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A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san.

Weon JB, Yang HJ, Ma JY, Ma CJ - Pharmacogn Mag (2011)

Bottom Line: The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%.In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, South Korea.

ABSTRACT

Background: Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.

Objective: For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.

Materials and methods: To develop the method, a reverse phase column, DIONEX C (18) (5 μm, 120 µ, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.

Results: All calibration curves of components showed good linearity (R (2) > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 μg/ml and 0.04 to 0.53 μg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.

Conclusion: This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

No MeSH data available.


Related in: MedlinePlus

The HPLC chromatogram of the Bangpungtongsung-san sample (1: BPTSS – A, 2: BPTSS – B, 3: BPTSS – C, 4: BPTSS – D, 5: BPTSS – E)
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Figure 0003: The HPLC chromatogram of the Bangpungtongsung-san sample (1: BPTSS – A, 2: BPTSS – B, 3: BPTSS – C, 4: BPTSS – D, 5: BPTSS – E)

Mentions: The developed HPLC method was applied to the prepared Bangpungtongsung-san sample and four samples were obtained from different herbal medicine companies [Figure 3]. The contents of five marker components were calculated from the calibration curve of the standards. The results indicated that differences appeared in the amounts of marker components in Bangpungtongsung-san samples A-E. The content of marker components in herbs might have been affected by many factors such as collection time or cultivation environment and the manufacturing process of the prescription such as Bangpungtongsung-san [Table 4].


A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san.

Weon JB, Yang HJ, Ma JY, Ma CJ - Pharmacogn Mag (2011)

The HPLC chromatogram of the Bangpungtongsung-san sample (1: BPTSS – A, 2: BPTSS – B, 3: BPTSS – C, 4: BPTSS – D, 5: BPTSS – E)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065159&req=5

Figure 0003: The HPLC chromatogram of the Bangpungtongsung-san sample (1: BPTSS – A, 2: BPTSS – B, 3: BPTSS – C, 4: BPTSS – D, 5: BPTSS – E)
Mentions: The developed HPLC method was applied to the prepared Bangpungtongsung-san sample and four samples were obtained from different herbal medicine companies [Figure 3]. The contents of five marker components were calculated from the calibration curve of the standards. The results indicated that differences appeared in the amounts of marker components in Bangpungtongsung-san samples A-E. The content of marker components in herbs might have been affected by many factors such as collection time or cultivation environment and the manufacturing process of the prescription such as Bangpungtongsung-san [Table 4].

Bottom Line: The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%.In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, South Korea.

ABSTRACT

Background: Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.

Objective: For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.

Materials and methods: To develop the method, a reverse phase column, DIONEX C (18) (5 μm, 120 µ, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.

Results: All calibration curves of components showed good linearity (R (2) > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 μg/ml and 0.04 to 0.53 μg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.

Conclusion: This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

No MeSH data available.


Related in: MedlinePlus