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Procyanidins are potent inhibitors of LOX-1: a new player in the French Paradox.

Nishizuka T, Fujita Y, Sato Y, Nakano A, Kakino A, Ohshima S, Kanda T, Yoshimoto R, Sawamura T - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2011)

Bottom Line: Indeed, purified procyanidins significantly inhibited oxLDL binding to LOX-1 while other ingredients of apple polyphenols did not.Moreover, chronic administration of oligomeric procyanidins suppressed lipid accumulation in vascular wall in hypertensive rats fed with high fat diet.These results suggest that procyanidins are LOX-1 inhibitors and LOX-1 inhibition might be a possible underlying mechanism of the well-known vascular protective effects of red wine, the French Paradox.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Physiology, National Cerebral and Cardiovascular Center, Osaka, Japan.

ABSTRACT
Lectin-like oxidized LDL receptor-1 (LOX-1) is an endothelial receptor for oxidized LDL (oxLDL) and plays multiple roles in the development of cardiovascular diseases. We screened more than 400 foodstuff extracts for identifying materials that inhibit oxLDL binding to LOX-1. Results showed that 52 extracts inhibited LOX-1 by more than 70% in cell-free assays. Subsequent cell-based assays revealed that a variety of foodstuffs known to be rich in procyanidins such as grape seed extracts and apple polyphenols, potently inhibited oxLDL uptake in Chinese hamster ovary (CHO) cells expressing LOX-1. Indeed, purified procyanidins significantly inhibited oxLDL binding to LOX-1 while other ingredients of apple polyphenols did not. Moreover, chronic administration of oligomeric procyanidins suppressed lipid accumulation in vascular wall in hypertensive rats fed with high fat diet. These results suggest that procyanidins are LOX-1 inhibitors and LOX-1 inhibition might be a possible underlying mechanism of the well-known vascular protective effects of red wine, the French Paradox.

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Related in: MedlinePlus

Arterial lipid deposition in SHR-SP rats on high fat diet. Oil Red-O image (A) and the number of lipid deposition (B) in control and OPC rats. * represents statistical significance from control.
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fig05: Arterial lipid deposition in SHR-SP rats on high fat diet. Oil Red-O image (A) and the number of lipid deposition (B) in control and OPC rats. * represents statistical significance from control.

Mentions: Finally, we addressed whether procyanidins inhibited lipid accumulation in vascular wall in vivo. Oligomeric procyanidins (OPC), a mixture containing procyanidins with various chain lengths purified from apple, was used for in vivo study. SHR-SP rats are well-known disease-model animals for hypertension and stroke, and accumulate lipids in arterial wall in response to high fat diet.22,24) In this study, 8 week old SHR-SP rats were given high fat diet and physiological saline containing 0.5% OPC (OPC rats) for 2 weeks. OPC did not affect the daily food intake and body weight during the experimental period. Systolic and diastolic blood pressure and heart rates were also unaffected (Table 2). At termination, the mesenteric artery isolated from the control rats displayed numerous Oil Red O-positive dots while that from the OPC-treated rats displayed significantly less dots, indicating that OPC suppressed arterial lipid deposition (Fig. 5). Plasma total cholesterol (TC), HDL-cholesterol, triglyceride and TBARS did not differ between the groups (Table 2). The plasma concentrations of procyanidin B2 and C1 in OPC rats were 7.9 ± 1.8 ng/mL and 4.9 ± 1.9 ng/mL, respectively.


Procyanidins are potent inhibitors of LOX-1: a new player in the French Paradox.

Nishizuka T, Fujita Y, Sato Y, Nakano A, Kakino A, Ohshima S, Kanda T, Yoshimoto R, Sawamura T - Proc. Jpn. Acad., Ser. B, Phys. Biol. Sci. (2011)

Arterial lipid deposition in SHR-SP rats on high fat diet. Oil Red-O image (A) and the number of lipid deposition (B) in control and OPC rats. * represents statistical significance from control.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3066543&req=5

fig05: Arterial lipid deposition in SHR-SP rats on high fat diet. Oil Red-O image (A) and the number of lipid deposition (B) in control and OPC rats. * represents statistical significance from control.
Mentions: Finally, we addressed whether procyanidins inhibited lipid accumulation in vascular wall in vivo. Oligomeric procyanidins (OPC), a mixture containing procyanidins with various chain lengths purified from apple, was used for in vivo study. SHR-SP rats are well-known disease-model animals for hypertension and stroke, and accumulate lipids in arterial wall in response to high fat diet.22,24) In this study, 8 week old SHR-SP rats were given high fat diet and physiological saline containing 0.5% OPC (OPC rats) for 2 weeks. OPC did not affect the daily food intake and body weight during the experimental period. Systolic and diastolic blood pressure and heart rates were also unaffected (Table 2). At termination, the mesenteric artery isolated from the control rats displayed numerous Oil Red O-positive dots while that from the OPC-treated rats displayed significantly less dots, indicating that OPC suppressed arterial lipid deposition (Fig. 5). Plasma total cholesterol (TC), HDL-cholesterol, triglyceride and TBARS did not differ between the groups (Table 2). The plasma concentrations of procyanidin B2 and C1 in OPC rats were 7.9 ± 1.8 ng/mL and 4.9 ± 1.9 ng/mL, respectively.

Bottom Line: Indeed, purified procyanidins significantly inhibited oxLDL binding to LOX-1 while other ingredients of apple polyphenols did not.Moreover, chronic administration of oligomeric procyanidins suppressed lipid accumulation in vascular wall in hypertensive rats fed with high fat diet.These results suggest that procyanidins are LOX-1 inhibitors and LOX-1 inhibition might be a possible underlying mechanism of the well-known vascular protective effects of red wine, the French Paradox.

View Article: PubMed Central - PubMed

Affiliation: Department of Vascular Physiology, National Cerebral and Cardiovascular Center, Osaka, Japan.

ABSTRACT
Lectin-like oxidized LDL receptor-1 (LOX-1) is an endothelial receptor for oxidized LDL (oxLDL) and plays multiple roles in the development of cardiovascular diseases. We screened more than 400 foodstuff extracts for identifying materials that inhibit oxLDL binding to LOX-1. Results showed that 52 extracts inhibited LOX-1 by more than 70% in cell-free assays. Subsequent cell-based assays revealed that a variety of foodstuffs known to be rich in procyanidins such as grape seed extracts and apple polyphenols, potently inhibited oxLDL uptake in Chinese hamster ovary (CHO) cells expressing LOX-1. Indeed, purified procyanidins significantly inhibited oxLDL binding to LOX-1 while other ingredients of apple polyphenols did not. Moreover, chronic administration of oligomeric procyanidins suppressed lipid accumulation in vascular wall in hypertensive rats fed with high fat diet. These results suggest that procyanidins are LOX-1 inhibitors and LOX-1 inhibition might be a possible underlying mechanism of the well-known vascular protective effects of red wine, the French Paradox.

Show MeSH
Related in: MedlinePlus