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The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.

Zhang JY, Pan MH, Sun ZY, Huang SJ, Yu ZS, Liu D, Zhao DH, Lu C - BMC Genomics (2010)

Bottom Line: However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system.Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.These results lay the foundation for further apoptosis-related study in Bombyx mori.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Sericultural Laboratory of Agricultural Ministry, Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400715, China.

ABSTRACT

Background: Apoptosis is regulated in an orderly fashion by a series of genes, and has a crucial role in important physiological processes such as growth development, immunological response and so on. Recently, substantial studies have been undertaken on apoptosis in model animals including humans, fruit flies, and the nematode. However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system. Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.

Results: From the newly assembled genome databases, a genome-wide analysis of apoptosis-related genes in Bombyx mori was performed using both nucleotide and protein Blast searches. Fifty-two apoptosis-related candidate genes were identified, including five caspase family members, two tumor necrosis factor (TNF) superfamily members, one Bcl-2 family member, four baculovirus IAP (inhibitor of apoptosis) repeat (BIR) domain family members and 1 RHG (Reaper, Hid, Grim, and Sickle; Drosophila cell death activators) family member. Moreover, we identified a new caspase family member, BmCaspase-New, two splice variants of BmDronc, and Bm3585, a mammalian TNF superfamily member homolog. Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells. Sequence analyses revealed that these genes could have key roles in apoptosis.

Conclusions: Bombyx mori possesses potential apoptosis-related genes. We hypothesized that the classic intrinsic and extrinsic apoptotic pathways potentially are active in Bombyx mori. These results lay the foundation for further apoptosis-related study in Bombyx mori.

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Related in: MedlinePlus

RT-PCR analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages and in BmE cells. Total RNA was isolated as described in the Methods section and analyzed by RT-PCR. W48: about 48 h after wandering; W96: about 96 h after wandering; W9D: about 9th day after wandering; M: silk moth; B_N: normal BmE cells; B_A: BmE cells exposed to actinomycin D; B_U: BmE cells exposed to UV irradiation.
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Figure 7: RT-PCR analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages and in BmE cells. Total RNA was isolated as described in the Methods section and analyzed by RT-PCR. W48: about 48 h after wandering; W96: about 96 h after wandering; W9D: about 9th day after wandering; M: silk moth; B_N: normal BmE cells; B_A: BmE cells exposed to actinomycin D; B_U: BmE cells exposed to UV irradiation.

Mentions: To test the expression of the apoptosis genes, we designed primers for 32 genes according to their predicted DNA sequences (Additional file 2) and carried out RT-PCR using the cDNA isolated from the different development stages of silkworm and BmE cells exposed to different stressors as described in the Methods section. Twenty three of all apoptosis-related genes tested were cloned and sequenced, five apoptosis-related genes were detected by RT-PCR but not sequenced successfully, although the PCR product sizes were consistent with the predicted sizes, and four silkworm apoptosis-related genes were not detected (Additional file 2). Overall, the expression of these genes, except a few, was relatively much lower than BmActin3 expression (Figure 7). The results show that most of the key apoptosis-genes in silkworm are expressed, which revealed that these potential apoptosis-genes are present in Bombyx mori.


The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.

Zhang JY, Pan MH, Sun ZY, Huang SJ, Yu ZS, Liu D, Zhao DH, Lu C - BMC Genomics (2010)

RT-PCR analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages and in BmE cells. Total RNA was isolated as described in the Methods section and analyzed by RT-PCR. W48: about 48 h after wandering; W96: about 96 h after wandering; W9D: about 9th day after wandering; M: silk moth; B_N: normal BmE cells; B_A: BmE cells exposed to actinomycin D; B_U: BmE cells exposed to UV irradiation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3091752&req=5

Figure 7: RT-PCR analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages and in BmE cells. Total RNA was isolated as described in the Methods section and analyzed by RT-PCR. W48: about 48 h after wandering; W96: about 96 h after wandering; W9D: about 9th day after wandering; M: silk moth; B_N: normal BmE cells; B_A: BmE cells exposed to actinomycin D; B_U: BmE cells exposed to UV irradiation.
Mentions: To test the expression of the apoptosis genes, we designed primers for 32 genes according to their predicted DNA sequences (Additional file 2) and carried out RT-PCR using the cDNA isolated from the different development stages of silkworm and BmE cells exposed to different stressors as described in the Methods section. Twenty three of all apoptosis-related genes tested were cloned and sequenced, five apoptosis-related genes were detected by RT-PCR but not sequenced successfully, although the PCR product sizes were consistent with the predicted sizes, and four silkworm apoptosis-related genes were not detected (Additional file 2). Overall, the expression of these genes, except a few, was relatively much lower than BmActin3 expression (Figure 7). The results show that most of the key apoptosis-genes in silkworm are expressed, which revealed that these potential apoptosis-genes are present in Bombyx mori.

Bottom Line: However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system.Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.These results lay the foundation for further apoptosis-related study in Bombyx mori.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Sericultural Laboratory of Agricultural Ministry, Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400715, China.

ABSTRACT

Background: Apoptosis is regulated in an orderly fashion by a series of genes, and has a crucial role in important physiological processes such as growth development, immunological response and so on. Recently, substantial studies have been undertaken on apoptosis in model animals including humans, fruit flies, and the nematode. However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system. Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.

Results: From the newly assembled genome databases, a genome-wide analysis of apoptosis-related genes in Bombyx mori was performed using both nucleotide and protein Blast searches. Fifty-two apoptosis-related candidate genes were identified, including five caspase family members, two tumor necrosis factor (TNF) superfamily members, one Bcl-2 family member, four baculovirus IAP (inhibitor of apoptosis) repeat (BIR) domain family members and 1 RHG (Reaper, Hid, Grim, and Sickle; Drosophila cell death activators) family member. Moreover, we identified a new caspase family member, BmCaspase-New, two splice variants of BmDronc, and Bm3585, a mammalian TNF superfamily member homolog. Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells. Sequence analyses revealed that these genes could have key roles in apoptosis.

Conclusions: Bombyx mori possesses potential apoptosis-related genes. We hypothesized that the classic intrinsic and extrinsic apoptotic pathways potentially are active in Bombyx mori. These results lay the foundation for further apoptosis-related study in Bombyx mori.

Show MeSH
Related in: MedlinePlus