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Gene expression profile of the skin in the 'hairpoor' (HrHp) mice by microarray analysis.

Kim BK, Baek IC, Lee HY, Kim JK, Song HH, Yoon SK - BMC Genomics (2010)

Bottom Line: Among the 1861 genes with a > 1.2-fold increase in expression, further analysis showed that the expression of eight genes known to have a close relationship with hair follicle development, ascertained by conducting real-time PCR on skin RNA produced during hair follicle morphogenesis (P0-P14), indicated that four genes, Wif1, Casp14, Krt71, and Sfrp1, showed a consistent expression pattern with respect to HR overexpression in vivo.Wif1 and Casp14 were found to be upregulated, whereas Krt71 and Sfrp1 were downregulated in cells overexpressing HR in transient transfection experiments on keratinocytes, suggesting that HR may transcriptionally regulate these genes.Further studies are required to understand the mechanism of this regulation by the HR cofactor.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Sciences, The Catholic University of Korea, 505 Banpo-dong, Seoul 137-701, Korea.

ABSTRACT

Background: The transcriptional cofactor, Hairless (HR), acts as one of the key regulators of hair follicle cycling; the loss of function mutations is the cause of the expression of the hairless phenotype in humans and mice. Recently, we reported a new Hr mutant mouse called 'Hairpoor' (Hr(Hp)). These mutants harbor a gain of the function mutation, T403A, in the Hr gene. This confers the overexpression of HR and Hr(Hp) is an animal model of Marie Unna hereditary hypotrichosis in humans. In the present study, the expression profile of Hr(Hp)/Hr(Hp) skin was investigated using microarray analysis to identify genes whose expression was affected by the overexpression of HR.

Results: From 45,282 mouse probes, differential expressions in 43 (>2-fold), 306 (>1.5-fold), and 1861 genes (>1.2-fold) in skin from Hr(Hp)/Hr(Hp) mice were discovered and compared with skin from wild-type mice. Among the 1861 genes with a > 1.2-fold increase in expression, further analysis showed that the expression of eight genes known to have a close relationship with hair follicle development, ascertained by conducting real-time PCR on skin RNA produced during hair follicle morphogenesis (P0-P14), indicated that four genes, Wif1, Casp14, Krt71, and Sfrp1, showed a consistent expression pattern with respect to HR overexpression in vivo.

Conclusion: Wif1 and Casp14 were found to be upregulated, whereas Krt71 and Sfrp1 were downregulated in cells overexpressing HR in transient transfection experiments on keratinocytes, suggesting that HR may transcriptionally regulate these genes. Further studies are required to understand the mechanism of this regulation by the HR cofactor.

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Gene expression profile in HrHp/HrHp skin using microarray. (A) Hierarchical clustering represents differential expression of the genes between +/+ and HrHp/HrHp skins. (B) Using DEG finding criteria, we found differential expression in 43 (>2-fold) and 306 genes (>1.5-fold) in the skin from HrHp/HrHp mice compared with the skin from wild type mice.
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Figure 2: Gene expression profile in HrHp/HrHp skin using microarray. (A) Hierarchical clustering represents differential expression of the genes between +/+ and HrHp/HrHp skins. (B) Using DEG finding criteria, we found differential expression in 43 (>2-fold) and 306 genes (>1.5-fold) in the skin from HrHp/HrHp mice compared with the skin from wild type mice.

Mentions: Using 45,282 mouse probes, we performed microarray analysis and detected differential expression in 43 (>2-fold, p < 0.05), 306 (>1.5-fold, p < 0.05), and 1861 genes (>1.2-fold, p < 0.05) in the skin of HrHp/HrHp mice compared with the skin of +/+ mice at P0 (Figure 2, Table 1), listed in Table 2. Among the 43 genes with > 2-fold expression, 33 were downregulated in HrHp/HrHp skin at P0. The most strongly downregulated genes in the mutant skin included Cidea (0.14-fold), Cyp2g1 (0.18-fold) and Krt71 (0.3-fold). Contrasting this downregulation, the expression of 10 genes, Sepina3h (2.96-fold), Hmgcs2 (2.16-fold), and Odc1 (2.1-fold) in particular, were significantly upregulated despite HR being a transcriptional corepressor.


Gene expression profile of the skin in the 'hairpoor' (HrHp) mice by microarray analysis.

Kim BK, Baek IC, Lee HY, Kim JK, Song HH, Yoon SK - BMC Genomics (2010)

Gene expression profile in HrHp/HrHp skin using microarray. (A) Hierarchical clustering represents differential expression of the genes between +/+ and HrHp/HrHp skins. (B) Using DEG finding criteria, we found differential expression in 43 (>2-fold) and 306 genes (>1.5-fold) in the skin from HrHp/HrHp mice compared with the skin from wild type mice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3091768&req=5

Figure 2: Gene expression profile in HrHp/HrHp skin using microarray. (A) Hierarchical clustering represents differential expression of the genes between +/+ and HrHp/HrHp skins. (B) Using DEG finding criteria, we found differential expression in 43 (>2-fold) and 306 genes (>1.5-fold) in the skin from HrHp/HrHp mice compared with the skin from wild type mice.
Mentions: Using 45,282 mouse probes, we performed microarray analysis and detected differential expression in 43 (>2-fold, p < 0.05), 306 (>1.5-fold, p < 0.05), and 1861 genes (>1.2-fold, p < 0.05) in the skin of HrHp/HrHp mice compared with the skin of +/+ mice at P0 (Figure 2, Table 1), listed in Table 2. Among the 43 genes with > 2-fold expression, 33 were downregulated in HrHp/HrHp skin at P0. The most strongly downregulated genes in the mutant skin included Cidea (0.14-fold), Cyp2g1 (0.18-fold) and Krt71 (0.3-fold). Contrasting this downregulation, the expression of 10 genes, Sepina3h (2.96-fold), Hmgcs2 (2.16-fold), and Odc1 (2.1-fold) in particular, were significantly upregulated despite HR being a transcriptional corepressor.

Bottom Line: Among the 1861 genes with a > 1.2-fold increase in expression, further analysis showed that the expression of eight genes known to have a close relationship with hair follicle development, ascertained by conducting real-time PCR on skin RNA produced during hair follicle morphogenesis (P0-P14), indicated that four genes, Wif1, Casp14, Krt71, and Sfrp1, showed a consistent expression pattern with respect to HR overexpression in vivo.Wif1 and Casp14 were found to be upregulated, whereas Krt71 and Sfrp1 were downregulated in cells overexpressing HR in transient transfection experiments on keratinocytes, suggesting that HR may transcriptionally regulate these genes.Further studies are required to understand the mechanism of this regulation by the HR cofactor.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Biomedical Sciences, The Catholic University of Korea, 505 Banpo-dong, Seoul 137-701, Korea.

ABSTRACT

Background: The transcriptional cofactor, Hairless (HR), acts as one of the key regulators of hair follicle cycling; the loss of function mutations is the cause of the expression of the hairless phenotype in humans and mice. Recently, we reported a new Hr mutant mouse called 'Hairpoor' (Hr(Hp)). These mutants harbor a gain of the function mutation, T403A, in the Hr gene. This confers the overexpression of HR and Hr(Hp) is an animal model of Marie Unna hereditary hypotrichosis in humans. In the present study, the expression profile of Hr(Hp)/Hr(Hp) skin was investigated using microarray analysis to identify genes whose expression was affected by the overexpression of HR.

Results: From 45,282 mouse probes, differential expressions in 43 (>2-fold), 306 (>1.5-fold), and 1861 genes (>1.2-fold) in skin from Hr(Hp)/Hr(Hp) mice were discovered and compared with skin from wild-type mice. Among the 1861 genes with a > 1.2-fold increase in expression, further analysis showed that the expression of eight genes known to have a close relationship with hair follicle development, ascertained by conducting real-time PCR on skin RNA produced during hair follicle morphogenesis (P0-P14), indicated that four genes, Wif1, Casp14, Krt71, and Sfrp1, showed a consistent expression pattern with respect to HR overexpression in vivo.

Conclusion: Wif1 and Casp14 were found to be upregulated, whereas Krt71 and Sfrp1 were downregulated in cells overexpressing HR in transient transfection experiments on keratinocytes, suggesting that HR may transcriptionally regulate these genes. Further studies are required to understand the mechanism of this regulation by the HR cofactor.

Show MeSH
Related in: MedlinePlus