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The relaxant effect induced by Allium sativum L. bulb aqueous extract on rat isolated trachea.

Fehri B, Ahmed MK, Aiache JM - Pharmacogn Mag (2011)

Bottom Line: Garlic is also known for its beneficial effects on the cardiovascular system.It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7).It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Tunisia Pharmaceutical Industries Company (SIPHAT), Fondouk Choucha-Radès, 2013 Ben Arous, Tunisia.

ABSTRACT

Background: Garlic plays an important role in complementary and alternative medicine. Most people believe in and use herbal products even when they have not been as thoroughly researched as garlic. Garlic is also known for its beneficial effects on the cardiovascular system.

Materials and methods: The relaxant effect of Allium sativum L. bulb aqueous extract (ASBAE) containing 0.06%-0.10% of allicin was studied on isolated smooth muscle of trachea of rats precontracted using acetylcholine (10(-5) M).

Results: It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7). Pretreatments with mepyramine (10(-7) M), methysergide (10(-7) M), caffeine (10(-6) M), theophylline (10(-6) M), nifedipine (10(-6) M), and dipyridamole (10(-6) M) did not alter ASBAE concentration-response curves. In turn, concentration-response curves to ASBAE were significantly shifted toward right in the presence of aspirin (3.10(-3) M), indomethacin (10(-6) M), prazosin (10(-6) M), and propranolol (10(-7) M).

Conclusion: It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

No MeSH data available.


Related in: MedlinePlus

Cumulative concentration-response curves for Allium sativum L. extract on rat isolated trachea. Values are mean ± SEM
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Figure 0002: Cumulative concentration-response curves for Allium sativum L. extract on rat isolated trachea. Values are mean ± SEM

Mentions: Pretreatments with mepyramine 10−7 M and methysergide 10−7 M, ASBAE concentration-response curves were not significantly modified as observed by pD2 values [Table 1]. Statistical analysis of pD2 values also failed to detect any difference in the relaxant effect of ASBAE between control and tracheal rings treated with caffeine (10−6 M), theophylline (3.10−6 M), nifedipine (10−6 M), and dipyridamole (10−6 M) [Table 1]. In turn, pretreatments with aspirin (3.10−3 M) and indomethacin (10−6 M) caused right shift to ASBAE concentration-response curves, and pD2 values significantly decreased [Table 1 and Figure 2]. Prazosin (10−6 M) and propranolol (10−7 M) had the same effect (P < 0.05 and P < 0.001) [Table 1 and Figure 3].


The relaxant effect induced by Allium sativum L. bulb aqueous extract on rat isolated trachea.

Fehri B, Ahmed MK, Aiache JM - Pharmacogn Mag (2011)

Cumulative concentration-response curves for Allium sativum L. extract on rat isolated trachea. Values are mean ± SEM
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3065150&req=5

Figure 0002: Cumulative concentration-response curves for Allium sativum L. extract on rat isolated trachea. Values are mean ± SEM
Mentions: Pretreatments with mepyramine 10−7 M and methysergide 10−7 M, ASBAE concentration-response curves were not significantly modified as observed by pD2 values [Table 1]. Statistical analysis of pD2 values also failed to detect any difference in the relaxant effect of ASBAE between control and tracheal rings treated with caffeine (10−6 M), theophylline (3.10−6 M), nifedipine (10−6 M), and dipyridamole (10−6 M) [Table 1]. In turn, pretreatments with aspirin (3.10−3 M) and indomethacin (10−6 M) caused right shift to ASBAE concentration-response curves, and pD2 values significantly decreased [Table 1 and Figure 2]. Prazosin (10−6 M) and propranolol (10−7 M) had the same effect (P < 0.05 and P < 0.001) [Table 1 and Figure 3].

Bottom Line: Garlic is also known for its beneficial effects on the cardiovascular system.It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7).It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Tunisia Pharmaceutical Industries Company (SIPHAT), Fondouk Choucha-Radès, 2013 Ben Arous, Tunisia.

ABSTRACT

Background: Garlic plays an important role in complementary and alternative medicine. Most people believe in and use herbal products even when they have not been as thoroughly researched as garlic. Garlic is also known for its beneficial effects on the cardiovascular system.

Materials and methods: The relaxant effect of Allium sativum L. bulb aqueous extract (ASBAE) containing 0.06%-0.10% of allicin was studied on isolated smooth muscle of trachea of rats precontracted using acetylcholine (10(-5) M).

Results: It was found that ASBAE induced a dose-dependent relaxation with recorded EC (50) values of 71.87 ± 5.90 µg/mL (n = 7). Pretreatments with mepyramine (10(-7) M), methysergide (10(-7) M), caffeine (10(-6) M), theophylline (10(-6) M), nifedipine (10(-6) M), and dipyridamole (10(-6) M) did not alter ASBAE concentration-response curves. In turn, concentration-response curves to ASBAE were significantly shifted toward right in the presence of aspirin (3.10(-3) M), indomethacin (10(-6) M), prazosin (10(-6) M), and propranolol (10(-7) M).

Conclusion: It is suggested that the recorded relaxation results are due to the release of prostaglandins E (1) and E (2) consecutively to α- and β-adrenoreceptor stimulation.

No MeSH data available.


Related in: MedlinePlus