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Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.

Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A - Placenta (2010)

Bottom Line: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray.We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction.Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

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Related in: MedlinePlus

LAIR-1 expression in the decidua. Decidual leukocytes isolated from first trimester pregnancies were analysed by surface flow cytometry (A). The scatter gate R1 was used for CD45, CD56 and CD3 positive cells and R2 for HLA-DR+ myelomonocytic cells. Histograms show isotype control and LAIR-1 mAb staining to the leukocyte population discriminated (R3). Staining is representative of decidual leukocytes from 3 independent individuals. Histological staining of decidua adjacent to a first trimester implantation site is shown (B). No staining with an HLA-G mAb indicates the absence of extravillous trophoblast in this area. Immunoreactivity corresponding to LAIR-1 can be seen on maternal cells adjacent to arteries and glands or scattered throughout the decidua (right), consistent with LAIR-1 expression by maternal leukocytes. SA, spiral artery; G, uterine gland.
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fig6: LAIR-1 expression in the decidua. Decidual leukocytes isolated from first trimester pregnancies were analysed by surface flow cytometry (A). The scatter gate R1 was used for CD45, CD56 and CD3 positive cells and R2 for HLA-DR+ myelomonocytic cells. Histograms show isotype control and LAIR-1 mAb staining to the leukocyte population discriminated (R3). Staining is representative of decidual leukocytes from 3 independent individuals. Histological staining of decidua adjacent to a first trimester implantation site is shown (B). No staining with an HLA-G mAb indicates the absence of extravillous trophoblast in this area. Immunoreactivity corresponding to LAIR-1 can be seen on maternal cells adjacent to arteries and glands or scattered throughout the decidua (right), consistent with LAIR-1 expression by maternal leukocytes. SA, spiral artery; G, uterine gland.

Mentions: LAIR-2 has been shown to be a soluble antagonist of the closely related LAIR-1 receptor that is expressed by all peripheral leukocytes [26,27]. Using flow cytometry of first trimester decidual leukocytes, we found that LAIR-1 is expressed by all CD3+ and HLA-DR+ cells, and ∼50% of the CD56 + cell population (Fig. 6A). Immuno-histological staining of the decidua basalis showed that these LAIR-1+ leukocytes are present underlying glandular epithelium and around blood vessels as well as scattered throughout the decidua (Fig. 6B).


Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.

Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A - Placenta (2010)

LAIR-1 expression in the decidua. Decidual leukocytes isolated from first trimester pregnancies were analysed by surface flow cytometry (A). The scatter gate R1 was used for CD45, CD56 and CD3 positive cells and R2 for HLA-DR+ myelomonocytic cells. Histograms show isotype control and LAIR-1 mAb staining to the leukocyte population discriminated (R3). Staining is representative of decidual leukocytes from 3 independent individuals. Histological staining of decidua adjacent to a first trimester implantation site is shown (B). No staining with an HLA-G mAb indicates the absence of extravillous trophoblast in this area. Immunoreactivity corresponding to LAIR-1 can be seen on maternal cells adjacent to arteries and glands or scattered throughout the decidua (right), consistent with LAIR-1 expression by maternal leukocytes. SA, spiral artery; G, uterine gland.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065343&req=5

fig6: LAIR-1 expression in the decidua. Decidual leukocytes isolated from first trimester pregnancies were analysed by surface flow cytometry (A). The scatter gate R1 was used for CD45, CD56 and CD3 positive cells and R2 for HLA-DR+ myelomonocytic cells. Histograms show isotype control and LAIR-1 mAb staining to the leukocyte population discriminated (R3). Staining is representative of decidual leukocytes from 3 independent individuals. Histological staining of decidua adjacent to a first trimester implantation site is shown (B). No staining with an HLA-G mAb indicates the absence of extravillous trophoblast in this area. Immunoreactivity corresponding to LAIR-1 can be seen on maternal cells adjacent to arteries and glands or scattered throughout the decidua (right), consistent with LAIR-1 expression by maternal leukocytes. SA, spiral artery; G, uterine gland.
Mentions: LAIR-2 has been shown to be a soluble antagonist of the closely related LAIR-1 receptor that is expressed by all peripheral leukocytes [26,27]. Using flow cytometry of first trimester decidual leukocytes, we found that LAIR-1 is expressed by all CD3+ and HLA-DR+ cells, and ∼50% of the CD56 + cell population (Fig. 6A). Immuno-histological staining of the decidua basalis showed that these LAIR-1+ leukocytes are present underlying glandular epithelium and around blood vessels as well as scattered throughout the decidua (Fig. 6B).

Bottom Line: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray.We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction.Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

Show MeSH
Related in: MedlinePlus