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Honey induces apoptosis in renal cell carcinoma.

Samarghandian S, Afshari JT, Davoodi S - Pharmacogn Mag (2011)

Bottom Line: Honey decreased the cell viability in the malignant cells in a concentration- and time-dependent manner.It might be concluded that honey may cause cell death in the ACHN cells, in which apoptosis plays an important role.Most of the drugs used in the cancer treatment are apoptotic inducers, hence apoptotic nature of honey is considered vital.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Mashhad University Medical Sciences, Mashhad,, Iran.

ABSTRACT

Background: The fact that antioxidants have several preventative effects against different diseases, such as coronary diseases, inflammatory disorders, neurologic degeneration, aging, and cancer, has led to the search for food rich in antioxidants. Honey has been used as a traditional food and medical source since ancient times. However, recently many scientists have been concentrating on the antioxidant property of honey. By use of human renal cancer cell lines (ACHN), we investigated the antiproliferative activity, apoptosis, and the antitumor activity of honey.

Materials and methods: The cells were cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum treated with different concentrations of honey for 3 consecutive days. Cell viability was quantitated by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptotic cells were determined using Annexin-V-fluorescein isothiocyanate (FITC) by flow cytometry.

Results: Honey decreased the cell viability in the malignant cells in a concentration- and time-dependent manner. The IC (50) values against the ACHN cell lines were determined as 1.7 ± 0.04% and 2.1 ± 0.03% μg/mL after 48 and 72 h, respectively. Honey induced apoptosis of the ACHN cells in a concentration-dependent manner, as determined by flow cytometry histogram of treated cells.

Conclusion: It might be concluded that honey may cause cell death in the ACHN cells, in which apoptosis plays an important role. Most of the drugs used in the cancer treatment are apoptotic inducers, hence apoptotic nature of honey is considered vital. Therefore, it prompted us to investigate honey as a potential candidate for renal cancer treatment.

No MeSH data available.


Related in: MedlinePlus

Effect of honey extract on cell viability of ACHN cells. Cells were treated with different concentrations of honey extract for 24, 48, and 72 h. Viability was quantitated by MTT assay. Results are mean ± SEM. The asterisks (***P < 0.001) are indicator of statistical difference obtained separately at different time points compared with their controls
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Figure 0001: Effect of honey extract on cell viability of ACHN cells. Cells were treated with different concentrations of honey extract for 24, 48, and 72 h. Viability was quantitated by MTT assay. Results are mean ± SEM. The asterisks (***P < 0.001) are indicator of statistical difference obtained separately at different time points compared with their controls

Mentions: ACHN cell line was incubated with various concentrations of honey for 24, 48, and 72 h. The impact of honey on the cell viability was quantitated by MTT assay. Exposure of the ACHN cells for 24, 48, and 72 h with honey showed significantly high growth inhibitory effects on renal carcinoma cell line in a concentration- and time-dependent manner (P < 0.001). Although there was no significant result at a low concentration of honey (2.5%) after 24 h, the exposure of ACHN cell line for 24 h decreased the number of viable cells at higher doses of honey (5%, 10%, 20%) (P < 0.001) vs control. On the other hand, treatment of ACHN cell lines for 48 and 72 h with different doses of honey (2.5%, 5%, 10%, 20%) resulted in a marked reduction in the number of viable cells (P < 0.001) [Figure 1]. The dose inducing 50% cell growth inhibition (IC50) against malignant cells was determined at 1.7% ± 0.04% and 2.1% ± 0.03% after 48 and 72 h, respectively [Table 1].


Honey induces apoptosis in renal cell carcinoma.

Samarghandian S, Afshari JT, Davoodi S - Pharmacogn Mag (2011)

Effect of honey extract on cell viability of ACHN cells. Cells were treated with different concentrations of honey extract for 24, 48, and 72 h. Viability was quantitated by MTT assay. Results are mean ± SEM. The asterisks (***P < 0.001) are indicator of statistical difference obtained separately at different time points compared with their controls
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065157&req=5

Figure 0001: Effect of honey extract on cell viability of ACHN cells. Cells were treated with different concentrations of honey extract for 24, 48, and 72 h. Viability was quantitated by MTT assay. Results are mean ± SEM. The asterisks (***P < 0.001) are indicator of statistical difference obtained separately at different time points compared with their controls
Mentions: ACHN cell line was incubated with various concentrations of honey for 24, 48, and 72 h. The impact of honey on the cell viability was quantitated by MTT assay. Exposure of the ACHN cells for 24, 48, and 72 h with honey showed significantly high growth inhibitory effects on renal carcinoma cell line in a concentration- and time-dependent manner (P < 0.001). Although there was no significant result at a low concentration of honey (2.5%) after 24 h, the exposure of ACHN cell line for 24 h decreased the number of viable cells at higher doses of honey (5%, 10%, 20%) (P < 0.001) vs control. On the other hand, treatment of ACHN cell lines for 48 and 72 h with different doses of honey (2.5%, 5%, 10%, 20%) resulted in a marked reduction in the number of viable cells (P < 0.001) [Figure 1]. The dose inducing 50% cell growth inhibition (IC50) against malignant cells was determined at 1.7% ± 0.04% and 2.1% ± 0.03% after 48 and 72 h, respectively [Table 1].

Bottom Line: Honey decreased the cell viability in the malignant cells in a concentration- and time-dependent manner.It might be concluded that honey may cause cell death in the ACHN cells, in which apoptosis plays an important role.Most of the drugs used in the cancer treatment are apoptotic inducers, hence apoptotic nature of honey is considered vital.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Mashhad University Medical Sciences, Mashhad,, Iran.

ABSTRACT

Background: The fact that antioxidants have several preventative effects against different diseases, such as coronary diseases, inflammatory disorders, neurologic degeneration, aging, and cancer, has led to the search for food rich in antioxidants. Honey has been used as a traditional food and medical source since ancient times. However, recently many scientists have been concentrating on the antioxidant property of honey. By use of human renal cancer cell lines (ACHN), we investigated the antiproliferative activity, apoptosis, and the antitumor activity of honey.

Materials and methods: The cells were cultured in Dulbecco's modified Eagle's medium with 10% fetal bovine serum treated with different concentrations of honey for 3 consecutive days. Cell viability was quantitated by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Apoptotic cells were determined using Annexin-V-fluorescein isothiocyanate (FITC) by flow cytometry.

Results: Honey decreased the cell viability in the malignant cells in a concentration- and time-dependent manner. The IC (50) values against the ACHN cell lines were determined as 1.7 ± 0.04% and 2.1 ± 0.03% μg/mL after 48 and 72 h, respectively. Honey induced apoptosis of the ACHN cells in a concentration-dependent manner, as determined by flow cytometry histogram of treated cells.

Conclusion: It might be concluded that honey may cause cell death in the ACHN cells, in which apoptosis plays an important role. Most of the drugs used in the cancer treatment are apoptotic inducers, hence apoptotic nature of honey is considered vital. Therefore, it prompted us to investigate honey as a potential candidate for renal cancer treatment.

No MeSH data available.


Related in: MedlinePlus