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Differential gene expression analysis of placentas with increased vascular resistance and pre-eclampsia using whole-genome microarrays.

Centlow M, Wingren C, Borrebaeck C, Brownstein MJ, Hansson SR - J Pregnancy (2011)

Bottom Line: In order to search for genes associated with protection or progression, whole-genome profiling was performed.The microarray showed 148 genes to be significantly altered between the four groups.In the preeclamptic group compared to notch only, there was increased expression of genes related to chemotaxis and the NF-kappa B pathway and decreased expression of genes related to antigen processing and presentation, such as human leukocyte antigen B.

View Article: PubMed Central - PubMed

Affiliation: Departments of Obstetrics and Gynecology and Clinical Sciences, Lund University, BMC C14, Klinikgatan 28, 221 84 Lund, Sweden.

ABSTRACT
Pre-eclampsia is a pregnancy complication characterized by hypertension and proteinuria. There are several factors associated with an increased risk of developing pre-eclampsia, one of which is increased uterine artery resistance, referred to as "notching". However, some women do not progress into pre-eclampsia whereas others may have a higher risk of doing so. The placenta, central in pre-eclampsia pathology, may express genes associated with either protection or progression into pre-eclampsia. In order to search for genes associated with protection or progression, whole-genome profiling was performed. Placental tissue from 15 controls, 10 pre-eclamptic, 5 pre-eclampsia with notching, and 5 with notching only were analyzed using microarray and antibody microarrays to study some of the same gene product and functionally related ones. The microarray showed 148 genes to be significantly altered between the four groups. In the preeclamptic group compared to notch only, there was increased expression of genes related to chemotaxis and the NF-kappa B pathway and decreased expression of genes related to antigen processing and presentation, such as human leukocyte antigen B. Our results indicate that progression of pre-eclampsia from notching may involve the development of inflammation. Increased expression of antigen-presenting genes, as seen in the notch-only placenta, may prevent this inflammatory response and, thereby, protect the patient from developing pre-eclampsia.

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Related in: MedlinePlus

Microarray validation with real-time PCR.  The most significantly altered genes in the microarray analysis as well as genes of interest were validated using quantitative real-time PCR. Results are presented as box-plots, showing groups' medians as well as the 25th and the 75th percentile. β-actin was used as housekeeping gene, and all values are quotas between the gene of interest and β-actin. Kruskal-Wallis with post hoc Dunn was used to determine statistical significance. *P < .05, **P < .01, ***P < .005  The order is as follows: (a) inhibin α, (b) transforming growth factor β, (c) Tat-interacting protein (30 kD), and (d) haptoglobin.
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fig1: Microarray validation with real-time PCR. The most significantly altered genes in the microarray analysis as well as genes of interest were validated using quantitative real-time PCR. Results are presented as box-plots, showing groups' medians as well as the 25th and the 75th percentile. β-actin was used as housekeeping gene, and all values are quotas between the gene of interest and β-actin. Kruskal-Wallis with post hoc Dunn was used to determine statistical significance. *P < .05, **P < .01, ***P < .005 The order is as follows: (a) inhibin α, (b) transforming growth factor β, (c) Tat-interacting protein (30 kD), and (d) haptoglobin.

Mentions: Quantitative real-time PCR was used to verify the gene microarray results. Real-time mRNA data are presented as box-plots in Figure 1.


Differential gene expression analysis of placentas with increased vascular resistance and pre-eclampsia using whole-genome microarrays.

Centlow M, Wingren C, Borrebaeck C, Brownstein MJ, Hansson SR - J Pregnancy (2011)

Microarray validation with real-time PCR.  The most significantly altered genes in the microarray analysis as well as genes of interest were validated using quantitative real-time PCR. Results are presented as box-plots, showing groups' medians as well as the 25th and the 75th percentile. β-actin was used as housekeeping gene, and all values are quotas between the gene of interest and β-actin. Kruskal-Wallis with post hoc Dunn was used to determine statistical significance. *P < .05, **P < .01, ***P < .005  The order is as follows: (a) inhibin α, (b) transforming growth factor β, (c) Tat-interacting protein (30 kD), and (d) haptoglobin.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3066560&req=5

fig1: Microarray validation with real-time PCR. The most significantly altered genes in the microarray analysis as well as genes of interest were validated using quantitative real-time PCR. Results are presented as box-plots, showing groups' medians as well as the 25th and the 75th percentile. β-actin was used as housekeeping gene, and all values are quotas between the gene of interest and β-actin. Kruskal-Wallis with post hoc Dunn was used to determine statistical significance. *P < .05, **P < .01, ***P < .005 The order is as follows: (a) inhibin α, (b) transforming growth factor β, (c) Tat-interacting protein (30 kD), and (d) haptoglobin.
Mentions: Quantitative real-time PCR was used to verify the gene microarray results. Real-time mRNA data are presented as box-plots in Figure 1.

Bottom Line: In order to search for genes associated with protection or progression, whole-genome profiling was performed.The microarray showed 148 genes to be significantly altered between the four groups.In the preeclamptic group compared to notch only, there was increased expression of genes related to chemotaxis and the NF-kappa B pathway and decreased expression of genes related to antigen processing and presentation, such as human leukocyte antigen B.

View Article: PubMed Central - PubMed

Affiliation: Departments of Obstetrics and Gynecology and Clinical Sciences, Lund University, BMC C14, Klinikgatan 28, 221 84 Lund, Sweden.

ABSTRACT
Pre-eclampsia is a pregnancy complication characterized by hypertension and proteinuria. There are several factors associated with an increased risk of developing pre-eclampsia, one of which is increased uterine artery resistance, referred to as "notching". However, some women do not progress into pre-eclampsia whereas others may have a higher risk of doing so. The placenta, central in pre-eclampsia pathology, may express genes associated with either protection or progression into pre-eclampsia. In order to search for genes associated with protection or progression, whole-genome profiling was performed. Placental tissue from 15 controls, 10 pre-eclamptic, 5 pre-eclampsia with notching, and 5 with notching only were analyzed using microarray and antibody microarrays to study some of the same gene product and functionally related ones. The microarray showed 148 genes to be significantly altered between the four groups. In the preeclamptic group compared to notch only, there was increased expression of genes related to chemotaxis and the NF-kappa B pathway and decreased expression of genes related to antigen processing and presentation, such as human leukocyte antigen B. Our results indicate that progression of pre-eclampsia from notching may involve the development of inflammation. Increased expression of antigen-presenting genes, as seen in the notch-only placenta, may prevent this inflammatory response and, thereby, protect the patient from developing pre-eclampsia.

Show MeSH
Related in: MedlinePlus