Limits...
Evaluation of ability of dentifrices to remineralize artificial caries-like lesions.

Damle SG, Bengude V, Saini S - Dent Res J (Isfahan) (2010)

Bottom Line: The results were statistically analyzed using the student t test.P value less than 0.05 was considered significant.The average hardness recovery for the experimental group was significantly higher than that in the control group (P < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Professor, Department of Pediatric Dentistry, M. M. College, Maharishi Markandeshwar University, Mullana, Ambala, India.

ABSTRACT

Background: Human endeavors to prevent dental caries have led to the use of different modalities and agents, the most commonly used of which is fluoridated dentifrice. An in situ study was carried out to evaluate the efficacy of fluoridated dentifrices in achieving remineralization of initial caries-like lesions using surface microhardness measurements and to study the qualitative changes by scan-ning electron microscopy.

Methods: Sixteen children 12-16 years of age wore a specially fabricated appliance with an artificially demineralized enamel slab for 24 hours a day, for four weeks. The children were divided into two groups, A and B. Following one week use of placebo dentifrice by both groups, group A used a fluoride dentifrice containing l000 ppm sodium monofluorophosphate, whereas group B used a placebo twice daily for 5 minutes for 21 days. Surface microhardness test carried out using a Knoop diamond indenter followed by scanning electron microscopy to evaluate the lesions. The results were statistically analyzed using the student t test. P value less than 0.05 was considered significant.

Results: The average hardness recovery for the experimental group was significantly higher than that in the control group (P < 0.001). Scanning electron microscopy revealed that fluoride significantly enhances remineralization of initial caries-like lesions.

Conclusion: Regular use of fluoridated dentifrices significantly enhances remineralization of white spot lesions.

No MeSH data available.


Related in: MedlinePlus

It shows a patient wearing the intraoral appliance with the enamel slab
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3065342&req=5

Figure 1: It shows a patient wearing the intraoral appliance with the enamel slab

Mentions: Enamel samples were prepared by cutting 4-mm-diameter cores from 34 human premolars extracted for orthodontic purpose using a diamond core drill. The teeth, collected from local dentists, were stored until use in a 1.8% formaldehyde solution, maintained above pH 7.5. The enamel cores were mounted in ¼ inchdiameter -tal acrylic covering all sides except the surface. At least 40 µm of the enamel surface were removed by polishing with 600 grit silicon carbide water slurry. The surface was then polished to a mirror finish using gamma alumina. Any enamel cores found to have surface imperfections or white spots were rejected. The enamel cores were tested for microhardness values before decalcification. The enamel was decalcified by suspending each lucite rod in a demineralizing solution for 48 hours at 37ଌ, after which the solution was changed and the slabs were kept for another 3 days.The solution consisted of 3.1 mmol/L calcium chloride, 3.1 mmol/L sodium dihydrogen orthophosphate and 50 mmol/L glacial acetic acid. pH of the solution was adjusted to 4.5 using 1 mol/L sodium hydroxide. This method produced artificial lesions similar to natural white spots.10 Lesion depths were approximately 120 µm. The enamel cores were removed from the lucite rod and cut to a 3-mm thickness to give a 4 × 4 × 3 mm slab. The enamel slabs thus produced and were incorporated in the left and right buccal flanges of specially fabricated appliances. The appliance design was a modification of the original intraoral cariogenicity test appliance designed by Koulourides.12 The appliances were designed to hold two enamel slabs in different proximal positions adjacent to natural teeth (Figure 1). The dentifrices used for this investigation were: (1) dentifrice containing 1000 ppm sodium monofluorophosphate and a fluoride-compatible silica abrasive system (2) a nonfluoride-silica abrasive placebo. Dentifrices were formulated to be similar in color and flavor and were packed in 5-oz. white tubes.


Evaluation of ability of dentifrices to remineralize artificial caries-like lesions.

Damle SG, Bengude V, Saini S - Dent Res J (Isfahan) (2010)

It shows a patient wearing the intraoral appliance with the enamel slab
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065342&req=5

Figure 1: It shows a patient wearing the intraoral appliance with the enamel slab
Mentions: Enamel samples were prepared by cutting 4-mm-diameter cores from 34 human premolars extracted for orthodontic purpose using a diamond core drill. The teeth, collected from local dentists, were stored until use in a 1.8% formaldehyde solution, maintained above pH 7.5. The enamel cores were mounted in ¼ inchdiameter -tal acrylic covering all sides except the surface. At least 40 µm of the enamel surface were removed by polishing with 600 grit silicon carbide water slurry. The surface was then polished to a mirror finish using gamma alumina. Any enamel cores found to have surface imperfections or white spots were rejected. The enamel cores were tested for microhardness values before decalcification. The enamel was decalcified by suspending each lucite rod in a demineralizing solution for 48 hours at 37ଌ, after which the solution was changed and the slabs were kept for another 3 days.The solution consisted of 3.1 mmol/L calcium chloride, 3.1 mmol/L sodium dihydrogen orthophosphate and 50 mmol/L glacial acetic acid. pH of the solution was adjusted to 4.5 using 1 mol/L sodium hydroxide. This method produced artificial lesions similar to natural white spots.10 Lesion depths were approximately 120 µm. The enamel cores were removed from the lucite rod and cut to a 3-mm thickness to give a 4 × 4 × 3 mm slab. The enamel slabs thus produced and were incorporated in the left and right buccal flanges of specially fabricated appliances. The appliance design was a modification of the original intraoral cariogenicity test appliance designed by Koulourides.12 The appliances were designed to hold two enamel slabs in different proximal positions adjacent to natural teeth (Figure 1). The dentifrices used for this investigation were: (1) dentifrice containing 1000 ppm sodium monofluorophosphate and a fluoride-compatible silica abrasive system (2) a nonfluoride-silica abrasive placebo. Dentifrices were formulated to be similar in color and flavor and were packed in 5-oz. white tubes.

Bottom Line: The results were statistically analyzed using the student t test.P value less than 0.05 was considered significant.The average hardness recovery for the experimental group was significantly higher than that in the control group (P < 0.001).

View Article: PubMed Central - PubMed

Affiliation: Professor, Department of Pediatric Dentistry, M. M. College, Maharishi Markandeshwar University, Mullana, Ambala, India.

ABSTRACT

Background: Human endeavors to prevent dental caries have led to the use of different modalities and agents, the most commonly used of which is fluoridated dentifrice. An in situ study was carried out to evaluate the efficacy of fluoridated dentifrices in achieving remineralization of initial caries-like lesions using surface microhardness measurements and to study the qualitative changes by scan-ning electron microscopy.

Methods: Sixteen children 12-16 years of age wore a specially fabricated appliance with an artificially demineralized enamel slab for 24 hours a day, for four weeks. The children were divided into two groups, A and B. Following one week use of placebo dentifrice by both groups, group A used a fluoride dentifrice containing l000 ppm sodium monofluorophosphate, whereas group B used a placebo twice daily for 5 minutes for 21 days. Surface microhardness test carried out using a Knoop diamond indenter followed by scanning electron microscopy to evaluate the lesions. The results were statistically analyzed using the student t test. P value less than 0.05 was considered significant.

Results: The average hardness recovery for the experimental group was significantly higher than that in the control group (P < 0.001). Scanning electron microscopy revealed that fluoride significantly enhances remineralization of initial caries-like lesions.

Conclusion: Regular use of fluoridated dentifrices significantly enhances remineralization of white spot lesions.

No MeSH data available.


Related in: MedlinePlus