Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
Bottom Line: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray.We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction.Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.
Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.Show MeSH
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Mentions: LAIR-2 has been shown to be a soluble antagonist of the closely related LAIR-1 receptor that is expressed by all peripheral leukocytes [26,27]. Using flow cytometry of first trimester decidual leukocytes, we found that LAIR-1 is expressed by all CD3+ and HLA-DR+ cells, and ∼50% of the CD56 + cell population (Fig. 6A). Immuno-histological staining of the decidua basalis showed that these LAIR-1+ leukocytes are present underlying glandular epithelium and around blood vessels as well as scattered throughout the decidua (Fig. 6B).
Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.