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Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.

Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A - Placenta (2010)

Bottom Line: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray.We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction.Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

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Comparison of gene expression profiles in primary EVT and VT with the choriocarcinoma cell lines JEG-3 and JAR. Unsupervised hierarchical clustering of the four cell types was performed using transcripts identified as showing variable expression across all samples (A). Organisation and length of the dendrogram branches reflect the degree of similarity between samples. (B). Identification of specific transcripts which differ significantly in expression (q < 0.01) by at least 4-fold up or down between cell types. 885 transcripts differ between EVT and VT by these criteria, compared with only 183 between JEG and JAR. 1188 transcripts differ between EVT and JEG and 870 between VT and JAR respectively. (C). The differences in expression between EVT and VT cells are not the same as the differences between JEG and JAR. For example, of the 457 transcripts at least 4-fold lower in EVT compared with VT, only 3 of these transcripts are also lower in JEG vs JAR.
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fig1: Comparison of gene expression profiles in primary EVT and VT with the choriocarcinoma cell lines JEG-3 and JAR. Unsupervised hierarchical clustering of the four cell types was performed using transcripts identified as showing variable expression across all samples (A). Organisation and length of the dendrogram branches reflect the degree of similarity between samples. (B). Identification of specific transcripts which differ significantly in expression (q < 0.01) by at least 4-fold up or down between cell types. 885 transcripts differ between EVT and VT by these criteria, compared with only 183 between JEG and JAR. 1188 transcripts differ between EVT and JEG and 870 between VT and JAR respectively. (C). The differences in expression between EVT and VT cells are not the same as the differences between JEG and JAR. For example, of the 457 transcripts at least 4-fold lower in EVT compared with VT, only 3 of these transcripts are also lower in JEG vs JAR.

Mentions: JEG-3 expresses HLA-G, while JAR is HLA class I negative. Based on this phenotype, these choriocarcinoma cell lines are widely used as models of primary EVT and VT respectively. The expression profiles of primary EVT and VT cells were compared with the corresponding cell lines JEG-3 and JAR. Unsupervised hierarchical clustering produced a dendrogram in which each cell type formed individual groups, indicating a distinctive expression profile, but the two choriocarcinoma lines clustered together on a separate branch from either primary cell type (Fig. 1A). Over 850 transcripts differed significantly (q < 0.01) by at least 4-fold between EVT and VT, EVT and JEG-3, VT and JAR; whereas only 183 transcripts differed between JEG and JAR (Fig. 1B). Although HLA-G was up-regulated in both EVT and JEG-3 compared VT and JAR, the genes differentially expressed between the two lineages of primary trophoblast were not represented by those transcripts differing between the JEG-3 and JAR cell lines (Fig. 1C). Indeed, more transcripts differ between EVT and the ‘corresponding’ cell line JEG-3 than between EVT and VT. These choriocarcinoma cell lines appear, at the transcriptional level, to be poorly representative of the corresponding VT and EVT.


Genome-wide expression profile of first trimester villous and extravillous human trophoblast cells.

Apps R, Sharkey A, Gardner L, Male V, Trotter M, Miller N, North R, Founds S, Moffett A - Placenta (2010)

Comparison of gene expression profiles in primary EVT and VT with the choriocarcinoma cell lines JEG-3 and JAR. Unsupervised hierarchical clustering of the four cell types was performed using transcripts identified as showing variable expression across all samples (A). Organisation and length of the dendrogram branches reflect the degree of similarity between samples. (B). Identification of specific transcripts which differ significantly in expression (q < 0.01) by at least 4-fold up or down between cell types. 885 transcripts differ between EVT and VT by these criteria, compared with only 183 between JEG and JAR. 1188 transcripts differ between EVT and JEG and 870 between VT and JAR respectively. (C). The differences in expression between EVT and VT cells are not the same as the differences between JEG and JAR. For example, of the 457 transcripts at least 4-fold lower in EVT compared with VT, only 3 of these transcripts are also lower in JEG vs JAR.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065343&req=5

fig1: Comparison of gene expression profiles in primary EVT and VT with the choriocarcinoma cell lines JEG-3 and JAR. Unsupervised hierarchical clustering of the four cell types was performed using transcripts identified as showing variable expression across all samples (A). Organisation and length of the dendrogram branches reflect the degree of similarity between samples. (B). Identification of specific transcripts which differ significantly in expression (q < 0.01) by at least 4-fold up or down between cell types. 885 transcripts differ between EVT and VT by these criteria, compared with only 183 between JEG and JAR. 1188 transcripts differ between EVT and JEG and 870 between VT and JAR respectively. (C). The differences in expression between EVT and VT cells are not the same as the differences between JEG and JAR. For example, of the 457 transcripts at least 4-fold lower in EVT compared with VT, only 3 of these transcripts are also lower in JEG vs JAR.
Mentions: JEG-3 expresses HLA-G, while JAR is HLA class I negative. Based on this phenotype, these choriocarcinoma cell lines are widely used as models of primary EVT and VT respectively. The expression profiles of primary EVT and VT cells were compared with the corresponding cell lines JEG-3 and JAR. Unsupervised hierarchical clustering produced a dendrogram in which each cell type formed individual groups, indicating a distinctive expression profile, but the two choriocarcinoma lines clustered together on a separate branch from either primary cell type (Fig. 1A). Over 850 transcripts differed significantly (q < 0.01) by at least 4-fold between EVT and VT, EVT and JEG-3, VT and JAR; whereas only 183 transcripts differed between JEG and JAR (Fig. 1B). Although HLA-G was up-regulated in both EVT and JEG-3 compared VT and JAR, the genes differentially expressed between the two lineages of primary trophoblast were not represented by those transcripts differing between the JEG-3 and JAR cell lines (Fig. 1C). Indeed, more transcripts differ between EVT and the ‘corresponding’ cell line JEG-3 than between EVT and VT. These choriocarcinoma cell lines appear, at the transcriptional level, to be poorly representative of the corresponding VT and EVT.

Bottom Line: We have examined the transcriptional changes associated with differentiation from villous to extravillous trophoblast using a whole genome microarray.We show that decidual T-cells, macrophages and NK cells express the inhibitory collagen receptor LAIR-1 and that EVT secrete LAIR-2, which can block this interaction.Since LAIR-2 is detectable in the urine of pregnant, but not non-pregnant women, trophoblast-derived LAIR-2 may also have systemic effects during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology and Centre for Trophoblast Research, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

Show MeSH
Related in: MedlinePlus