Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.
Bottom Line: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray.We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction.Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.
Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.Show MeSH
Mentions: Of the 3471 probes differing significantly between EVT and VT (q < 0.01), 885 transcripts differed by more than 4-fold (listed in Suppl Table 3). A selection of these transcripts, classified into functional catagories based on gene ontology annotations is presented in Fig. 3. The genes most strikingly up-regulated in VT were solute carrier family molecules, with SLC40A1 (ferroportin-1) and SLC22A11 > 130-fold and another 5 members of this family were expressed at levels more than 10-fold higher than in EVT. Also strongly up-regulated in VT were imprinted genes, PEG3 and PEG10 (>50-fold), the adhesion molecules integrin β5, siglec 6 and FRAS1 (>30-fold), human endogenous retroviruses HERV-FRD and ERVWE1, the transcription factor ELF5 and receptors for soluble factors such as colony stimulating factor 3, ephrin receptor, EPHB4 and growth hormone receptor (all around 20-fold). Also strongly up-regulated in VT were poorly-characterised molecules such as palmitylated membrane protein 1 (MPP1) and contactin-associated protein-like 2 (CNTNAP2), both 40-fold higher in VT; lipoprotein receptor-related 5 (LRP5), the G-protein coupled receptors, GPR56 and GPR172B (all around 30-fold) and the Ig-domain receptor, ILDR1 (10-fold). Many of these represent transcripts not previously known to be highly expressed in VT.
Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.