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A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san.

Weon JB, Yang HJ, Ma JY, Ma CJ - Pharmacogn Mag (2011)

Bottom Line: The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%.In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, South Korea.

ABSTRACT

Background: Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.

Objective: For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.

Materials and methods: To develop the method, a reverse phase column, DIONEX C (18) (5 μm, 120 µ, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.

Results: All calibration curves of components showed good linearity (R (2) > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 μg/ml and 0.04 to 0.53 μg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.

Conclusion: This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

No MeSH data available.


Related in: MedlinePlus

The HPLC chromatogram of the standard mixture (a) and Bangpuntongsung-san sample (b)
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Figure 0002: The HPLC chromatogram of the standard mixture (a) and Bangpuntongsung-san sample (b)

Mentions: To precisely and exactly analyze five marker components in Banhpungtongsung-san, a suitable HPLC system condition, such as chromatographic column, mobile phase elution system, UV wavelength of detector, was established. In general, a reverse phase column was applied to assay chemical components in natural products. We selected C 18 as one. The mobile phase consisting of methanol (A) and water (B) was tested in various gradient systems and an adequate gradient ratio was selected. The gradient volume of solvent B was 75-70% at 0-10 min, 70-50% at 10-20 min, 50-40% at 20-30 min and 40-30% at 30-50 min. The UV wavelength of the DAD detector was tested at 230, 240, 254, and 280 nm. A wavelength of each component selected most of the absorbed UV wavelength in the UV spectrum. Paeoniflorin, 6-gingerol, and decursin were 230 nm, geniposide was 240 nm, and glycyrrhizin was 254 nm. The peak of each compound was confirmed by comparing the retention time and UV spectrum of each marker constituent. Retention times for peaks of geniposide, paeoniflorin, glycyrrhizin, 6-gingerol, and decursin were 10.03, 12.10, 20.83, 35.81, and 44.51 min, respectively [Figure 2a]. The Bangpungtongsung-san sample was analyzed by this HPLC method and a chromatogram of sample showed that five marker components were separated effectively [Figure 2b].


A HPLC-DAD method for the simultaneous determination of five marker components in the traditional herbal medicine Bangpungtongsung-san.

Weon JB, Yang HJ, Ma JY, Ma CJ - Pharmacogn Mag (2011)

The HPLC chromatogram of the standard mixture (a) and Bangpuntongsung-san sample (b)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065159&req=5

Figure 0002: The HPLC chromatogram of the standard mixture (a) and Bangpuntongsung-san sample (b)
Mentions: To precisely and exactly analyze five marker components in Banhpungtongsung-san, a suitable HPLC system condition, such as chromatographic column, mobile phase elution system, UV wavelength of detector, was established. In general, a reverse phase column was applied to assay chemical components in natural products. We selected C 18 as one. The mobile phase consisting of methanol (A) and water (B) was tested in various gradient systems and an adequate gradient ratio was selected. The gradient volume of solvent B was 75-70% at 0-10 min, 70-50% at 10-20 min, 50-40% at 20-30 min and 40-30% at 30-50 min. The UV wavelength of the DAD detector was tested at 230, 240, 254, and 280 nm. A wavelength of each component selected most of the absorbed UV wavelength in the UV spectrum. Paeoniflorin, 6-gingerol, and decursin were 230 nm, geniposide was 240 nm, and glycyrrhizin was 254 nm. The peak of each compound was confirmed by comparing the retention time and UV spectrum of each marker constituent. Retention times for peaks of geniposide, paeoniflorin, glycyrrhizin, 6-gingerol, and decursin were 10.03, 12.10, 20.83, 35.81, and 44.51 min, respectively [Figure 2a]. The Bangpungtongsung-san sample was analyzed by this HPLC method and a chromatogram of sample showed that five marker components were separated effectively [Figure 2b].

Bottom Line: The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%.In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomaterials Engineering, Division of Biotechnology and Bioengineering, Kangwon National University, Chuncheon 200-701, South Korea.

ABSTRACT

Background: Bangpungtongsung-san, one of the traditional herbal medicines, was known to be a prescription for obesity.

Objective: For the simultaneous determination of five components (paeoniflorin, 6-gingerol, decursin, geniposide, and glycyrrhizin) in Bangpungtongsung-san, a high-performance liquid chromatography with diode-array detector method was established.

Materials and methods: To develop the method, a reverse phase column, DIONEX C (18) (5 μm, 120 µ, 4.6 mm × 150 mm) was used. The mobile phase consisted of methanol and water using a gradient elution. The UV wavelength was set at 230, 240, and 254 nm. Method validation was accomplished by linearity, precision test, and recovery test.

Results: All calibration curves of components showed good linearity (R (2) > 0.9959). The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01 to 0.17 μg/ml and 0.04 to 0.53 μg/ml, respectively. The relative standard deviations (RSD) value of precision test, intraday and interday tests were less than 0.43% and 1.26%. In the recovery test, results of accuracy ranged from 95.27% to 107.70% with RSD values less than 2.21%.

Conclusion: This developed method was applied to the commercial Bangpungtongsung-san sample and the five marker components were separated effectively without interference of any peaks of components.

No MeSH data available.


Related in: MedlinePlus