaptive immune pathways that are activated and lead to strategies for preventing pathologic effects.

Methodology/principal findings: 32 immune mediators were measured by multiplexed immunoassays to assess the immune environment of the lower genital tract mucosa in 84 women attending an urban STD clinic. IL-3, IL-1ß, VEGF, angiogenin, IL-8, ß2Defensin and ß3Defensin were detected in all subjects, Interferon-α was detected in none, while the remaining mediators were detected in 40% to 93% of subjects. Angiogenin, VEGF, FGF, IL-9, IL-7, lymphotoxin-α and IL-3 had not been previously reported in genital mucosal fluid from women. Strong correlations were observed between levels of TNF-α, IL-1ß and IL-6, between chemokines IP-10 and MIG and between myeloperoxidase, IL-8 and G-CSF. Samples from women with any STD/colonization had significantly higher levels of IL-8, IL-3, IL-7, IL-1ß, lactoferrin and myeloperoxidase. IL-1ß and lactoferrin were significantly increased in gonorrhea, Chlamydia, cervicitis, bacterial vaginosis and trichomoniasis.

Conclusions/significance: These studies show that mucosal fluid in general appears to be an environment that is rich in immune mediators. Importantly, IL-1ß and lactoferrin are biomarkers for STDs/colonizations providing insights into immune responses and pathogenesis at this mucosal site.

Show MeSH
Related in: MedlinePlus