Limits...
PerioGlas® Acts on Human Stem Cells Isolated from Peripheral Blood.

Sollazzo V, Palmieri A, Scapoli L, Martinelli M, Girardi A, Pezzetti F, Morselli P, Farinella F, Carinci F - Dent Res J (Isfahan) (2010)

Bottom Line: Pearson's chi-square (χ(2)) test was used to detect markers with significant differences in gene expression.PG has a differentiation effect on mesenchymal stem cells derived from peripheral blood.The obtained results can be relevant to better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

View Article: PubMed Central - PubMed

Affiliation: Assistant Professor, Orthopedic Clinic, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: PerioGlas® (PG) is an alloplastic material used for grafting periodontal osseous defects since 1995. In animal models, it has been proven that PG achieves histologically good repair of sur-gically created defects. In clinical trials, PG was effective as an adjunct to conventional surgery in the treatment of intrabony defects. Because the molecular events due to PG that are able to alter osteob-last activity to promote bone formation are poorly understood, we investigated the expression of os-teoblastic related genes in mesenchymal stem cells exposed to PG.

Methods: The expression levels of bone related genes like RUNX2, SP7, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1 and mesenchymal stem cells marker (CD105) were analyzed, using real time reverse transcription-polymerase chain reaction. Pearson's chi-square (χ(2)) test was used to detect markers with significant differences in gene expression.

Results: PG caused induction of osteoblast transcriptional factor (like RUNX2), bone related genes osteopontin (SPP1), osteocalcin (BGLAP) and alkaline phosphatase (ALPL). All had statistical sig-nificant P values (< 0.05).

Conclusion: PG has a differentiation effect on mesenchymal stem cells derived from peripheral blood. The obtained results can be relevant to better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

No MeSH data available.


Gene expression analysis of PB-hMSCs after 7 days of treatment with PG
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3065339&req=5

Figure 2: Gene expression analysis of PB-hMSCs after 7 days of treatment with PG

Mentions: PB-hMSCs were characterized by immunofluorescence. The cell surfaces were positive for mesen-chymal stem cell markers, CD105, CD90 and CD73 and negative for marker of hematopoietic origin, CD34 (Figure 1). Transcriptional expressions of several osteoblast-related genes (RUNX2, SP7, SPP1, COLIA1, COL3A1, BGLAP, ALPL and FOSL1) and mesenchymal stem cells marker (CD105) were examined after 7 days of supplement treatment with PG (0.04 g/ml) (Figure 2). PG enhanced the expression of the transcriptional factor RUNX2 (P < 0.01), and of several bone related genes like FOSL1 (P was not significant), ALPL (P < 0.049), BGLAP (P < 0.027) and SPP1 (P < 0.039). The mesenchymal related marker CD105 (P < 0.017) was up regulated in treated cells respected as control. At the contrary, the two collagens COLIA1 (P < 0.01), COL3A1 (P < 0.042) and zinc finger transcription factor SP7 (p was not significant) were downregulated.


PerioGlas® Acts on Human Stem Cells Isolated from Peripheral Blood.

Sollazzo V, Palmieri A, Scapoli L, Martinelli M, Girardi A, Pezzetti F, Morselli P, Farinella F, Carinci F - Dent Res J (Isfahan) (2010)

Gene expression analysis of PB-hMSCs after 7 days of treatment with PG
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065339&req=5

Figure 2: Gene expression analysis of PB-hMSCs after 7 days of treatment with PG
Mentions: PB-hMSCs were characterized by immunofluorescence. The cell surfaces were positive for mesen-chymal stem cell markers, CD105, CD90 and CD73 and negative for marker of hematopoietic origin, CD34 (Figure 1). Transcriptional expressions of several osteoblast-related genes (RUNX2, SP7, SPP1, COLIA1, COL3A1, BGLAP, ALPL and FOSL1) and mesenchymal stem cells marker (CD105) were examined after 7 days of supplement treatment with PG (0.04 g/ml) (Figure 2). PG enhanced the expression of the transcriptional factor RUNX2 (P < 0.01), and of several bone related genes like FOSL1 (P was not significant), ALPL (P < 0.049), BGLAP (P < 0.027) and SPP1 (P < 0.039). The mesenchymal related marker CD105 (P < 0.017) was up regulated in treated cells respected as control. At the contrary, the two collagens COLIA1 (P < 0.01), COL3A1 (P < 0.042) and zinc finger transcription factor SP7 (p was not significant) were downregulated.

Bottom Line: Pearson's chi-square (χ(2)) test was used to detect markers with significant differences in gene expression.PG has a differentiation effect on mesenchymal stem cells derived from peripheral blood.The obtained results can be relevant to better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

View Article: PubMed Central - PubMed

Affiliation: Assistant Professor, Orthopedic Clinic, University of Ferrara, Ferrara, Italy.

ABSTRACT

Background: PerioGlas® (PG) is an alloplastic material used for grafting periodontal osseous defects since 1995. In animal models, it has been proven that PG achieves histologically good repair of sur-gically created defects. In clinical trials, PG was effective as an adjunct to conventional surgery in the treatment of intrabony defects. Because the molecular events due to PG that are able to alter osteob-last activity to promote bone formation are poorly understood, we investigated the expression of os-teoblastic related genes in mesenchymal stem cells exposed to PG.

Methods: The expression levels of bone related genes like RUNX2, SP7, SPP1, COL1A1, COL3A1, BGLAP, ALPL, and FOSL1 and mesenchymal stem cells marker (CD105) were analyzed, using real time reverse transcription-polymerase chain reaction. Pearson's chi-square (χ(2)) test was used to detect markers with significant differences in gene expression.

Results: PG caused induction of osteoblast transcriptional factor (like RUNX2), bone related genes osteopontin (SPP1), osteocalcin (BGLAP) and alkaline phosphatase (ALPL). All had statistical sig-nificant P values (< 0.05).

Conclusion: PG has a differentiation effect on mesenchymal stem cells derived from peripheral blood. The obtained results can be relevant to better understanding of the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

No MeSH data available.