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The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.

Zhang JY, Pan MH, Sun ZY, Huang SJ, Yu ZS, Liu D, Zhao DH, Lu C - BMC Genomics (2010)

Bottom Line: However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system.Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.These results lay the foundation for further apoptosis-related study in Bombyx mori.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Sericultural Laboratory of Agricultural Ministry, Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400715, China.

ABSTRACT

Background: Apoptosis is regulated in an orderly fashion by a series of genes, and has a crucial role in important physiological processes such as growth development, immunological response and so on. Recently, substantial studies have been undertaken on apoptosis in model animals including humans, fruit flies, and the nematode. However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system. Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.

Results: From the newly assembled genome databases, a genome-wide analysis of apoptosis-related genes in Bombyx mori was performed using both nucleotide and protein Blast searches. Fifty-two apoptosis-related candidate genes were identified, including five caspase family members, two tumor necrosis factor (TNF) superfamily members, one Bcl-2 family member, four baculovirus IAP (inhibitor of apoptosis) repeat (BIR) domain family members and 1 RHG (Reaper, Hid, Grim, and Sickle; Drosophila cell death activators) family member. Moreover, we identified a new caspase family member, BmCaspase-New, two splice variants of BmDronc, and Bm3585, a mammalian TNF superfamily member homolog. Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells. Sequence analyses revealed that these genes could have key roles in apoptosis.

Conclusions: Bombyx mori possesses potential apoptosis-related genes. We hypothesized that the classic intrinsic and extrinsic apoptotic pathways potentially are active in Bombyx mori. These results lay the foundation for further apoptosis-related study in Bombyx mori.

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Related in: MedlinePlus

Microarray analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages. The expression levels of all the genes in different stages compare to their expression level in the 3th day of fifth instar. V3: 3th of fifth instar; W0: 0 h after wandering; W12: 12 h after wandering; W24: 24 h after wandering; W36: 36 h after wandering; W48: 48 h after wandering; W60: 60 h after wandering; W72: 72 h after wandering; W96: 96 h after wandering; W120: 120 h after wandering; W6D: 6th day after wandering; W7D: 7th day after wandering; W8D: 8th day after wandering; W9D: 9th after wandering; M: silk moth. Red is a ratio ≥1, green is a ratio ≤1, and black is a ratio = 1.
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Figure 6: Microarray analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages. The expression levels of all the genes in different stages compare to their expression level in the 3th day of fifth instar. V3: 3th of fifth instar; W0: 0 h after wandering; W12: 12 h after wandering; W24: 24 h after wandering; W36: 36 h after wandering; W48: 48 h after wandering; W60: 60 h after wandering; W72: 72 h after wandering; W96: 96 h after wandering; W120: 120 h after wandering; W6D: 6th day after wandering; W7D: 7th day after wandering; W8D: 8th day after wandering; W9D: 9th after wandering; M: silk moth. Red is a ratio ≥1, green is a ratio ≤1, and black is a ratio = 1.

Mentions: To analyze the expression of the silkworm apoptosis-related genes in different developmental stages according the chips, a BlastN alignment was performed using the silkworm different developmental stage database. The results indicated that all the apoptosis-related genes contained at least one oligonucleotide probes except BmDredd, BmFadd, BmGsk3 (Table 1), for which no probe was found. Only 26 apoptosis-genes show higher expression than in the 3rd day of the fifth instar, when almost all genes expressed in the silkworm are present (Figure 6). The results revealed that the expressions of apoptosis-genes are relative low in silkworm.


The genomic underpinnings of apoptosis in the silkworm, Bombyx mori.

Zhang JY, Pan MH, Sun ZY, Huang SJ, Yu ZS, Liu D, Zhao DH, Lu C - BMC Genomics (2010)

Microarray analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages. The expression levels of all the genes in different stages compare to their expression level in the 3th day of fifth instar. V3: 3th of fifth instar; W0: 0 h after wandering; W12: 12 h after wandering; W24: 24 h after wandering; W36: 36 h after wandering; W48: 48 h after wandering; W60: 60 h after wandering; W72: 72 h after wandering; W96: 96 h after wandering; W120: 120 h after wandering; W6D: 6th day after wandering; W7D: 7th day after wandering; W8D: 8th day after wandering; W9D: 9th after wandering; M: silk moth. Red is a ratio ≥1, green is a ratio ≤1, and black is a ratio = 1.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3091752&req=5

Figure 6: Microarray analysis of the expression of the putative silkworm apoptosis-related genes during different growth stages. The expression levels of all the genes in different stages compare to their expression level in the 3th day of fifth instar. V3: 3th of fifth instar; W0: 0 h after wandering; W12: 12 h after wandering; W24: 24 h after wandering; W36: 36 h after wandering; W48: 48 h after wandering; W60: 60 h after wandering; W72: 72 h after wandering; W96: 96 h after wandering; W120: 120 h after wandering; W6D: 6th day after wandering; W7D: 7th day after wandering; W8D: 8th day after wandering; W9D: 9th after wandering; M: silk moth. Red is a ratio ≥1, green is a ratio ≤1, and black is a ratio = 1.
Mentions: To analyze the expression of the silkworm apoptosis-related genes in different developmental stages according the chips, a BlastN alignment was performed using the silkworm different developmental stage database. The results indicated that all the apoptosis-related genes contained at least one oligonucleotide probes except BmDredd, BmFadd, BmGsk3 (Table 1), for which no probe was found. Only 26 apoptosis-genes show higher expression than in the 3rd day of the fifth instar, when almost all genes expressed in the silkworm are present (Figure 6). The results revealed that the expressions of apoptosis-genes are relative low in silkworm.

Bottom Line: However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system.Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.These results lay the foundation for further apoptosis-related study in Bombyx mori.

View Article: PubMed Central - HTML - PubMed

Affiliation: The Key Sericultural Laboratory of Agricultural Ministry, Institute of Sericulture and Systems Biology, Southwest University, Chongqing 400715, China.

ABSTRACT

Background: Apoptosis is regulated in an orderly fashion by a series of genes, and has a crucial role in important physiological processes such as growth development, immunological response and so on. Recently, substantial studies have been undertaken on apoptosis in model animals including humans, fruit flies, and the nematode. However, the lack of genomic data for silkworms limits their usefulness in apoptosis studies, despite the advantages of silkworm as a representative of Lepidoptera and an effective model system. Herein we have identified apoptosis-related genes in the silkworm Bombyx mori and compared them to those from insects, mammals, and nematodes.

Results: From the newly assembled genome databases, a genome-wide analysis of apoptosis-related genes in Bombyx mori was performed using both nucleotide and protein Blast searches. Fifty-two apoptosis-related candidate genes were identified, including five caspase family members, two tumor necrosis factor (TNF) superfamily members, one Bcl-2 family member, four baculovirus IAP (inhibitor of apoptosis) repeat (BIR) domain family members and 1 RHG (Reaper, Hid, Grim, and Sickle; Drosophila cell death activators) family member. Moreover, we identified a new caspase family member, BmCaspase-New, two splice variants of BmDronc, and Bm3585, a mammalian TNF superfamily member homolog. Twenty-three of these apoptosis-related genes were cloned and sequenced using cDNA templates isolated from BmE-SWU1 cells. Sequence analyses revealed that these genes could have key roles in apoptosis.

Conclusions: Bombyx mori possesses potential apoptosis-related genes. We hypothesized that the classic intrinsic and extrinsic apoptotic pathways potentially are active in Bombyx mori. These results lay the foundation for further apoptosis-related study in Bombyx mori.

Show MeSH
Related in: MedlinePlus